Culture and metagenomic approaches for the identification of olive xylem microbial communities as a biological control tool to cope against Xylella fastidiosa infection

Culture and metagenomic approaches for the identification of olive xylem microbial communities as a biological control tool to cope against Xylella fastidiosa infection

Trabajo presentado en la 3rd European Conference on Xylella fastidiosa (Building knowledge, protecting plant health), celebrada online el 29 y 30 de abril de 2021.

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Main Authors: Anguita-Maeso, Manuel, Navas Cortés, Juan Antonio, Coletta-Filho, Helvecio D., Landa, Blanca B.
Other Authors: European Commission
Format: póster de congreso biblioteca
Published: 2021-04
Subjects:Plant health, Xylella,
Online Access:http://hdl.handle.net/10261/267095
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003329
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spelling dig-ias-es-10261-2670952022-04-19T01:50:45Z Culture and metagenomic approaches for the identification of olive xylem microbial communities as a biological control tool to cope against Xylella fastidiosa infection Anguita-Maeso, Manuel Navas Cortés, Juan Antonio Coletta-Filho, Helvecio D. Landa, Blanca B. European Commission Ministerio de Economía y Competitividad (España) Fundación Carolina Plant health Xylella Trabajo presentado en la 3rd European Conference on Xylella fastidiosa (Building knowledge, protecting plant health), celebrada online el 29 y 30 de abril de 2021. The xylem-inhabiting plant pathogenic bacterium Xylella fastidiosa (Xf) represents one of the major phytopathological threats to olive crop worldwide, due to its devastating effects on agricultural yields losses and high tree mortality that causes profound socioeconomic and environmental impacts. Endophytes play an essential role on plant growth and its physiological status, but they can also act as an innate natural defense to cope against infection by xylem-inhabiting pathogenic organisms. Today, vast majority of microorganisms residing in olive xylem are unknown; therefore this work pursues the characterization of the olive microbiome through culture-dependent and independent (NGS) techniques as a tool for identifying potential biological control agents for this pathogen. Hence, four cultivated olive genotypes (Arbequina, Arbosana, Koroneiki and Grappolo) located in Sao Paulo state (Brazil) showing visual Xf symptoms or asymptomatic-non-infected were selected. Xf infection was verified by qPCR. For the culture-dependent approach, chips extracts of xylem tissue from branches and roots were plated in two solid media (R2A and R2A supplemented with plant extract). For culture independent approach, total DNA extracted from xylem tissue was analyzed by metagenomic analysis of 16S and ITS region to characterize the xylem-inhabiting bacterial and fungal communities. Preliminary culture results indicated differences in the frequency of microbial communities depending on the olive genotype and the type of plant material analyzed, as well as, the presence or absence of Xf symptoms on the sampled trees that correlated with Xf infection. These results will help to expand our knowledge on the olive xylem microbiome community composition and understand its driving factors when Xf infection occurs and more importantly to identify xylem-inhabiting microorganisms with potential to combat this harmful bacterium. Study supported by Projects 727987 XF-ACTORS (EU-H2020) and AGL2016-75606-R (MEIC Spain and FEDER-EU) and SEGIB – Carolina Foundation. 2022-04-18T12:40:12Z 2022-04-18T12:40:12Z 2021-04 2022-04-18T12:40:12Z póster de congreso http://purl.org/coar/resource_type/c_6670 doi: 10.5281/zenodo.4671712 3rd European Conference on Xylella fastidiosa (2021) http://hdl.handle.net/10261/267095 10.5281/zenodo.4671712 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/727987 info:eu-repo/grantAgreement/MINECO//AGL2016-75606-R https://doi.org/10.5281/zenodo.4671712 Sí open
institution IAS ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ias-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IAS España
topic Plant health
Xylella
Plant health
Xylella
spellingShingle Plant health
Xylella
Plant health
Xylella
Anguita-Maeso, Manuel
Navas Cortés, Juan Antonio
Coletta-Filho, Helvecio D.
Landa, Blanca B.
Culture and metagenomic approaches for the identification of olive xylem microbial communities as a biological control tool to cope against Xylella fastidiosa infection
description Trabajo presentado en la 3rd European Conference on Xylella fastidiosa (Building knowledge, protecting plant health), celebrada online el 29 y 30 de abril de 2021.
author2 European Commission
author_facet European Commission
Anguita-Maeso, Manuel
Navas Cortés, Juan Antonio
Coletta-Filho, Helvecio D.
Landa, Blanca B.
format póster de congreso
topic_facet Plant health
Xylella
author Anguita-Maeso, Manuel
Navas Cortés, Juan Antonio
Coletta-Filho, Helvecio D.
Landa, Blanca B.
author_sort Anguita-Maeso, Manuel
title Culture and metagenomic approaches for the identification of olive xylem microbial communities as a biological control tool to cope against Xylella fastidiosa infection
title_short Culture and metagenomic approaches for the identification of olive xylem microbial communities as a biological control tool to cope against Xylella fastidiosa infection
title_full Culture and metagenomic approaches for the identification of olive xylem microbial communities as a biological control tool to cope against Xylella fastidiosa infection
title_fullStr Culture and metagenomic approaches for the identification of olive xylem microbial communities as a biological control tool to cope against Xylella fastidiosa infection
title_full_unstemmed Culture and metagenomic approaches for the identification of olive xylem microbial communities as a biological control tool to cope against Xylella fastidiosa infection
title_sort culture and metagenomic approaches for the identification of olive xylem microbial communities as a biological control tool to cope against xylella fastidiosa infection
publishDate 2021-04
url http://hdl.handle.net/10261/267095
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100003329
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AT colettafilhohelveciod cultureandmetagenomicapproachesfortheidentificationofolivexylemmicrobialcommunitiesasabiologicalcontroltooltocopeagainstxylellafastidiosainfection
AT landablancab cultureandmetagenomicapproachesfortheidentificationofolivexylemmicrobialcommunitiesasabiologicalcontroltooltocopeagainstxylellafastidiosainfection
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