Optimisation of a simple and reliable label-free methodology for the relative quantitation of raw pork meat proteins
Recent advances in proteomics have become an indispensable tool for a fast, precise and sensitive analysis of proteins in complex biological samples at both, qualitative and quantitative level. In this study, a label-free quantitative proteomic methodology has been optimised for the relative quantitation of proteins extracted from raw pork meat. So, after the separation of proteins by one-dimensional gel electrophoresis and trypsin digestion, their identification and quantitation have been done using nanoliquid chromatography coupled to a quadrupole/time-of-flight (Q/ToF) mass spectrometer. Relative quantitation has been based on the measurement of mass spectral peak intensities, which have been described that are correlated with protein abundances. The results obtained regarding linearity, robustness, repeatability and accuracy show that this procedure could be used as a fast, simple, and reliable method to quantify changes in protein abundance in meat samples.
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Elsevier
2015-09-01
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| Subjects: | Label-free, Quantitation, Proteins, Proteomic, Peak intensity, Mass spectrometry, Raw pork meat, |
| Online Access: | http://hdl.handle.net/10261/137841 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100003329 |
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dig-iata-es-10261-1378412020-07-30T11:08:46Z Optimisation of a simple and reliable label-free methodology for the relative quantitation of raw pork meat proteins Gallego, Marta Mora, Leticia Aristoy, María Concepción Toldrá Vilardell, Fidel European Commission Consejo Superior de Investigaciones Científicas (España) Ministerio de Economía y Competitividad (España) Label-free Quantitation Proteins Proteomic Peak intensity Mass spectrometry Raw pork meat Recent advances in proteomics have become an indispensable tool for a fast, precise and sensitive analysis of proteins in complex biological samples at both, qualitative and quantitative level. In this study, a label-free quantitative proteomic methodology has been optimised for the relative quantitation of proteins extracted from raw pork meat. So, after the separation of proteins by one-dimensional gel electrophoresis and trypsin digestion, their identification and quantitation have been done using nanoliquid chromatography coupled to a quadrupole/time-of-flight (Q/ToF) mass spectrometer. Relative quantitation has been based on the measurement of mass spectral peak intensities, which have been described that are correlated with protein abundances. The results obtained regarding linearity, robustness, repeatability and accuracy show that this procedure could be used as a fast, simple, and reliable method to quantify changes in protein abundance in meat samples. The research leading to these results received funding from the European Union 7th Framework Programme (FP7/2007-2013) under Grant Agreement 312090 (BACCHUS). This publication reflects only the author views and the Community is not liable for any use made of the information contained therein. JAEDOC-CSIC postdoctoral contract to L.M. is also acknowledged. FPI Scholarship BES-2011-046096 and grant AGL2010-16305 from MINECO (Spain) and FEDER funds are also acknowledged. Peer reviewed 2016-10-07T07:48:14Z 2016-10-07T07:48:14Z 2015-09-01 artículo http://purl.org/coar/resource_type/c_6501 Food Chemistry 182: 74-80 (2015) 0308-8146 http://hdl.handle.net/10261/137841 10.1016/j.foodchem.2015.02.114 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100003329 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/FP7/312090 Postprint http://dx.doi.org/10.1016/j.foodchem.2015.02.114 Sí open Elsevier |
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Label-free Quantitation Proteins Proteomic Peak intensity Mass spectrometry Raw pork meat Label-free Quantitation Proteins Proteomic Peak intensity Mass spectrometry Raw pork meat |
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Label-free Quantitation Proteins Proteomic Peak intensity Mass spectrometry Raw pork meat Label-free Quantitation Proteins Proteomic Peak intensity Mass spectrometry Raw pork meat Gallego, Marta Mora, Leticia Aristoy, María Concepción Toldrá Vilardell, Fidel Optimisation of a simple and reliable label-free methodology for the relative quantitation of raw pork meat proteins |
| description |
Recent advances in proteomics have become an indispensable tool for a fast, precise and sensitive analysis of proteins in complex biological samples at both, qualitative and quantitative level. In this study, a label-free quantitative proteomic methodology has been optimised for the relative quantitation of proteins extracted from raw pork meat. So, after the separation of proteins by one-dimensional gel electrophoresis and trypsin digestion, their identification and quantitation have been done using nanoliquid chromatography coupled to a quadrupole/time-of-flight (Q/ToF) mass spectrometer. Relative quantitation has been based on the measurement of mass spectral peak intensities, which have been described that are correlated with protein abundances. The results obtained regarding linearity, robustness, repeatability and accuracy show that this procedure could be used as a fast, simple, and reliable method to quantify changes in protein abundance in meat samples. |
| author2 |
European Commission |
| author_facet |
European Commission Gallego, Marta Mora, Leticia Aristoy, María Concepción Toldrá Vilardell, Fidel |
| format |
artículo |
| topic_facet |
Label-free Quantitation Proteins Proteomic Peak intensity Mass spectrometry Raw pork meat |
| author |
Gallego, Marta Mora, Leticia Aristoy, María Concepción Toldrá Vilardell, Fidel |
| author_sort |
Gallego, Marta |
| title |
Optimisation of a simple and reliable label-free methodology for the relative quantitation of raw pork meat proteins |
| title_short |
Optimisation of a simple and reliable label-free methodology for the relative quantitation of raw pork meat proteins |
| title_full |
Optimisation of a simple and reliable label-free methodology for the relative quantitation of raw pork meat proteins |
| title_fullStr |
Optimisation of a simple and reliable label-free methodology for the relative quantitation of raw pork meat proteins |
| title_full_unstemmed |
Optimisation of a simple and reliable label-free methodology for the relative quantitation of raw pork meat proteins |
| title_sort |
optimisation of a simple and reliable label-free methodology for the relative quantitation of raw pork meat proteins |
| publisher |
Elsevier |
| publishDate |
2015-09-01 |
| url |
http://hdl.handle.net/10261/137841 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100003329 |
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