Identification and Characterization of Resistance to Rust in Lentil and Its Wild Relatives
Lentil rust is a major disease worldwide caused by Uromyces viciae-fabae. In this study, we screened a large germplasm collection of cultivated lentils (Lens culinaris ssp. culinaris) and its wild relatives, both in adult plants in the field with a local rust isolate during 2 seasons and in seedlings under controlled conditions with four fungal isolates of worldwide origin. The main results from our study were the following: (1) a significant number of accessions with resistance based on hypersensitive reaction (reduced Infection Type (IT)) were identified in cultivated lentil and in L. ervoides, L. nigricans and L.c. orientalis. The IT scores showed a clear isolate-specific response suggesting race-specificity, so each fungal isolate might be considered a different race. Resistance was identified against all isolates what might be the basis to develop a standard differential set that should be a priority for rust definition and monitoring. (2) Interestingly, although at lower frequency than in L. ervoides and L. nigricans, the hypersensitive response was also observed within cultivated lentil, with accession 1561 (L.c. culinaris) displaying resistance to the four isolates making this accession a valuable ready-to-use resource for lentil resistance breeding. Resistance to all other rust isolates was also available within L.c. culinaris in an isolate-specific manner. Accession 1308 (L. ervoides) showed resistance against all isolates tested, as well as a reduced number of accessions belonging to other wild Lens species. (3) In addition, our screenings allowed the identification of several accessions with partial resistance (reduced Disease Severity (DS) despite high IT). Adult Plant Resistance resulting in reduced severity in adult plants in the field, despite high susceptibility in seedlings, was more frequently identified in L.c. culinaris, but also in L. nigricans and L.c. orientalis.
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Multidisciplinary Digital Publishing Institute
2023-01-31
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Subjects: | Screening, Lens, Uromyces viciae-fabae, Crop wild, Differentials set, Hypersensitive response, Plant breeding, Quantitative resistance, |
Online Access: | http://hdl.handle.net/10261/349017 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85147864081 |
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dig-ias-es-10261-3490172024-05-15T20:41:41Z Identification and Characterization of Resistance to Rust in Lentil and Its Wild Relatives Barilli, Eleonora Rubiales, Diego Agencia Estatal de Investigación (España) Barilli, Eleonora [0000-0001-6234-6312] Rubiales, Diego [0000-0001-9644-8616] Screening Lens Uromyces viciae-fabae Crop wild Differentials set Hypersensitive response Plant breeding Quantitative resistance Lentil rust is a major disease worldwide caused by Uromyces viciae-fabae. In this study, we screened a large germplasm collection of cultivated lentils (Lens culinaris ssp. culinaris) and its wild relatives, both in adult plants in the field with a local rust isolate during 2 seasons and in seedlings under controlled conditions with four fungal isolates of worldwide origin. The main results from our study were the following: (1) a significant number of accessions with resistance based on hypersensitive reaction (reduced Infection Type (IT)) were identified in cultivated lentil and in L. ervoides, L. nigricans and L.c. orientalis. The IT scores showed a clear isolate-specific response suggesting race-specificity, so each fungal isolate might be considered a different race. Resistance was identified against all isolates what might be the basis to develop a standard differential set that should be a priority for rust definition and monitoring. (2) Interestingly, although at lower frequency than in L. ervoides and L. nigricans, the hypersensitive response was also observed within cultivated lentil, with accession 1561 (L.c. culinaris) displaying resistance to the four isolates making this accession a valuable ready-to-use resource for lentil resistance breeding. Resistance to all other rust isolates was also available within L.c. culinaris in an isolate-specific manner. Accession 1308 (L. ervoides) showed resistance against all isolates tested, as well as a reduced number of accessions belonging to other wild Lens species. (3) In addition, our screenings allowed the identification of several accessions with partial resistance (reduced Disease Severity (DS) despite high IT). Adult Plant Resistance resulting in reduced severity in adult plants in the field, despite high susceptibility in seedlings, was more frequently identified in L.c. culinaris, but also in L. nigricans and L.c. orientalis. This research was funded by the Spanish Agencia Estatal de Investigación (AEI) grant PID2020-114668RB-100. Peer reviewed 2024-03-01T22:27:43Z 2024-03-01T22:27:43Z 2023-01-31 artículo http://purl.org/coar/resource_type/c_6501 Plants 12(3): 626 (2023) http://hdl.handle.net/10261/349017 10.3390/plants12030626 2223-7747 http://dx.doi.org/10.13039/501100011033 36771710 2-s2.0-85147864081 https://api.elsevier.com/content/abstract/scopus_id/85147864081 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-114668RB-I00/ES/MEJORA DE GUISANTES Y ALMORTAS POR RESISTENCIA A ENFERMEDADES Y ADAPTACION A SECANOS MEDITERRANEOS/ Publisher's version Barilli, Eleonora; Rubiales, Diego; 2023; Supplementary Materials. Identification and characterization of resistance to rust in lentil and its wild relatives [Dataset]; Multidisciplinary Digital Publishing Institute; https://www.mdpi.com/article/10.3390/plants12030626/s1 https://doi.org/10.3390/plants12030626 Sí open application/pdf Multidisciplinary Digital Publishing Institute |
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Screening Lens Uromyces viciae-fabae Crop wild Differentials set Hypersensitive response Plant breeding Quantitative resistance Screening Lens Uromyces viciae-fabae Crop wild Differentials set Hypersensitive response Plant breeding Quantitative resistance |
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Screening Lens Uromyces viciae-fabae Crop wild Differentials set Hypersensitive response Plant breeding Quantitative resistance Screening Lens Uromyces viciae-fabae Crop wild Differentials set Hypersensitive response Plant breeding Quantitative resistance Barilli, Eleonora Rubiales, Diego Identification and Characterization of Resistance to Rust in Lentil and Its Wild Relatives |
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Lentil rust is a major disease worldwide caused by Uromyces viciae-fabae. In this study, we screened a large germplasm collection of cultivated lentils (Lens culinaris ssp. culinaris) and its wild relatives, both in adult plants in the field with a local rust isolate during 2 seasons and in seedlings under controlled conditions with four fungal isolates of worldwide origin. The main results from our study were the following: (1) a significant number of accessions with resistance based on hypersensitive reaction (reduced Infection Type (IT)) were identified in cultivated lentil and in L. ervoides, L. nigricans and L.c. orientalis. The IT scores showed a clear isolate-specific response suggesting race-specificity, so each fungal isolate might be considered a different race. Resistance was identified against all isolates what might be the basis to develop a standard differential set that should be a priority for rust definition and monitoring. (2) Interestingly, although at lower frequency than in L. ervoides and L. nigricans, the hypersensitive response was also observed within cultivated lentil, with accession 1561 (L.c. culinaris) displaying resistance to the four isolates making this accession a valuable ready-to-use resource for lentil resistance breeding. Resistance to all other rust isolates was also available within L.c. culinaris in an isolate-specific manner. Accession 1308 (L. ervoides) showed resistance against all isolates tested, as well as a reduced number of accessions belonging to other wild Lens species. (3) In addition, our screenings allowed the identification of several accessions with partial resistance (reduced Disease Severity (DS) despite high IT). Adult Plant Resistance resulting in reduced severity in adult plants in the field, despite high susceptibility in seedlings, was more frequently identified in L.c. culinaris, but also in L. nigricans and L.c. orientalis. |
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Agencia Estatal de Investigación (España) |
author_facet |
Agencia Estatal de Investigación (España) Barilli, Eleonora Rubiales, Diego |
format |
artículo |
topic_facet |
Screening Lens Uromyces viciae-fabae Crop wild Differentials set Hypersensitive response Plant breeding Quantitative resistance |
author |
Barilli, Eleonora Rubiales, Diego |
author_sort |
Barilli, Eleonora |
title |
Identification and Characterization of Resistance to Rust in Lentil and Its Wild Relatives |
title_short |
Identification and Characterization of Resistance to Rust in Lentil and Its Wild Relatives |
title_full |
Identification and Characterization of Resistance to Rust in Lentil and Its Wild Relatives |
title_fullStr |
Identification and Characterization of Resistance to Rust in Lentil and Its Wild Relatives |
title_full_unstemmed |
Identification and Characterization of Resistance to Rust in Lentil and Its Wild Relatives |
title_sort |
identification and characterization of resistance to rust in lentil and its wild relatives |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2023-01-31 |
url |
http://hdl.handle.net/10261/349017 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85147864081 |
work_keys_str_mv |
AT barillieleonora identificationandcharacterizationofresistancetorustinlentilanditswildrelatives AT rubialesdiego identificationandcharacterizationofresistancetorustinlentilanditswildrelatives |
_version_ |
1802820012612780032 |