Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide

Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide

Beyond Xylella, Integrated Management Strategies for Mitigating Xylella fastidiosa Impact in Europe (BeXyl) (Grant Agreement 101060593). Partner/Coordinador principal: Blanca B. Landa del Castillo, Investigadora Científica del Instituto de Agricultura Sostenible (IAS-CSIC).

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Main Authors: Baró, Aina, Saldarelli, Pasquale, Saponari, Maria, Montesinos, Emilio, Montesinos, Laura
Other Authors: European Commission
Format: artículo biblioteca
Language:English
Published: Frontiers Media 2022-12-01
Subjects:Plant infections, Xylella fastidiosa, Bactericidal, Bifunctional peptide, Nicotiana benthamiana, Plant defense elicitor,
Online Access:http://hdl.handle.net/10261/310019
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100002809
https://api.elsevier.com/content/abstract/scopus_id/85144056391
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spelling dig-ias-es-10261-3100192024-10-29T21:38:23Z Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide Baró, Aina Saldarelli, Pasquale Saponari, Maria Montesinos, Emilio Montesinos, Laura European Commission Generalitat de Catalunya Plant infections Xylella fastidiosa Bactericidal Bifunctional peptide Nicotiana benthamiana Plant defense elicitor Beyond Xylella, Integrated Management Strategies for Mitigating Xylella fastidiosa Impact in Europe (BeXyl) (Grant Agreement 101060593). Partner/Coordinador principal: Blanca B. Landa del Castillo, Investigadora Científica del Instituto de Agricultura Sostenible (IAS-CSIC). Transient expression of genes encoding peptides BP134 and BP178 by means of a Potato virus X (PVX) based-vector system, and treatment with synthetic peptides by endotherapy, were evaluated in the control of Xylella fastidiosa infections, in the model plant Nicotiana benthamiana. Transient production of BP178 significantly decreased disease severity compared to PVX and non-treated control (NTC) plants, without adverse effects. Plants treated with synthetic BP134 and BP178 showed consistently lower levels of disease than NTC plants. However, the coinfection with PVX-BP134 and X. fastidiosa caused detrimental effects resulting in plant death. The levels of X. fastidiosa in three zones sampled, upwards and downwards of the inoculation/treatment point, significantly decreased compared to the NTC plants, after the treatment with BP178, but not when BP178 was produced transiently. The effect of treatment and transient production of BP178 in the induction of defense-related genes was also studied. Synthetic BP178 applied by endotherapy induced the expression of ERF1, PR1a, PAL, PALII and WRKY25, while the transient expression of BP178 overexpressed the Cath, Cyc, PR4a, 9-LOX and Endochitinase B genes. Both treatments upregulated the expression of PR1, PR3, PR4 and CycT9299 genes compared to the NTC or PVX plants. It was concluded that the effect of BP178, either by endotherapy or by transient expression, on the control of the X. fastidiosa infections in N. benthamiana, was due in part to the induction of the plant defense system in addition to its bactericidal activity reported in previous studies. However, the protection observed when BP178 was transiently produced seems mainly mediated by the induction of plant defense, because the levels of X. fastidiosa were not significantly affected. This work was supported by grants from the European Union XF-ACTORS (Ref. 727987) and BeXyL (Ref. 101060593) projects. AB was recipient of a research grant from the Secretaria d’Universitats i Recerca, Departament d’Economia i Coneixement, Generalitat de Catalunya (Ref. 2018 FI B00334). Peer reviewed 2023-05-30T09:44:17Z 2023-05-30T09:44:17Z 2022-12-01 artículo http://purl.org/coar/resource_type/c_6501 Frontiers in Plant Science 13: 1061463 (2022) http://hdl.handle.net/10261/310019 10.3389/fpls.2022.1061463 1664-462X http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100002809 36531347 2-s2.0-85144056391 https://api.elsevier.com/content/abstract/scopus_id/85144056391 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/HE/101060593 info:eu-repo/grantAgreement/EC/H2020/727987 Publisher's version https://doi.org/10.3389/fpls.2022.1061463 No open application/pdf Frontiers Media
institution IAS ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ias-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IAS España
language English
topic Plant infections
Xylella fastidiosa
Bactericidal
Bifunctional peptide
Nicotiana benthamiana
Plant defense elicitor
Plant infections
Xylella fastidiosa
Bactericidal
Bifunctional peptide
Nicotiana benthamiana
Plant defense elicitor
spellingShingle Plant infections
Xylella fastidiosa
Bactericidal
Bifunctional peptide
Nicotiana benthamiana
Plant defense elicitor
Plant infections
Xylella fastidiosa
Bactericidal
Bifunctional peptide
Nicotiana benthamiana
Plant defense elicitor
Baró, Aina
Saldarelli, Pasquale
Saponari, Maria
Montesinos, Emilio
Montesinos, Laura
Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide
description Beyond Xylella, Integrated Management Strategies for Mitigating Xylella fastidiosa Impact in Europe (BeXyl) (Grant Agreement 101060593). Partner/Coordinador principal: Blanca B. Landa del Castillo, Investigadora Científica del Instituto de Agricultura Sostenible (IAS-CSIC).
author2 European Commission
author_facet European Commission
Baró, Aina
Saldarelli, Pasquale
Saponari, Maria
Montesinos, Emilio
Montesinos, Laura
format artículo
topic_facet Plant infections
Xylella fastidiosa
Bactericidal
Bifunctional peptide
Nicotiana benthamiana
Plant defense elicitor
author Baró, Aina
Saldarelli, Pasquale
Saponari, Maria
Montesinos, Emilio
Montesinos, Laura
author_sort Baró, Aina
title Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide
title_short Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide
title_full Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide
title_fullStr Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide
title_full_unstemmed Nicotiana benthamiana as a model plant host for Xylella fastidiosa: Control of infections by transient expression and endotherapy with a bifunctional peptide
title_sort nicotiana benthamiana as a model plant host for xylella fastidiosa: control of infections by transient expression and endotherapy with a bifunctional peptide
publisher Frontiers Media
publishDate 2022-12-01
url http://hdl.handle.net/10261/310019
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100002809
https://api.elsevier.com/content/abstract/scopus_id/85144056391
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