Morphological and Molecular Characterization of Ottolenchus isfahanicus n. sp. (Tylenchomorpha: Tylenchoidea) from Rhizosphere of Gramineous Plants in Isfahan Province, Iran
A new species from the family Tylenchidae was recovered in the grasslands of Isfahan province, Iran, and is herein described based on morphological and molecular data. Ottolenchus isfahanicus n. sp. is mainly characterized by having a faintly annulated cuticle; elongated, slightly sigmoid amphidial apertures developed metacorpus with distinct valve under LM; vulva located at 69.4-72.3% of body length; large spermatheca about 2.75 times of corresponding body width; and elongated conoid tail with broadly rounded tip. SEM observations showed that the lip region is smooth; the amphidial apertures are elongated, slightly sigmoid slits; and the lateral field is a simple band. It is further characterized by 477-to-515-μm-long females with delicate 5.7-to-6.9-μm-long stylets with small, slightly posteriorly sloping knobs, as well as functional males, in the population. The new species closely resembles O. facultativus, but is separated from it based on morphological and molecular data. It was further morphologically compared with O. discrepans, O. fungivorus, and O. sinipersici. The phylogenetic relationships of the new species with other relevant genera and species were reconstructed using near-full-length sequences of small subunit and D2-D3 expansion segments of large subunit (SSU and LSU D2-D3). In the inferred SSU phylogeny, the newly generated sequence of Ottolenchus isfahanicus n. sp. formed a clade with two sequences of O. sinipersici and sequences assigned to O. facultativus and O. fungivorus. In the inferred LSU phylogeny, the three newly generated sequences of the new species and LSU sequences of O. sinipersici and O. discrepans formed a clade.
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Language: | English |
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2023-02
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Subjects: | Taxonomy, New species, Ottolenchus facultativus, SSU rDNA, Phylogeny, |
Online Access: | http://hdl.handle.net/10261/307143 |
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dig-ias-es-10261-3071432023-04-24T06:35:58Z Morphological and Molecular Characterization of Ottolenchus isfahanicus n. sp. (Tylenchomorpha: Tylenchoidea) from Rhizosphere of Gramineous Plants in Isfahan Province, Iran Mahboubi, Zeinab Keramat, Mojtaba Pourjam, Ebrahim Atighi, Mohammad Reza Clavero-Camacho, Ilenia Abolafia, Joaquín Castillo, Pablo Pedram, Majid Universidad de Jaén Taxonomy New species Ottolenchus facultativus SSU rDNA Phylogeny A new species from the family Tylenchidae was recovered in the grasslands of Isfahan province, Iran, and is herein described based on morphological and molecular data. Ottolenchus isfahanicus n. sp. is mainly characterized by having a faintly annulated cuticle; elongated, slightly sigmoid amphidial apertures developed metacorpus with distinct valve under LM; vulva located at 69.4-72.3% of body length; large spermatheca about 2.75 times of corresponding body width; and elongated conoid tail with broadly rounded tip. SEM observations showed that the lip region is smooth; the amphidial apertures are elongated, slightly sigmoid slits; and the lateral field is a simple band. It is further characterized by 477-to-515-μm-long females with delicate 5.7-to-6.9-μm-long stylets with small, slightly posteriorly sloping knobs, as well as functional males, in the population. The new species closely resembles O. facultativus, but is separated from it based on morphological and molecular data. It was further morphologically compared with O. discrepans, O. fungivorus, and O. sinipersici. The phylogenetic relationships of the new species with other relevant genera and species were reconstructed using near-full-length sequences of small subunit and D2-D3 expansion segments of large subunit (SSU and LSU D2-D3). In the inferred SSU phylogeny, the newly generated sequence of Ottolenchus isfahanicus n. sp. formed a clade with two sequences of O. sinipersici and sequences assigned to O. facultativus and O. fungivorus. In the inferred LSU phylogeny, the three newly generated sequences of the new species and LSU sequences of O. sinipersici and O. discrepans formed a clade. The financial support of Tarbiat Modares University, Iran, and University of Jaén, Spain, through the research program “PAIUJA 2021/2022: EI_RNM02_2021” are appreciated. SEM pictures were obtained with the assistance of technical staff (Amparo Martínez-Morales) and equipment of “Centro de Instrumentación Científico-Técnica (CICT)” from University of Jaén. Peer reviewed 2023-04-24T06:35:58Z 2023-04-24T06:35:58Z 2023-02 artículo Journal of Nematology 55(1): e2023-1 (2023) 0022-300X http://hdl.handle.net/10261/307143 10.2478/jofnem-2023-0011 2640-396X en Publisher's version https://doi.org/10.2478/jofnem-2023-0011 Sí open application/pdf Sciendo |
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Taxonomy New species Ottolenchus facultativus SSU rDNA Phylogeny Taxonomy New species Ottolenchus facultativus SSU rDNA Phylogeny |
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Taxonomy New species Ottolenchus facultativus SSU rDNA Phylogeny Taxonomy New species Ottolenchus facultativus SSU rDNA Phylogeny Mahboubi, Zeinab Keramat, Mojtaba Pourjam, Ebrahim Atighi, Mohammad Reza Clavero-Camacho, Ilenia Abolafia, Joaquín Castillo, Pablo Pedram, Majid Morphological and Molecular Characterization of Ottolenchus isfahanicus n. sp. (Tylenchomorpha: Tylenchoidea) from Rhizosphere of Gramineous Plants in Isfahan Province, Iran |
description |
A new species from the family Tylenchidae was recovered in the grasslands of Isfahan province, Iran, and is herein described based on morphological and molecular data. Ottolenchus isfahanicus n. sp. is mainly characterized by having a faintly annulated cuticle; elongated, slightly sigmoid amphidial apertures developed metacorpus with distinct valve under LM; vulva located at 69.4-72.3% of body length; large spermatheca about 2.75 times of corresponding body width; and elongated conoid tail with broadly rounded tip. SEM observations showed that the lip region is smooth; the amphidial apertures are elongated, slightly sigmoid slits; and the lateral field is a simple band. It is further characterized by 477-to-515-μm-long females with delicate 5.7-to-6.9-μm-long stylets with small, slightly posteriorly sloping knobs, as well as functional males, in the population. The new species closely resembles O. facultativus, but is separated from it based on morphological and molecular data. It was further morphologically compared with O. discrepans, O. fungivorus, and O. sinipersici. The phylogenetic relationships of the new species with other relevant genera and species were reconstructed using near-full-length sequences of small subunit and D2-D3 expansion segments of large subunit (SSU and LSU D2-D3). In the inferred SSU phylogeny, the newly generated sequence of Ottolenchus isfahanicus n. sp. formed a clade with two sequences of O. sinipersici and sequences assigned to O. facultativus and O. fungivorus. In the inferred LSU phylogeny, the three newly generated sequences of the new species and LSU sequences of O. sinipersici and O. discrepans formed a clade. |
author2 |
Universidad de Jaén |
author_facet |
Universidad de Jaén Mahboubi, Zeinab Keramat, Mojtaba Pourjam, Ebrahim Atighi, Mohammad Reza Clavero-Camacho, Ilenia Abolafia, Joaquín Castillo, Pablo Pedram, Majid |
format |
artículo |
topic_facet |
Taxonomy New species Ottolenchus facultativus SSU rDNA Phylogeny |
author |
Mahboubi, Zeinab Keramat, Mojtaba Pourjam, Ebrahim Atighi, Mohammad Reza Clavero-Camacho, Ilenia Abolafia, Joaquín Castillo, Pablo Pedram, Majid |
author_sort |
Mahboubi, Zeinab |
title |
Morphological and Molecular Characterization of Ottolenchus isfahanicus n. sp. (Tylenchomorpha: Tylenchoidea) from Rhizosphere of Gramineous Plants in Isfahan Province, Iran |
title_short |
Morphological and Molecular Characterization of Ottolenchus isfahanicus n. sp. (Tylenchomorpha: Tylenchoidea) from Rhizosphere of Gramineous Plants in Isfahan Province, Iran |
title_full |
Morphological and Molecular Characterization of Ottolenchus isfahanicus n. sp. (Tylenchomorpha: Tylenchoidea) from Rhizosphere of Gramineous Plants in Isfahan Province, Iran |
title_fullStr |
Morphological and Molecular Characterization of Ottolenchus isfahanicus n. sp. (Tylenchomorpha: Tylenchoidea) from Rhizosphere of Gramineous Plants in Isfahan Province, Iran |
title_full_unstemmed |
Morphological and Molecular Characterization of Ottolenchus isfahanicus n. sp. (Tylenchomorpha: Tylenchoidea) from Rhizosphere of Gramineous Plants in Isfahan Province, Iran |
title_sort |
morphological and molecular characterization of ottolenchus isfahanicus n. sp. (tylenchomorpha: tylenchoidea) from rhizosphere of gramineous plants in isfahan province, iran |
publisher |
Sciendo |
publishDate |
2023-02 |
url |
http://hdl.handle.net/10261/307143 |
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