Verticillium wilt resistant and susceptible olive cultivars express a very different basal set of genes in roots
[Background] Olive orchards are threatened by a wide range of pathogens. Of these, Verticillium dahliae has been in the spotlight for its high incidence, the difficulty to control it and the few cultivars that has increased tolerance to the pathogen. Disease resistance not only depends on detection of pathogen invasion and induction of responses by the plant, but also on barriers to avoid the invasion and active resistance mechanisms constitutively expressed in the absence of the pathogen. In a previous work we found that two healthy non-infected plants from cultivars that differ in V. dahliae resistance such as ‘Frantoio’ (resistant) and ‘Picual’ (susceptible) had a different root morphology and gene expression pattern. In this work, we have addressed the issue of basal differences in the roots between Resistant and Susceptible cultivars.
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BioMed Central
2021-04-01
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Subjects: | RNA-Seq, Olea europaea L., Roots, Transcriptomes, Verticillium dahliae, |
Online Access: | http://hdl.handle.net/10261/268177 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100010198 http://dx.doi.org/10.13039/501100011033 |
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dig-ias-es-10261-2681772022-04-29T02:20:46Z Verticillium wilt resistant and susceptible olive cultivars express a very different basal set of genes in roots Ramírez-Tejero, J. A. Jiménez-Ruiz, Jaime Serrano, Alicia Belaj, Angjelina León, Lorenzo Mercado-Blanco, Jesús Luque, Fernando Ministerio de Economía, Industria y Competitividad (España) Agencia Estatal de Investigación (España) European Commission RNA-Seq Olea europaea L. Roots Transcriptomes Verticillium dahliae [Background] Olive orchards are threatened by a wide range of pathogens. Of these, Verticillium dahliae has been in the spotlight for its high incidence, the difficulty to control it and the few cultivars that has increased tolerance to the pathogen. Disease resistance not only depends on detection of pathogen invasion and induction of responses by the plant, but also on barriers to avoid the invasion and active resistance mechanisms constitutively expressed in the absence of the pathogen. In a previous work we found that two healthy non-infected plants from cultivars that differ in V. dahliae resistance such as ‘Frantoio’ (resistant) and ‘Picual’ (susceptible) had a different root morphology and gene expression pattern. In this work, we have addressed the issue of basal differences in the roots between Resistant and Susceptible cultivars. [Results] The gene expression pattern of roots from 29 olive cultivars with different degree of resistance/susceptibility to V. dahliae was analyzed by RNA-Seq. However, only the Highly Resistant and Extremely Susceptible cultivars showed significant differences in gene expression among various groups of cultivars. A set of 421 genes showing an inverse differential expression level between the Highly Resistant to Extremely Susceptible cultivars was found and analyzed. The main differences involved higher expression of a series of transcription factors and genes involved in processes of molecules importation to nucleus, plant defense genes and lower expression of root growth and development genes in Highly Resistant cultivars, while a reverse pattern in Moderately Susceptible and more pronounced in Extremely Susceptible cultivars were observed. [Conclusion] According to the different gene expression patterns, it seems that the roots of the Extremely Susceptible cultivars focus more on growth and development, while some other functions, such as defense against pathogens, have a higher expression level in roots of Highly Resistant cultivars. Therefore, it seems that there are constitutive differences in the roots between Resistant and Susceptible cultivars, and that susceptible roots seem to provide a more suitable environment for the pathogen than the resistant ones. This research was funded by the Spanish Ministry of Economy, Industry and Competitiveness (MINECO)/Spanish State Research Agency (AEI) and co-financed by the European Regional Development Fund, ERDF, grant number AGL2016–75729-C2–2-R. Peer reviewed 2022-04-28T12:30:03Z 2022-04-28T12:30:03Z 2021-04-01 2022-04-28T12:30:03Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1186/s12864-021-07545-x e-issn: 1471-2164 BMC Genomics 22: 229 (2021) http://hdl.handle.net/10261/268177 10.1186/s12864-021-07545-x http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100010198 http://dx.doi.org/10.13039/501100011033 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO//AGL2016–75729-C2–2-R Publisher's version http://dx.doi.org/10.1186/s12864-021-07545-x Sí open BioMed Central |
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RNA-Seq Olea europaea L. Roots Transcriptomes Verticillium dahliae RNA-Seq Olea europaea L. Roots Transcriptomes Verticillium dahliae |
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RNA-Seq Olea europaea L. Roots Transcriptomes Verticillium dahliae RNA-Seq Olea europaea L. Roots Transcriptomes Verticillium dahliae Ramírez-Tejero, J. A. Jiménez-Ruiz, Jaime Serrano, Alicia Belaj, Angjelina León, Lorenzo Mercado-Blanco, Jesús Luque, Fernando Verticillium wilt resistant and susceptible olive cultivars express a very different basal set of genes in roots |
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[Background] Olive orchards are threatened by a wide range of pathogens. Of these, Verticillium dahliae has been in the spotlight for its high incidence, the difficulty to control it and the few cultivars that has increased tolerance to the pathogen. Disease resistance not only depends on detection of pathogen invasion and induction of responses by the plant, but also on barriers to avoid the invasion and active resistance mechanisms constitutively expressed in the absence of the pathogen. In a previous work we found that two healthy non-infected plants from cultivars that differ in V. dahliae resistance such as ‘Frantoio’ (resistant) and ‘Picual’ (susceptible) had a different root morphology and gene expression pattern. In this work, we have addressed the issue of basal differences in the roots between Resistant and Susceptible cultivars. |
author2 |
Ministerio de Economía, Industria y Competitividad (España) |
author_facet |
Ministerio de Economía, Industria y Competitividad (España) Ramírez-Tejero, J. A. Jiménez-Ruiz, Jaime Serrano, Alicia Belaj, Angjelina León, Lorenzo Mercado-Blanco, Jesús Luque, Fernando |
format |
artículo |
topic_facet |
RNA-Seq Olea europaea L. Roots Transcriptomes Verticillium dahliae |
author |
Ramírez-Tejero, J. A. Jiménez-Ruiz, Jaime Serrano, Alicia Belaj, Angjelina León, Lorenzo Mercado-Blanco, Jesús Luque, Fernando |
author_sort |
Ramírez-Tejero, J. A. |
title |
Verticillium wilt resistant and susceptible olive cultivars express a very different basal set of genes in roots |
title_short |
Verticillium wilt resistant and susceptible olive cultivars express a very different basal set of genes in roots |
title_full |
Verticillium wilt resistant and susceptible olive cultivars express a very different basal set of genes in roots |
title_fullStr |
Verticillium wilt resistant and susceptible olive cultivars express a very different basal set of genes in roots |
title_full_unstemmed |
Verticillium wilt resistant and susceptible olive cultivars express a very different basal set of genes in roots |
title_sort |
verticillium wilt resistant and susceptible olive cultivars express a very different basal set of genes in roots |
publisher |
BioMed Central |
publishDate |
2021-04-01 |
url |
http://hdl.handle.net/10261/268177 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100010198 http://dx.doi.org/10.13039/501100011033 |
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