Pathogenicity and systemic colonization of Spanish strains of Xylella fastidiosa subsp. multiplex and pauca on olive under controlled conditions
Trabajo presentado en la 3rd European Conference on Xylella fastidiosa (Building knowledge, protecting plant health), celebrada online el 29 y 30 de abril de 2021.
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2021-04
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| Subjects: | Plant health, Xylella, |
| Online Access: | http://hdl.handle.net/10261/267093 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 |
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Plant health Xylella Plant health Xylella Román Ecija, Miguel Olivares-García, Concepción Domínguez-Calero, Cristina Navas Cortés, Juan Antonio Landa, Blanca B. Pathogenicity and systemic colonization of Spanish strains of Xylella fastidiosa subsp. multiplex and pauca on olive under controlled conditions |
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Trabajo presentado en la 3rd European Conference on Xylella fastidiosa (Building knowledge, protecting plant health), celebrada online el 29 y 30 de abril de 2021. |
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European Commission |
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European Commission Román Ecija, Miguel Olivares-García, Concepción Domínguez-Calero, Cristina Navas Cortés, Juan Antonio Landa, Blanca B. |
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póster de congreso |
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Plant health Xylella |
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Román Ecija, Miguel Olivares-García, Concepción Domínguez-Calero, Cristina Navas Cortés, Juan Antonio Landa, Blanca B. |
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Román Ecija, Miguel |
| title |
Pathogenicity and systemic colonization of Spanish strains of Xylella fastidiosa subsp. multiplex and pauca on olive under controlled conditions |
| title_short |
Pathogenicity and systemic colonization of Spanish strains of Xylella fastidiosa subsp. multiplex and pauca on olive under controlled conditions |
| title_full |
Pathogenicity and systemic colonization of Spanish strains of Xylella fastidiosa subsp. multiplex and pauca on olive under controlled conditions |
| title_fullStr |
Pathogenicity and systemic colonization of Spanish strains of Xylella fastidiosa subsp. multiplex and pauca on olive under controlled conditions |
| title_full_unstemmed |
Pathogenicity and systemic colonization of Spanish strains of Xylella fastidiosa subsp. multiplex and pauca on olive under controlled conditions |
| title_sort |
pathogenicity and systemic colonization of spanish strains of xylella fastidiosa subsp. multiplex and pauca on olive under controlled conditions |
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2021-04 |
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http://hdl.handle.net/10261/267093 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 |
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AT romanecijamiguel pathogenicityandsystemiccolonizationofspanishstrainsofxylellafastidiosasubspmultiplexandpaucaonoliveundercontrolledconditions AT olivaresgarciaconcepcion pathogenicityandsystemiccolonizationofspanishstrainsofxylellafastidiosasubspmultiplexandpaucaonoliveundercontrolledconditions AT dominguezcalerocristina pathogenicityandsystemiccolonizationofspanishstrainsofxylellafastidiosasubspmultiplexandpaucaonoliveundercontrolledconditions AT navascortesjuanantonio pathogenicityandsystemiccolonizationofspanishstrainsofxylellafastidiosasubspmultiplexandpaucaonoliveundercontrolledconditions AT landablancab pathogenicityandsystemiccolonizationofspanishstrainsofxylellafastidiosasubspmultiplexandpaucaonoliveundercontrolledconditions |
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1777663335074889728 |
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dig-ias-es-10261-2670932022-04-19T01:48:51Z Pathogenicity and systemic colonization of Spanish strains of Xylella fastidiosa subsp. multiplex and pauca on olive under controlled conditions Román Ecija, Miguel Olivares-García, Concepción Domínguez-Calero, Cristina Navas Cortés, Juan Antonio Landa, Blanca B. European Commission Agencia Estatal de Investigación (España) CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Interprofesional del Aceite de Oliva Español Plant health Xylella Trabajo presentado en la 3rd European Conference on Xylella fastidiosa (Building knowledge, protecting plant health), celebrada online el 29 y 30 de abril de 2021. Since the first detection of Xylella fastidiosa (Xf) in the Balearic Islands and Alicante province (mainland Spain), research has provided new insights on genetic diversity and host range of the strains present in the different Spanish outbreaks. The high genetic diversity of Xf in Spain (three subspecies and four STs) and the high host range (over 30) in total implies a risk for several host of agronomic interest if these genotypes spread to areas where they are not present yet. The main goal of this study was to evaluate the pathogenicity and the systemic colonization of four Spanish strains on three olive cultivars representative of the most widely grown in Spain. Two experiments are being conducted in a confined growth chamber (26ºC day/24ºC night), where 10-month-old self-rooted micro-propagated olive cultivars (Arbequina, Hojiblanca and Picual) were inoculated with the strains XYL1961 of Xf subsp. pauca ST80 and the Italian strain De Donno of Xf subsp. pauca ST53 (Control), strain XYL1966 of Xf subsp. multiplex ST81 isolated from olive trees in the Balearic Islands, and the strains IVIA5901 and ESVL of Xf subsp. multiplex ST6 isolated from almond trees in the Guadalest valley (Alicante). Leaf samplings were carried out at the inoculation point (IP), and at 5, 10, 15 and 20 cm above the IP, at 1, 3, 6, 12 and 18 months post-inoculation (mpi), respectively. Furthermore, destructive sampling of different sections of the main stem was performed at 9 mpi at the same levels of leaf sampling. qPCR tests were used to determine the presence and systemic colonization of the bacterium. After 2 years, no clear or specific symptoms associated to Xf infection has been observed. Our results show that all the strains of Xf subsp. multiplex were detected in the leaf petioles at the IP and 5 cm above it although at low frequency (<33%) but were not detected after 6 mpi on samples at 15-cm above the IP. In parallel, the strains were detected in the most upper part of the main stem, although in a very low proportion of samples (8-42% depending on the strain) and with low inoculum densities (Cq > 34) indicating that the strains had some trouble colonizing the different olive genotypes. On the contrary, Xf subsp. pauca strains could be detected on all leaf petiole samples even up to 20 cm over the IP, although the frequency of positive samples and bacterial inoculum decreased drastically from the IP. When sampling the main stem, results indicated that the Spanish strain of Xf subsp. pauca ST80 colonized a higher number of plants and multiplied better than the Italian strain of Xf subsp. pauca ST53 which indicated the risk that this strain may represent for olive. No clear differences could be observed among the patterns of infection of the different Xf strains on the three olive cultivars tested. This work has received funding from 727987 XF-ACTORS (H2020-UE), E-RTA2017-00004-C06-02 (AEI-INIA Spain and FEDER), and the Spanish Olive Oil Interprofessional. 2022-04-18T12:30:03Z 2022-04-18T12:30:03Z 2021-04 2022-04-18T12:30:03Z póster de congreso http://purl.org/coar/resource_type/c_6670 doi: 10.5281/zenodo.4672355 3rd European Conference on Xylella fastidiosa (2021) http://hdl.handle.net/10261/267093 10.5281/zenodo.4672355 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/727987 https://doi.org/10.5281/zenodo.4672355 Sí open |