Transferability and polymorphism of barley EST-SSR markers used for phylogenetic analysis in Hordeum chilense

Transferability and polymorphism of barley EST-SSR markers used for phylogenetic analysis in Hordeum chilense

[Background] Hordeum chilense, a native South American diploid wild barley, is a potential source of useful genes for cereal breeding. The use of this wild species to increase genetic variation in cereals will be greatly facilitated by marker-assisted selection. Different economically feasible approaches have been undertaken for this wild species with limited direct agricultural use in a search for suitable and cost-effective markers. The availability of Expressed Sequence Tags (EST) derived microsatellites or simple sequence repeat (SSR) markers, commonly called as EST-SSRs, for barley (Hordeum vulgare) represents a promising source to increase the number of genetic markers available for the H. chilense genome.

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Main Authors: Castillo, Almudena, Budak, Hikmet, Varshney, Rajeev K., Dorado, Gabriel, Graner, Andreas, Hernández Molina, Pilar
Other Authors: Ministerio de Ciencia y Tecnología (España)
Format: artículo biblioteca
Language:English
Published: BioMed Central 2008-09-28
Subjects:Phylogenetic analysis, Hordeum chilense,
Online Access:http://hdl.handle.net/10261/8257
http://dx.doi.org/10.13039/501100006280
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spelling dig-ias-es-10261-82572021-12-28T16:00:50Z Transferability and polymorphism of barley EST-SSR markers used for phylogenetic analysis in Hordeum chilense Castillo, Almudena Budak, Hikmet Varshney, Rajeev K. Dorado, Gabriel Graner, Andreas Hernández Molina, Pilar Ministerio de Ciencia y Tecnología (España) Phylogenetic analysis Hordeum chilense [Background] Hordeum chilense, a native South American diploid wild barley, is a potential source of useful genes for cereal breeding. The use of this wild species to increase genetic variation in cereals will be greatly facilitated by marker-assisted selection. Different economically feasible approaches have been undertaken for this wild species with limited direct agricultural use in a search for suitable and cost-effective markers. The availability of Expressed Sequence Tags (EST) derived microsatellites or simple sequence repeat (SSR) markers, commonly called as EST-SSRs, for barley (Hordeum vulgare) represents a promising source to increase the number of genetic markers available for the H. chilense genome. [Results] All of the 82 barley EST-derived SSR primer pairs tested for transferability to H. chilense amplified products of correct size from this species. Of these 82 barley EST-SSRs, 21 (26%) showed polymorphism among H. chilense lines. Identified polymorphic markers were used to test the transferability and polymorphism in other Poaceae family species with the aim of establishing H. chilense phylogenetic relationships. Triticum aestivum-H. chilense addition lines allowed us to determine the chromosomal localizations of EST-SSR markers and confirm conservation of the linkage group. [Conclusion] From the present study a set of 21 polymorphic EST-SSR markers have been identified to be useful for diversity analysis of H. chilense, related wild barleys like H. murinum, and for wheat marker-assisted introgression breeding. Across-genera transferability of the barley EST-SSR markers has allowed phylogenetic inference within the Triticeae complex. Financial support from the Spanish Ministry of Science and Technology, Projects AGL2003-0720 and AGL2006-12550, is acknowledged. We thank Prof. A. Martin (IAS-CSIC, Spain) and Dr. N. Stein (IPK, Germany) for kindly providing plant material. Technical assistance for the capillary electrophoresis separation of SSR fragments at the SCAI (University of Cordoba, Spain) is acknowledged Peer reviewed 2008-11-06T08:28:10Z 2008-11-06T08:28:10Z 2008-09-28 artículo http://purl.org/coar/resource_type/c_6501 BMC Plant Biology 8: 97 (2008) 1471-2229 http://hdl.handle.net/10261/8257 10.1186/1471-2229-8-97 http://dx.doi.org/10.13039/501100006280 18822176 en Publisher’s version http://dx.doi.org/10.1186/1471-2229-8-97 open 502371 bytes application/pdf BioMed Central
institution IAS ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ias-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IAS España
language English
topic Phylogenetic analysis
Hordeum chilense
Phylogenetic analysis
Hordeum chilense
spellingShingle Phylogenetic analysis
Hordeum chilense
Phylogenetic analysis
Hordeum chilense
Castillo, Almudena
Budak, Hikmet
Varshney, Rajeev K.
Dorado, Gabriel
Graner, Andreas
Hernández Molina, Pilar
Transferability and polymorphism of barley EST-SSR markers used for phylogenetic analysis in Hordeum chilense
description [Background] Hordeum chilense, a native South American diploid wild barley, is a potential source of useful genes for cereal breeding. The use of this wild species to increase genetic variation in cereals will be greatly facilitated by marker-assisted selection. Different economically feasible approaches have been undertaken for this wild species with limited direct agricultural use in a search for suitable and cost-effective markers. The availability of Expressed Sequence Tags (EST) derived microsatellites or simple sequence repeat (SSR) markers, commonly called as EST-SSRs, for barley (Hordeum vulgare) represents a promising source to increase the number of genetic markers available for the H. chilense genome.
author2 Ministerio de Ciencia y Tecnología (España)
author_facet Ministerio de Ciencia y Tecnología (España)
Castillo, Almudena
Budak, Hikmet
Varshney, Rajeev K.
Dorado, Gabriel
Graner, Andreas
Hernández Molina, Pilar
format artículo
topic_facet Phylogenetic analysis
Hordeum chilense
author Castillo, Almudena
Budak, Hikmet
Varshney, Rajeev K.
Dorado, Gabriel
Graner, Andreas
Hernández Molina, Pilar
author_sort Castillo, Almudena
title Transferability and polymorphism of barley EST-SSR markers used for phylogenetic analysis in Hordeum chilense
title_short Transferability and polymorphism of barley EST-SSR markers used for phylogenetic analysis in Hordeum chilense
title_full Transferability and polymorphism of barley EST-SSR markers used for phylogenetic analysis in Hordeum chilense
title_fullStr Transferability and polymorphism of barley EST-SSR markers used for phylogenetic analysis in Hordeum chilense
title_full_unstemmed Transferability and polymorphism of barley EST-SSR markers used for phylogenetic analysis in Hordeum chilense
title_sort transferability and polymorphism of barley est-ssr markers used for phylogenetic analysis in hordeum chilense
publisher BioMed Central
publishDate 2008-09-28
url http://hdl.handle.net/10261/8257
http://dx.doi.org/10.13039/501100006280
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