Molecular characterisation of the Wx-B1 allelic variants identified in cultivated emmer wheat and comparison with those of durum wheat
Emmer wheat is a neglected crop that could be used in the breeding of modern durum wheat for quality, one important aspect of which is the starch composition that is related to the waxy proteins. A collection of 87 accessions of Spanish emmer wheat was analysed for waxy protein composition by SDS-PAGE. No polymorphism was found for the Wx-A1 gene. However, for the Wx-B1 gene, three alleles were detected, two of them new. The whole gene sequence of these alleles was amplified by PCR in three fragments, which were digested with several endonucleases to determine internal differences in the sequence. These variants were also compared with the Wx alleles present in durum wheat. Differences in size and restriction sites were detected. DNA sequence analysis confirmed that the alleles found in emmer wheat are different from those in durum wheat. The first data suggested that these alleles showed a different influence on the amylose content of these lines. The variation found could be used to enlarge the gene pool of durum and emmer wheat, and design new materials with different amylose content. © 2010 Springer Science+Business Media B.V.
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Kluwer Academic Publishers
2011-10
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Subjects: | Amylose content, Emmer wheat, Genetic polymorphism, Waxy proteins, |
Online Access: | http://hdl.handle.net/10261/89935 |
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dig-ias-es-10261-899352016-02-18T03:00:50Z Molecular characterisation of the Wx-B1 allelic variants identified in cultivated emmer wheat and comparison with those of durum wheat Guzmán, Carlos Caballero, Leonor Álvarez, Juan B. Amylose content Emmer wheat Genetic polymorphism Waxy proteins Emmer wheat is a neglected crop that could be used in the breeding of modern durum wheat for quality, one important aspect of which is the starch composition that is related to the waxy proteins. A collection of 87 accessions of Spanish emmer wheat was analysed for waxy protein composition by SDS-PAGE. No polymorphism was found for the Wx-A1 gene. However, for the Wx-B1 gene, three alleles were detected, two of them new. The whole gene sequence of these alleles was amplified by PCR in three fragments, which were digested with several endonucleases to determine internal differences in the sequence. These variants were also compared with the Wx alleles present in durum wheat. Differences in size and restriction sites were detected. DNA sequence analysis confirmed that the alleles found in emmer wheat are different from those in durum wheat. The first data suggested that these alleles showed a different influence on the amylose content of these lines. The variation found could be used to enlarge the gene pool of durum and emmer wheat, and design new materials with different amylose content. © 2010 Springer Science+Business Media B.V. This research was supported by grant AGL2007-65685-C02-02 from the Spanish Ministry of Science and Innovation and the European Regional Development Fund (FEDER) from the European Union. Peer Reviewed 2014-01-27T09:39:11Z 2014-01-27T09:39:11Z 2011-10 2014-01-27T09:39:11Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1007/s11032-010-9493-2 issn: 1380-3743 e-issn: 1572-9788 Molecular Breeding 28(3): 403-411 (2011) http://hdl.handle.net/10261/89935 10.1007/s11032-010-9493-2 en none Kluwer Academic Publishers |
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Amylose content Emmer wheat Genetic polymorphism Waxy proteins Amylose content Emmer wheat Genetic polymorphism Waxy proteins Guzmán, Carlos Caballero, Leonor Álvarez, Juan B. Molecular characterisation of the Wx-B1 allelic variants identified in cultivated emmer wheat and comparison with those of durum wheat |
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Emmer wheat is a neglected crop that could be used in the breeding of modern durum wheat for quality, one important aspect of which is the starch composition that is related to the waxy proteins. A collection of 87 accessions of Spanish emmer wheat was analysed for waxy protein composition by SDS-PAGE. No polymorphism was found for the Wx-A1 gene. However, for the Wx-B1 gene, three alleles were detected, two of them new. The whole gene sequence of these alleles was amplified by PCR in three fragments, which were digested with several endonucleases to determine internal differences in the sequence. These variants were also compared with the Wx alleles present in durum wheat. Differences in size and restriction sites were detected. DNA sequence analysis confirmed that the alleles found in emmer wheat are different from those in durum wheat. The first data suggested that these alleles showed a different influence on the amylose content of these lines. The variation found could be used to enlarge the gene pool of durum and emmer wheat, and design new materials with different amylose content. © 2010 Springer Science+Business Media B.V. |
format |
artículo |
topic_facet |
Amylose content Emmer wheat Genetic polymorphism Waxy proteins |
author |
Guzmán, Carlos Caballero, Leonor Álvarez, Juan B. |
author_facet |
Guzmán, Carlos Caballero, Leonor Álvarez, Juan B. |
author_sort |
Guzmán, Carlos |
title |
Molecular characterisation of the Wx-B1 allelic variants identified in cultivated emmer wheat and comparison with those of durum wheat |
title_short |
Molecular characterisation of the Wx-B1 allelic variants identified in cultivated emmer wheat and comparison with those of durum wheat |
title_full |
Molecular characterisation of the Wx-B1 allelic variants identified in cultivated emmer wheat and comparison with those of durum wheat |
title_fullStr |
Molecular characterisation of the Wx-B1 allelic variants identified in cultivated emmer wheat and comparison with those of durum wheat |
title_full_unstemmed |
Molecular characterisation of the Wx-B1 allelic variants identified in cultivated emmer wheat and comparison with those of durum wheat |
title_sort |
molecular characterisation of the wx-b1 allelic variants identified in cultivated emmer wheat and comparison with those of durum wheat |
publisher |
Kluwer Academic Publishers |
publishDate |
2011-10 |
url |
http://hdl.handle.net/10261/89935 |
work_keys_str_mv |
AT guzmancarlos molecularcharacterisationofthewxb1allelicvariantsidentifiedincultivatedemmerwheatandcomparisonwiththoseofdurumwheat AT caballeroleonor molecularcharacterisationofthewxb1allelicvariantsidentifiedincultivatedemmerwheatandcomparisonwiththoseofdurumwheat AT alvarezjuanb molecularcharacterisationofthewxb1allelicvariantsidentifiedincultivatedemmerwheatandcomparisonwiththoseofdurumwheat |
_version_ |
1777663000172298240 |