Ecological interactions among Saccharomyces cerevisiae strains: insight into the dominance phenomenon
This study investigates the behaviour of Saccharomyces cerevisiae strains, in order to obtain insight into the intraspecies competition taking place in mixed populations of this species. Two strains of S. cerevisiae, one dominant and one non-dominant, were labelled and mixed, and individual fermentations were set up to study the transcriptomes of the strains by means of RNA-seq. The results obtained suggest that cell-to-cell contact and aggregation, which are driven by the expression of genes that are associated with the cell surface, are indispensable conditions for the achievement of dominance. Observations on mixed aggregates, made up of cells of both strains, which were detected by means of flow cytometry, have confirmed the transcriptomic data. Furthermore, overexpression of the SSU1 gene, which encodes for a transporter that confers resistance to sulphites, provides an ecological advantage to the dominant strain. A mechanistic model is proposed that sheds light on the dominance phenomenon between different strains of the S. cerevisiae species. The collected data suggest that cell-to-cell contact, together with differential sulphite production and resistance is important in determining the dominance of one strain over another.
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Format: | artículo biblioteca |
Language: | English |
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Nature Publishing Group
2017-03
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Subjects: | Saccharomyces cerevisiae, Intraspecies competition, Dominance, Fermentation, Aggregation, Transcriptoma, Sulphites, |
Online Access: | http://hdl.handle.net/10261/147386 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003359 |
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dig-iata-es-10261-1473862021-12-28T15:47:45Z Ecological interactions among Saccharomyces cerevisiae strains: insight into the dominance phenomenon Pérez-Torrado, Roberto ; Rantsiou, K ; Perrone, B ; Navarro-Tapia, Elizabeth ; Querol, Amparo ; Cocolin, L. Pérez Torrado, Roberto Rantsiou, Kalliopi Perrone, Benedetta Navarro Tapia, Elisabet Querol, Amparo Cocolin, Luca Ministerio de Economía y Competitividad (España) European Commission Generalitat Valenciana Saccharomyces cerevisiae Intraspecies competition Dominance Fermentation Aggregation Transcriptoma Sulphites This study investigates the behaviour of Saccharomyces cerevisiae strains, in order to obtain insight into the intraspecies competition taking place in mixed populations of this species. Two strains of S. cerevisiae, one dominant and one non-dominant, were labelled and mixed, and individual fermentations were set up to study the transcriptomes of the strains by means of RNA-seq. The results obtained suggest that cell-to-cell contact and aggregation, which are driven by the expression of genes that are associated with the cell surface, are indispensable conditions for the achievement of dominance. Observations on mixed aggregates, made up of cells of both strains, which were detected by means of flow cytometry, have confirmed the transcriptomic data. Furthermore, overexpression of the SSU1 gene, which encodes for a transporter that confers resistance to sulphites, provides an ecological advantage to the dominant strain. A mechanistic model is proposed that sheds light on the dominance phenomenon between different strains of the S. cerevisiae species. The collected data suggest that cell-to-cell contact, together with differential sulphite production and resistance is important in determining the dominance of one strain over another. This work was supported by grants AGL2012-39937-C02-01 and AGL2015-67504-C3-1-R from the Spanish Government and FEDER and by grant PROMETEO (project AGL2015-67504-C3-1-R) from Generalitat Valenciana to A. Querol. Peer reviewed 2017-03-27T10:19:39Z 2017-03-27T10:19:39Z 2017-03 artículo http://purl.org/coar/resource_type/c_6501 Scientific Reports 7: Article number 43603 (2017) http://hdl.handle.net/10261/147386 10.1038/srep43603 2045-2322 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003359 28266552 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-67504-C3-1-R Publisher's version http://dx.doi.org/10.1038/srep43603 Sí open Nature Publishing Group |
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Saccharomyces cerevisiae Intraspecies competition Dominance Fermentation Aggregation Transcriptoma Sulphites Saccharomyces cerevisiae Intraspecies competition Dominance Fermentation Aggregation Transcriptoma Sulphites |
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Saccharomyces cerevisiae Intraspecies competition Dominance Fermentation Aggregation Transcriptoma Sulphites Saccharomyces cerevisiae Intraspecies competition Dominance Fermentation Aggregation Transcriptoma Sulphites Pérez Torrado, Roberto Rantsiou, Kalliopi Perrone, Benedetta Navarro Tapia, Elisabet Querol, Amparo Cocolin, Luca Ecological interactions among Saccharomyces cerevisiae strains: insight into the dominance phenomenon |
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This study investigates the behaviour of Saccharomyces cerevisiae strains, in order to obtain insight into the intraspecies competition taking place in mixed populations of this species. Two strains of S. cerevisiae, one dominant and one non-dominant, were labelled and mixed, and individual fermentations were set up to study the transcriptomes of the strains by means of RNA-seq. The results obtained suggest that cell-to-cell contact and aggregation, which are driven by the expression of genes that are associated with the cell surface, are indispensable conditions for the achievement of dominance. Observations on mixed aggregates, made up of cells of both strains, which were detected by means of flow cytometry, have confirmed the transcriptomic data. Furthermore, overexpression of the SSU1 gene, which encodes for a transporter that confers resistance to sulphites, provides an ecological advantage to the dominant strain. A mechanistic model is proposed that sheds light on the dominance phenomenon between different strains of the S. cerevisiae species. The collected data suggest that cell-to-cell contact, together with differential sulphite production and resistance is important in determining the dominance of one strain over another. |
author2 |
Ministerio de Economía y Competitividad (España) |
author_facet |
Ministerio de Economía y Competitividad (España) Pérez Torrado, Roberto Rantsiou, Kalliopi Perrone, Benedetta Navarro Tapia, Elisabet Querol, Amparo Cocolin, Luca |
format |
artículo |
topic_facet |
Saccharomyces cerevisiae Intraspecies competition Dominance Fermentation Aggregation Transcriptoma Sulphites |
author |
Pérez Torrado, Roberto Rantsiou, Kalliopi Perrone, Benedetta Navarro Tapia, Elisabet Querol, Amparo Cocolin, Luca |
author_sort |
Pérez Torrado, Roberto |
title |
Ecological interactions among Saccharomyces cerevisiae strains: insight into the dominance phenomenon |
title_short |
Ecological interactions among Saccharomyces cerevisiae strains: insight into the dominance phenomenon |
title_full |
Ecological interactions among Saccharomyces cerevisiae strains: insight into the dominance phenomenon |
title_fullStr |
Ecological interactions among Saccharomyces cerevisiae strains: insight into the dominance phenomenon |
title_full_unstemmed |
Ecological interactions among Saccharomyces cerevisiae strains: insight into the dominance phenomenon |
title_sort |
ecological interactions among saccharomyces cerevisiae strains: insight into the dominance phenomenon |
publisher |
Nature Publishing Group |
publishDate |
2017-03 |
url |
http://hdl.handle.net/10261/147386 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003359 |
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