Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix
Partitiviruses (dsRNA viruses, family Partitiviridae) are ubiquitously detected in plants and fungi. Although previous surveys suggested their omnipresence in the white root rot fungus, Rosellinia necatrix, only a few of them have been molecularly and biologically characterized thus far. We report the characterization of a total of 20 partitiviruses from 16 R. necatrix strains belonging to 15 new species, for which “Rosellinia necatrix partitivirus 11–Rosellinia necatrix partitivirus 25” were proposed, and 5 previously reported species. The newly identified partitiviruses have been taxonomically placed in two genera, Alphapartitivirus, and Betapartitivirus. Some partitiviruses were transfected into reference strains of the natural host, R. necatrix, and an experimental host, Cryphonectria parasitica, using purified virions. A comparative analysis of resultant transfectants revealed interesting differences and similarities between the RNA accumulation and symptom induction patterns of R. necatrix and C. parasitica. Other interesting findings include the identification of a probable reassortment event and a quintuple partitivirus infection of a single fungal strain. These combined results provide a foundation for further studies aimed at elucidating mechanisms that underly the differences observed.
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Frontiers Media
2020-06-26
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Subjects: | Partitivirus, dsRNA virus, Phytopathogenic fungus, Rosellinia necatrix, Cryphonectria parasitica, Diversity, Reassortment, Horizontal transfer, |
Online Access: | http://hdl.handle.net/10261/227233 http://dx.doi.org/10.13039/501100001700 |
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dig-ias-es-10261-2272332021-01-26T04:45:52Z Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix Telengech, Paul Hisano, Sakae Mugambi, Cyrus Hyodo, Kiwamu Arjona-López, J. M. López Herrera, Carlos Kanematsu, Satoko Kondo, Hideki Suzuki, Nobuhiro Yomogi Ministry of Education, Culture, Sports, Science and Technology (Japan) Partitivirus dsRNA virus Phytopathogenic fungus Rosellinia necatrix Cryphonectria parasitica Diversity Reassortment Horizontal transfer Partitiviruses (dsRNA viruses, family Partitiviridae) are ubiquitously detected in plants and fungi. Although previous surveys suggested their omnipresence in the white root rot fungus, Rosellinia necatrix, only a few of them have been molecularly and biologically characterized thus far. We report the characterization of a total of 20 partitiviruses from 16 R. necatrix strains belonging to 15 new species, for which “Rosellinia necatrix partitivirus 11–Rosellinia necatrix partitivirus 25” were proposed, and 5 previously reported species. The newly identified partitiviruses have been taxonomically placed in two genera, Alphapartitivirus, and Betapartitivirus. Some partitiviruses were transfected into reference strains of the natural host, R. necatrix, and an experimental host, Cryphonectria parasitica, using purified virions. A comparative analysis of resultant transfectants revealed interesting differences and similarities between the RNA accumulation and symptom induction patterns of R. necatrix and C. parasitica. Other interesting findings include the identification of a probable reassortment event and a quintuple partitivirus infection of a single fungal strain. These combined results provide a foundation for further studies aimed at elucidating mechanisms that underly the differences observed. This work was supported in part by Yomogi Inc. (to NS), the Program for the Promotion of Basic and Applied Research for Innovation in Bio-Oriented Industries (to SK and NS), and Grants-in-Aid for Scientific Research (A) and on Innovative Areas from the Japanese Ministry of Education, Culture, Sports, Science, and Technology (MEXT) (17H01463 and 16H06436, 16H06429, and 16K21723 to NS). 2021-01-21T12:00:32Z 2021-01-21T12:00:32Z 2020-06-26 2021-01-21T12:00:33Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.3389/fmicb.2020.01064 e-issn: 1664-302X Frontiers in Microbiology 11: 1064 (2020) http://hdl.handle.net/10261/227233 10.3389/fmicb.2020.01064 http://dx.doi.org/10.13039/501100001700 Publisher's version http://doi.org/10.3389/fmicb.2020.01064 Sí open Frontiers Media |
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Partitivirus dsRNA virus Phytopathogenic fungus Rosellinia necatrix Cryphonectria parasitica Diversity Reassortment Horizontal transfer Partitivirus dsRNA virus Phytopathogenic fungus Rosellinia necatrix Cryphonectria parasitica Diversity Reassortment Horizontal transfer |
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Partitivirus dsRNA virus Phytopathogenic fungus Rosellinia necatrix Cryphonectria parasitica Diversity Reassortment Horizontal transfer Partitivirus dsRNA virus Phytopathogenic fungus Rosellinia necatrix Cryphonectria parasitica Diversity Reassortment Horizontal transfer Telengech, Paul Hisano, Sakae Mugambi, Cyrus Hyodo, Kiwamu Arjona-López, J. M. López Herrera, Carlos Kanematsu, Satoko Kondo, Hideki Suzuki, Nobuhiro Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix |
description |
Partitiviruses (dsRNA viruses, family Partitiviridae) are ubiquitously detected in plants and fungi. Although previous surveys suggested their omnipresence in the white root rot fungus, Rosellinia necatrix, only a few of them have been molecularly and biologically characterized thus far. We report the characterization of a total of 20 partitiviruses from 16 R. necatrix strains belonging to 15 new species, for which “Rosellinia necatrix partitivirus 11–Rosellinia necatrix partitivirus 25” were proposed, and 5 previously reported species. The newly identified partitiviruses have been taxonomically placed in two genera, Alphapartitivirus, and Betapartitivirus. Some partitiviruses were transfected into reference strains of the natural host, R. necatrix, and an experimental host, Cryphonectria parasitica, using purified virions. A comparative analysis of resultant transfectants revealed interesting differences and similarities between the RNA accumulation and symptom induction patterns of R. necatrix and C. parasitica. Other interesting findings include the identification of a probable reassortment event and a quintuple partitivirus infection of a single fungal strain. These combined results provide a foundation for further studies aimed at elucidating mechanisms that underly the differences observed. |
author2 |
Yomogi |
author_facet |
Yomogi Telengech, Paul Hisano, Sakae Mugambi, Cyrus Hyodo, Kiwamu Arjona-López, J. M. López Herrera, Carlos Kanematsu, Satoko Kondo, Hideki Suzuki, Nobuhiro |
format |
artículo |
topic_facet |
Partitivirus dsRNA virus Phytopathogenic fungus Rosellinia necatrix Cryphonectria parasitica Diversity Reassortment Horizontal transfer |
author |
Telengech, Paul Hisano, Sakae Mugambi, Cyrus Hyodo, Kiwamu Arjona-López, J. M. López Herrera, Carlos Kanematsu, Satoko Kondo, Hideki Suzuki, Nobuhiro |
author_sort |
Telengech, Paul |
title |
Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix |
title_short |
Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix |
title_full |
Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix |
title_fullStr |
Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix |
title_full_unstemmed |
Diverse Partitiviruses From the Phytopathogenic Fungus, Rosellinia necatrix |
title_sort |
diverse partitiviruses from the phytopathogenic fungus, rosellinia necatrix |
publisher |
Frontiers Media |
publishDate |
2020-06-26 |
url |
http://hdl.handle.net/10261/227233 http://dx.doi.org/10.13039/501100001700 |
work_keys_str_mv |
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