Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples
Blue mold caused by Penicillium expansum is a major postharvest disease of pome fruit. Several mechanisms possibly involved in P. expansum pathogenicity and virulence. However, factors that mediate pathogenicity and virulence are largely not yet characterized. In this work we analyzed P. expansum predicted secretome to reveal potential genes that have a role in host-pathogen interaction. A prediction pipeline was designed using an approach that combines common effector features, transcriptomic data and homology to proteins reported to be involved in pathogenicity of other pytopathogenic fungi. Among 297 genes predicted in P. expansum secretome, 103 genes (35%) were found to code for hydrolytic enzymes. The majority of the secreted enzymes are carbohydrate-degrading enzymes among which five coding for pectin-degrading enzymes are highly induced during the infection and decay of apple fruit by P. expansum, indicating that they may represent an important aspect of pathogenicity and virulence. Applying the pipeline we have predicted 17 candidate genes coding for proteins that are most likely involved in pathogenicity and virulence. One of the top candidates is a subtilisin-related peptidase, S8 (PePRT), proteolytic enzyme highly expressed in planta, and potentially involved in authophagy process. Deletion of PePRT-coding gene resulted in reduced virulence of P. expansum on apples. Moreover, ΔPeprt exhibited decreased sporulation as well as affected mycelial morphology and internal mycelial cell structure.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2018-12-15
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Subjects: | Penicillium expansum, Virulence, Host-pathogen interaction, Secretome, Authophagy, |
Online Access: | http://hdl.handle.net/10261/173615 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100006031 http://dx.doi.org/10.13039/501100001809 http://dx.doi.org/10.13039/501100003977 |
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dig-iata-es-10261-1736152020-04-07T11:33:46Z Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples Levin, E. Kishore, A. Ballester Frutos, Ana Rosa Raphael, G. Feigenberg, O. Liu, Y. Norelli, J. González-Candelas, Luis Wisniewski, M. Droby, Samir Generalitat Valenciana Israel Science Foundation National Natural Science Foundation of China United States - Israel Binational Agricultural Research and Development Fund Ministerio de Economía y Competitividad (España) Penicillium expansum Virulence Host-pathogen interaction Secretome Authophagy Blue mold caused by Penicillium expansum is a major postharvest disease of pome fruit. Several mechanisms possibly involved in P. expansum pathogenicity and virulence. However, factors that mediate pathogenicity and virulence are largely not yet characterized. In this work we analyzed P. expansum predicted secretome to reveal potential genes that have a role in host-pathogen interaction. A prediction pipeline was designed using an approach that combines common effector features, transcriptomic data and homology to proteins reported to be involved in pathogenicity of other pytopathogenic fungi. Among 297 genes predicted in P. expansum secretome, 103 genes (35%) were found to code for hydrolytic enzymes. The majority of the secreted enzymes are carbohydrate-degrading enzymes among which five coding for pectin-degrading enzymes are highly induced during the infection and decay of apple fruit by P. expansum, indicating that they may represent an important aspect of pathogenicity and virulence. Applying the pipeline we have predicted 17 candidate genes coding for proteins that are most likely involved in pathogenicity and virulence. One of the top candidates is a subtilisin-related peptidase, S8 (PePRT), proteolytic enzyme highly expressed in planta, and potentially involved in authophagy process. Deletion of PePRT-coding gene resulted in reduced virulence of P. expansum on apples. Moreover, ΔPeprt exhibited decreased sporulation as well as affected mycelial morphology and internal mycelial cell structure. We thank Israel Science Foundation (ISF) and National Natural Science Foundation of China (NSFC): 1936/14, awarded to Prof. Samir Droby and Prof. Yongsheng Liu. Israel Binational Agricultural Research and Development Fund (BARD), US-4774-14C: awarded to Prof. Samir Droby and Dr. Michael Wisniewski, and Spanish Ministry of Economy and Competitiveness (Research Grant AGL2014-55802-R) and the Generalitat Valenciana, Spain (Grant PROMETEOII/2014/027) awarded to Dr. González-Candelas. Peer reviewed 2018-12-26T08:39:09Z 2018-12-26T08:39:09Z 2018-12-15 artículo http://purl.org/coar/resource_type/c_6501 Postharvest Biology and Technology 14: 209-220 (2019) 0925-5214 http://hdl.handle.net/10261/173615 10.1016/j.postharvbio.2018.10.011 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100006031 http://dx.doi.org/10.13039/501100001809 http://dx.doi.org/10.13039/501100003977 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-55802-R Postprint https://doi.org/10.1016/j.postharvbio.2018.10.011 Sí open Elsevier |
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Penicillium expansum Virulence Host-pathogen interaction Secretome Authophagy Penicillium expansum Virulence Host-pathogen interaction Secretome Authophagy |
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Penicillium expansum Virulence Host-pathogen interaction Secretome Authophagy Penicillium expansum Virulence Host-pathogen interaction Secretome Authophagy Levin, E. Kishore, A. Ballester Frutos, Ana Rosa Raphael, G. Feigenberg, O. Liu, Y. Norelli, J. González-Candelas, Luis Wisniewski, M. Droby, Samir Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples |
description |
Blue mold caused by Penicillium expansum is a major postharvest disease of pome fruit. Several mechanisms possibly involved in P. expansum pathogenicity and virulence. However, factors that mediate pathogenicity and virulence are largely not yet characterized. In this work we analyzed P. expansum predicted secretome to reveal potential genes that have a role in host-pathogen interaction. A prediction pipeline was designed using an approach that combines common effector features, transcriptomic data and homology to proteins reported to be involved in pathogenicity of other pytopathogenic fungi. Among 297 genes predicted in P. expansum secretome, 103 genes (35%) were found to code for hydrolytic enzymes. The majority of the secreted enzymes are carbohydrate-degrading enzymes among which five coding for pectin-degrading enzymes are highly induced during the infection and decay of apple fruit by P. expansum, indicating that they may represent an important aspect of pathogenicity and virulence. Applying the pipeline we have predicted 17 candidate genes coding for proteins that are most likely involved in pathogenicity and virulence. One of the top candidates is a subtilisin-related peptidase, S8 (PePRT), proteolytic enzyme highly expressed in planta, and potentially involved in authophagy process. Deletion of PePRT-coding gene resulted in reduced virulence of P. expansum on apples. Moreover, ΔPeprt exhibited decreased sporulation as well as affected mycelial morphology and internal mycelial cell structure. |
author2 |
Generalitat Valenciana |
author_facet |
Generalitat Valenciana Levin, E. Kishore, A. Ballester Frutos, Ana Rosa Raphael, G. Feigenberg, O. Liu, Y. Norelli, J. González-Candelas, Luis Wisniewski, M. Droby, Samir |
format |
artículo |
topic_facet |
Penicillium expansum Virulence Host-pathogen interaction Secretome Authophagy |
author |
Levin, E. Kishore, A. Ballester Frutos, Ana Rosa Raphael, G. Feigenberg, O. Liu, Y. Norelli, J. González-Candelas, Luis Wisniewski, M. Droby, Samir |
author_sort |
Levin, E. |
title |
Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples |
title_short |
Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples |
title_full |
Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples |
title_fullStr |
Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples |
title_full_unstemmed |
Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples |
title_sort |
identification of pathogenicity-related genes and the role of a subtilisin-related peptidase s8 (peprt) in authophagy and virulence of penicillium expansum on apples |
publisher |
Elsevier |
publishDate |
2018-12-15 |
url |
http://hdl.handle.net/10261/173615 http://dx.doi.org/10.13039/501100003359 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100006031 http://dx.doi.org/10.13039/501100001809 http://dx.doi.org/10.13039/501100003977 |
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