A moderate level of hypovirulence conferred by a hypovirus in the avocado white root rot fungus, Rosellinia necatrix

A moderate level of hypovirulence conferred by a hypovirus in the avocado white root rot fungus, Rosellinia necatrix

Two isolates of Rosellinia necatrix (Rn118-8 and Rn480) have previously obtained from diseased avocado trees in commercial orchards of the coastal area in southern Spain. Rn118-8 and Rn480 have weak virulence on avocado plants, and are infected by R. necatrix hypovirus 2 (RnHV2). In this work, the possible biological effects of the hypovirus on R. necatrix were tested. First, RnHV2 was transmitted from each of Rn118-8 and Rn480 to a highly virulent, RnHV2-free isolate of R. necatrix (Rn400) through hyphal anastomosis, using zinc compounds which attenuate the mycelial incompatibility reactions and allow for horizontal virus transfer between vegetatively incompatible fungal strains. Next, we carried out an analysis of growth rate in vitro and a virulence test of these newly infected strains in avocado plants. We obtained five strains of Rn400 infected by RnHV2 after horizontal transmission, and showed some of them to have lower colony growth in vitro and lower virulence on avocado plants compared with virus-free Rn400. These results suggest that R. necatrix isolates infected by RnHV2 could be used as novel virocontrol agents to combat avocado white root rot.

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Bibliographic Details
Main Authors: Arjona-López, J. M., Telengech, Paul, Suzuki, Nobuhiro, López Herrera, Carlos
Other Authors: Ministerio de Economía y Competitividad (España)
Format: artículo biblioteca
Published: Elsevier 2021-01
Subjects:Anastomosis, Mycovirus transmission, Persea americana, Virocontrol, Virulence,
Online Access:http://hdl.handle.net/10261/267727
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100001700
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spelling dig-ias-es-10261-2677272022-04-26T06:44:26Z A moderate level of hypovirulence conferred by a hypovirus in the avocado white root rot fungus, Rosellinia necatrix Arjona-López, J. M. Telengech, Paul Suzuki, Nobuhiro López Herrera, Carlos Ministerio de Economía y Competitividad (España) European Commission Ministry of Education, Culture, Sports, Science and Technology (Japan) Anastomosis Mycovirus transmission Persea americana Virocontrol Virulence Two isolates of Rosellinia necatrix (Rn118-8 and Rn480) have previously obtained from diseased avocado trees in commercial orchards of the coastal area in southern Spain. Rn118-8 and Rn480 have weak virulence on avocado plants, and are infected by R. necatrix hypovirus 2 (RnHV2). In this work, the possible biological effects of the hypovirus on R. necatrix were tested. First, RnHV2 was transmitted from each of Rn118-8 and Rn480 to a highly virulent, RnHV2-free isolate of R. necatrix (Rn400) through hyphal anastomosis, using zinc compounds which attenuate the mycelial incompatibility reactions and allow for horizontal virus transfer between vegetatively incompatible fungal strains. Next, we carried out an analysis of growth rate in vitro and a virulence test of these newly infected strains in avocado plants. We obtained five strains of Rn400 infected by RnHV2 after horizontal transmission, and showed some of them to have lower colony growth in vitro and lower virulence on avocado plants compared with virus-free Rn400. These results suggest that R. necatrix isolates infected by RnHV2 could be used as novel virocontrol agents to combat avocado white root rot. This study was supported in part by the Plan Nacional I + D + I Ministerio de Economía y Competitividad (AGL 2014-52518-C2-2-R) Spain and co-financed by FEDER funds (EU) and by Yomogi Inc. and Grants-in-Aid for Scientific Research (A) and Scientific Research on Innovative Areas from the Japanese Ministry of Education, Culture, Sports, Science and Technology (17H01463 and 16H06436, 16H06429 and 16K21723 to N.S.). Peer reviewed 2022-04-25T09:20:03Z 2022-04-25T09:20:03Z 2021-01 2022-04-25T09:20:03Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.funbio.2020.10.007 issn: 1878-6146 Fungal Biology 125(1): 69-76 (2021) http://hdl.handle.net/10261/267727 10.1016/j.funbio.2020.10.007 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100001700 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO//AGL2014-52518-C2-2-R/ES/PATOGENICIDAD DE ROSELLINIA NECATRIX VERSUS MYCOVIRUS. BIOCONTOL (VIROCONTROL, HONGOS ANTAGONISTAS). COMBINACION DE METODOS BIOLOGICOS Y QUIMICOS DE CONTROL DE PB DEL AGUACATE/ http://dx.doi.org/10.1016/j.funbio.2020.10.007 Sí none Elsevier
institution IAS ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ias-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IAS España
topic Anastomosis
Mycovirus transmission
Persea americana
Virocontrol
Virulence
Anastomosis
Mycovirus transmission
Persea americana
Virocontrol
Virulence
spellingShingle Anastomosis
Mycovirus transmission
Persea americana
Virocontrol
Virulence
Anastomosis
Mycovirus transmission
Persea americana
Virocontrol
Virulence
Arjona-López, J. M.
Telengech, Paul
Suzuki, Nobuhiro
López Herrera, Carlos
A moderate level of hypovirulence conferred by a hypovirus in the avocado white root rot fungus, Rosellinia necatrix
description Two isolates of Rosellinia necatrix (Rn118-8 and Rn480) have previously obtained from diseased avocado trees in commercial orchards of the coastal area in southern Spain. Rn118-8 and Rn480 have weak virulence on avocado plants, and are infected by R. necatrix hypovirus 2 (RnHV2). In this work, the possible biological effects of the hypovirus on R. necatrix were tested. First, RnHV2 was transmitted from each of Rn118-8 and Rn480 to a highly virulent, RnHV2-free isolate of R. necatrix (Rn400) through hyphal anastomosis, using zinc compounds which attenuate the mycelial incompatibility reactions and allow for horizontal virus transfer between vegetatively incompatible fungal strains. Next, we carried out an analysis of growth rate in vitro and a virulence test of these newly infected strains in avocado plants. We obtained five strains of Rn400 infected by RnHV2 after horizontal transmission, and showed some of them to have lower colony growth in vitro and lower virulence on avocado plants compared with virus-free Rn400. These results suggest that R. necatrix isolates infected by RnHV2 could be used as novel virocontrol agents to combat avocado white root rot.
author2 Ministerio de Economía y Competitividad (España)
author_facet Ministerio de Economía y Competitividad (España)
Arjona-López, J. M.
Telengech, Paul
Suzuki, Nobuhiro
López Herrera, Carlos
format artículo
topic_facet Anastomosis
Mycovirus transmission
Persea americana
Virocontrol
Virulence
author Arjona-López, J. M.
Telengech, Paul
Suzuki, Nobuhiro
López Herrera, Carlos
author_sort Arjona-López, J. M.
title A moderate level of hypovirulence conferred by a hypovirus in the avocado white root rot fungus, Rosellinia necatrix
title_short A moderate level of hypovirulence conferred by a hypovirus in the avocado white root rot fungus, Rosellinia necatrix
title_full A moderate level of hypovirulence conferred by a hypovirus in the avocado white root rot fungus, Rosellinia necatrix
title_fullStr A moderate level of hypovirulence conferred by a hypovirus in the avocado white root rot fungus, Rosellinia necatrix
title_full_unstemmed A moderate level of hypovirulence conferred by a hypovirus in the avocado white root rot fungus, Rosellinia necatrix
title_sort moderate level of hypovirulence conferred by a hypovirus in the avocado white root rot fungus, rosellinia necatrix
publisher Elsevier
publishDate 2021-01
url http://hdl.handle.net/10261/267727
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/501100001700
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