High-affinity Antibodies from a Full Penthiopyrad-mimicking Hapten and Heterologous Immunoassay Development for Fruit Juice Analysis
New-generation succinate dehydrogenase inhibitors with increased antifungal performance have been developed during the last years. Particularly, penthiopyrad has been recently introduced in the agrochemical market as a complementary fungicide for combined treatments with other active principles displaying a different mode of action. In the present study, rapid and high-throughput analytical methods for penthiopyrad residue determination in foodstuffs were developed. A novel functionalized hapten that completely mimics the molecule of penthiopyrad was designed for high-affinity and specific antibody generation. Moreover, heterologous haptens were synthesized, and the influence of their respective molecular modifications over antibody binding was evaluated by competitive enzyme-linked immunosorbent assay. Two immunoassays were optimized using alternative assay formats, affording limits of detection for penthiopyrad in buffer around 0.07 ng/mL in both cases. Performance, in terms of accuracy and precision, of the developed immunochemical methods was studied using apple juice and red and white grape juices. The obtained results showed that penthiopyrad could be determined in those food samples in compliance with European maximum residue limits.
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Format: | artículo biblioteca |
Language: | English |
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Springer
2017-06-26
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Subjects: | Target mimicking, Antibodies, Fungicide residues, ELISA, Immunoassay, |
Online Access: | http://hdl.handle.net/10261/157688 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 |
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dig-iata-es-10261-1576882018-06-26T04:30:47Z High-affinity Antibodies from a Full Penthiopyrad-mimicking Hapten and Heterologous Immunoassay Development for Fruit Juice Analysis Ceballos Alcantarilla, Eric Agulló, Consuelo Abad Fuentes, Antonio Escamilla, Mónica Abad Somovilla, Antonio Mercader Badia, Josep Vicent Ministerio de Economía y Competitividad (España) European Commission Target mimicking Antibodies Fungicide residues ELISA Immunoassay New-generation succinate dehydrogenase inhibitors with increased antifungal performance have been developed during the last years. Particularly, penthiopyrad has been recently introduced in the agrochemical market as a complementary fungicide for combined treatments with other active principles displaying a different mode of action. In the present study, rapid and high-throughput analytical methods for penthiopyrad residue determination in foodstuffs were developed. A novel functionalized hapten that completely mimics the molecule of penthiopyrad was designed for high-affinity and specific antibody generation. Moreover, heterologous haptens were synthesized, and the influence of their respective molecular modifications over antibody binding was evaluated by competitive enzyme-linked immunosorbent assay. Two immunoassays were optimized using alternative assay formats, affording limits of detection for penthiopyrad in buffer around 0.07 ng/mL in both cases. Performance, in terms of accuracy and precision, of the developed immunochemical methods was studied using apple juice and red and white grape juices. The obtained results showed that penthiopyrad could be determined in those food samples in compliance with European maximum residue limits. This work was supported by the Spanish Ministerio de Ciencia e Innovación (AGL2012-39965-C02 and AGL2015-64488-C2) and cofinanced by FEDER funds. E.C.-A. is recipient of a predoctoral fellowship from the “Atracció de Talent, VLC-CAMPUS” program of the University of Valencia. Peer reviewed 2017-11-24T08:18:37Z 2017-11-24T08:18:37Z 2017-06-26 artículo http://purl.org/coar/resource_type/c_6501 Food Analytical Methods 10: 4013 (2017) 1936-9751 http://hdl.handle.net/10261/157688 10.1007/s12161-017-0974-3 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64488-C2 Postprint https://doi.org/10.1007/s12161-017-0974-3 Sí open Springer |
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Target mimicking Antibodies Fungicide residues ELISA Immunoassay Target mimicking Antibodies Fungicide residues ELISA Immunoassay |
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Target mimicking Antibodies Fungicide residues ELISA Immunoassay Target mimicking Antibodies Fungicide residues ELISA Immunoassay Ceballos Alcantarilla, Eric Agulló, Consuelo Abad Fuentes, Antonio Escamilla, Mónica Abad Somovilla, Antonio Mercader Badia, Josep Vicent High-affinity Antibodies from a Full Penthiopyrad-mimicking Hapten and Heterologous Immunoassay Development for Fruit Juice Analysis |
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New-generation succinate dehydrogenase inhibitors with increased antifungal performance have been developed during the last years. Particularly, penthiopyrad has been recently introduced in the agrochemical market as a complementary fungicide for combined treatments with other active principles displaying a different mode of action. In the present study, rapid and high-throughput analytical methods for penthiopyrad residue determination in foodstuffs were developed. A novel functionalized hapten that completely mimics the molecule of penthiopyrad was designed for high-affinity and specific antibody generation. Moreover, heterologous haptens were synthesized, and the influence of their respective molecular modifications over antibody binding was evaluated by competitive enzyme-linked immunosorbent assay. Two immunoassays were optimized using alternative assay formats, affording limits of detection for penthiopyrad in buffer around 0.07 ng/mL in both cases. Performance, in terms of accuracy and precision, of the developed immunochemical methods was studied using apple juice and red and white grape juices. The obtained results showed that penthiopyrad could be determined in those food samples in compliance with European maximum residue limits. |
author2 |
Ministerio de Economía y Competitividad (España) |
author_facet |
Ministerio de Economía y Competitividad (España) Ceballos Alcantarilla, Eric Agulló, Consuelo Abad Fuentes, Antonio Escamilla, Mónica Abad Somovilla, Antonio Mercader Badia, Josep Vicent |
format |
artículo |
topic_facet |
Target mimicking Antibodies Fungicide residues ELISA Immunoassay |
author |
Ceballos Alcantarilla, Eric Agulló, Consuelo Abad Fuentes, Antonio Escamilla, Mónica Abad Somovilla, Antonio Mercader Badia, Josep Vicent |
author_sort |
Ceballos Alcantarilla, Eric |
title |
High-affinity Antibodies from a Full Penthiopyrad-mimicking Hapten and Heterologous Immunoassay Development for Fruit Juice Analysis |
title_short |
High-affinity Antibodies from a Full Penthiopyrad-mimicking Hapten and Heterologous Immunoassay Development for Fruit Juice Analysis |
title_full |
High-affinity Antibodies from a Full Penthiopyrad-mimicking Hapten and Heterologous Immunoassay Development for Fruit Juice Analysis |
title_fullStr |
High-affinity Antibodies from a Full Penthiopyrad-mimicking Hapten and Heterologous Immunoassay Development for Fruit Juice Analysis |
title_full_unstemmed |
High-affinity Antibodies from a Full Penthiopyrad-mimicking Hapten and Heterologous Immunoassay Development for Fruit Juice Analysis |
title_sort |
high-affinity antibodies from a full penthiopyrad-mimicking hapten and heterologous immunoassay development for fruit juice analysis |
publisher |
Springer |
publishDate |
2017-06-26 |
url |
http://hdl.handle.net/10261/157688 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 |
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