Antiviral properties of silver nanoparticles against norovirus surrogates and their efficacy in coated polyhydroxyalkanoates systems
Silver nanoparticles (AgNP) have strong broad-spectrum antimicrobial activity and gained increased attention for the development of AgNP based products, including medical and food applications. Initially, the efficacy of AgNP and silver nitrate (AgNO3) was evaluated for inactivating norovirus surrogates, the feline calicivirus (FCV) and the murine norovirus (MNV). These norovirus surrogates were exposed to AgNO3 and AgNP solutions for 24 h at 25 °C and then analyzed by cell-culture assays. Both AgNP and silver ions significantly decreased FCV and MNV infectivity in a dose-dependent manner between concentrations of 2.1 and 21 mg/L. Furthermore, poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) films were prepared by depositing a coating of thermally post-processed electrospun PHBV18/AgNP fiber mats over compression moulded PHBV3 films. After 24 h exposure at 37 °C and 100% RH, no infectious FCV were recovered when in contact with the AgNP films while MNV titers decreased by 0.86 log. The morphology of the PHBV18 and PHBV18/AgNP fibers studied by SEM showed smooth and continuous fibers in both cases and the EDAX analysis confirmed the homogeneously distribution of AgNP into the coating and onto the PHBV3/PHBV18 layer. This study showed, for the first time, the suitability of the PHBV18/AgNP electrospun coating for antiviral surfaces.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2016-07-26
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Subjects: | Noroviruses, Silver nanoparticles, Active packaging, Polyhydroxyalkanoates, Electrospinning, |
Online Access: | http://hdl.handle.net/10261/143550 http://dx.doi.org/10.13039/501100003329 |
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dig-iata-es-10261-1435502017-07-26T04:30:19Z Antiviral properties of silver nanoparticles against norovirus surrogates and their efficacy in coated polyhydroxyalkanoates systems Castro Mayorga, Jinneth Lorena Randazzo, Walter Fabra, María José Lagarón Cabello, José María Aznar, Rosa Sánchez Moragas, Gloria Ministerio de Economía y Competitividad (España) Colciencias (Colombia) Noroviruses Silver nanoparticles Active packaging Polyhydroxyalkanoates Electrospinning Silver nanoparticles (AgNP) have strong broad-spectrum antimicrobial activity and gained increased attention for the development of AgNP based products, including medical and food applications. Initially, the efficacy of AgNP and silver nitrate (AgNO3) was evaluated for inactivating norovirus surrogates, the feline calicivirus (FCV) and the murine norovirus (MNV). These norovirus surrogates were exposed to AgNO3 and AgNP solutions for 24 h at 25 °C and then analyzed by cell-culture assays. Both AgNP and silver ions significantly decreased FCV and MNV infectivity in a dose-dependent manner between concentrations of 2.1 and 21 mg/L. Furthermore, poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) films were prepared by depositing a coating of thermally post-processed electrospun PHBV18/AgNP fiber mats over compression moulded PHBV3 films. After 24 h exposure at 37 °C and 100% RH, no infectious FCV were recovered when in contact with the AgNP films while MNV titers decreased by 0.86 log. The morphology of the PHBV18 and PHBV18/AgNP fibers studied by SEM showed smooth and continuous fibers in both cases and the EDAX analysis confirmed the homogeneously distribution of AgNP into the coating and onto the PHBV3/PHBV18 layer. This study showed, for the first time, the suitability of the PHBV18/AgNP electrospun coating for antiviral surfaces. This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO) (RYC-2012-09950, RYC-2014-158, AGL2015-63855-C2-1-R and INIA grant RTA2014-00024-C04-03). GS and MJF were supported by the “Ramón y Cajal” Young Investigator from the MINECO (RYC-2012-09950 and RYC-2014-15842). JLC-M was supported by the Administrative Department of Science, Technology and Innovation (Colciencias) of Colombian Government. Peer reviewed 2017-02-07T11:29:13Z 2017-02-07T11:29:13Z 2016-07-26 artículo http://purl.org/coar/resource_type/c_6501 LWT-Food Science and Technology (2017) 79: 503-510 0023-6438 http://hdl.handle.net/10261/143550 10.1016/j.lwt.2017.01.065 http://dx.doi.org/10.13039/501100003329 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-63855-C2-1-R info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTA2014-00024-C04-03 Postprint http://dx.doi.org/10.1016/j.lwt.2017.01.065 Sí open Elsevier |
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Noroviruses Silver nanoparticles Active packaging Polyhydroxyalkanoates Electrospinning Noroviruses Silver nanoparticles Active packaging Polyhydroxyalkanoates Electrospinning |
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Noroviruses Silver nanoparticles Active packaging Polyhydroxyalkanoates Electrospinning Noroviruses Silver nanoparticles Active packaging Polyhydroxyalkanoates Electrospinning Castro Mayorga, Jinneth Lorena Randazzo, Walter Fabra, María José Lagarón Cabello, José María Aznar, Rosa Sánchez Moragas, Gloria Antiviral properties of silver nanoparticles against norovirus surrogates and their efficacy in coated polyhydroxyalkanoates systems |
description |
Silver nanoparticles (AgNP) have strong broad-spectrum antimicrobial activity and gained increased attention for the development of AgNP based products, including medical and food applications. Initially, the efficacy of AgNP and silver nitrate (AgNO3) was evaluated for inactivating norovirus surrogates, the feline calicivirus (FCV) and the murine norovirus (MNV). These norovirus surrogates were exposed to AgNO3 and AgNP solutions for 24 h at 25 °C and then analyzed by cell-culture assays. Both AgNP and silver ions significantly decreased FCV and MNV infectivity in a dose-dependent manner between concentrations of 2.1 and 21 mg/L. Furthermore, poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) films were prepared by depositing a coating of thermally post-processed electrospun PHBV18/AgNP fiber mats over compression moulded PHBV3 films. After 24 h exposure at 37 °C and 100% RH, no infectious FCV were recovered when in contact with the AgNP films while MNV titers decreased by 0.86 log. The morphology of the PHBV18 and PHBV18/AgNP fibers studied by SEM showed smooth and continuous fibers in both cases and the EDAX analysis confirmed the homogeneously distribution of AgNP into the coating and onto the PHBV3/PHBV18 layer. This study showed, for the first time, the suitability of the PHBV18/AgNP electrospun coating for antiviral surfaces. |
author2 |
Ministerio de Economía y Competitividad (España) |
author_facet |
Ministerio de Economía y Competitividad (España) Castro Mayorga, Jinneth Lorena Randazzo, Walter Fabra, María José Lagarón Cabello, José María Aznar, Rosa Sánchez Moragas, Gloria |
format |
artículo |
topic_facet |
Noroviruses Silver nanoparticles Active packaging Polyhydroxyalkanoates Electrospinning |
author |
Castro Mayorga, Jinneth Lorena Randazzo, Walter Fabra, María José Lagarón Cabello, José María Aznar, Rosa Sánchez Moragas, Gloria |
author_sort |
Castro Mayorga, Jinneth Lorena |
title |
Antiviral properties of silver nanoparticles against norovirus surrogates and their efficacy in coated polyhydroxyalkanoates systems |
title_short |
Antiviral properties of silver nanoparticles against norovirus surrogates and their efficacy in coated polyhydroxyalkanoates systems |
title_full |
Antiviral properties of silver nanoparticles against norovirus surrogates and their efficacy in coated polyhydroxyalkanoates systems |
title_fullStr |
Antiviral properties of silver nanoparticles against norovirus surrogates and their efficacy in coated polyhydroxyalkanoates systems |
title_full_unstemmed |
Antiviral properties of silver nanoparticles against norovirus surrogates and their efficacy in coated polyhydroxyalkanoates systems |
title_sort |
antiviral properties of silver nanoparticles against norovirus surrogates and their efficacy in coated polyhydroxyalkanoates systems |
publisher |
Elsevier |
publishDate |
2016-07-26 |
url |
http://hdl.handle.net/10261/143550 http://dx.doi.org/10.13039/501100003329 |
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1777670036558708736 |