Global Distribution of the Reniform Nematode Genus Rotylenchulus with the Synonymy of Rotylenchulus macrosoma with Rotylenchulus borealis
Reniform nematodes of the genus Rotylenchulus are semi-endoparasites of numerous herbaceous and woody plant roots that occur largely in regions with temperate, subtropical, and tropical climates. In this study, we compared 12 populations of Rotylenchulusborealis and 16 populations of Rotylenchulusmacrosoma, including paratypes deposited in nematode collections, confirming that morphological characters between both nematode species do not support their separation. In addition, analysis of molecular markers using nuclear ribosomal DNA (28S, ITS1) and mitochondrial DNA (coxI) genes, as well as phylogenetic approaches, confirmed the synonymy of R. macrosoma with R. borealis. This study also demonstrated that R. borealis (= macrosoma) from Israel has two distinct rRNA gene types in the genome, specifically the two types of D2-D3 (A and B). We provide a global geographical distribution of the genus Rotylenchulus. The two major pathogenic species (Rotylenchulusreniformis and Rotylenchulusparvus) showed their close relationship with warmer areas with high annual mean temperature, maximum temperature of the warmest month, and minimum temperature of the coldest month. The present study confirms the extraordinary morphological and molecular diversity of R. borealis in Europe, Africa, and the Middle East and comprises a paradigmatic example of remarkable flexibility of ecological requirements within reniform nematodes.
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| Language: | English |
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Multidisciplinary Digital Publishing Institute
2020-12-23
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| Subjects: | Bayesian inference, Cytochrome c oxidase subunit 1, Distribution, D2-D3 expansion domains of 28S rRNA gene, Phylogeny, |
| Online Access: | http://hdl.handle.net/10261/225533 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100005156 http://dx.doi.org/10.13039/501100003339 |
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dig-ias-es-10261-2255332021-12-27T16:19:44Z Global Distribution of the Reniform Nematode Genus Rotylenchulus with the Synonymy of Rotylenchulus macrosoma with Rotylenchulus borealis Palomares Rius, Juan E. Clavero-Camacho, Ilenia Archidona-Yuste, Antonio Cantalapiedra-Navarrete, C. León-Ropero, Guillermo Braun Miyara, Sigal Karssen, Gerrit Castillo, Pablo Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) Consejo Superior de Investigaciones Científicas (España) Alexander von Humboldt Foundation Bayesian inference Cytochrome c oxidase subunit 1 Distribution D2-D3 expansion domains of 28S rRNA gene Phylogeny Reniform nematodes of the genus Rotylenchulus are semi-endoparasites of numerous herbaceous and woody plant roots that occur largely in regions with temperate, subtropical, and tropical climates. In this study, we compared 12 populations of Rotylenchulusborealis and 16 populations of Rotylenchulusmacrosoma, including paratypes deposited in nematode collections, confirming that morphological characters between both nematode species do not support their separation. In addition, analysis of molecular markers using nuclear ribosomal DNA (28S, ITS1) and mitochondrial DNA (coxI) genes, as well as phylogenetic approaches, confirmed the synonymy of R. macrosoma with R. borealis. This study also demonstrated that R. borealis (= macrosoma) from Israel has two distinct rRNA gene types in the genome, specifically the two types of D2-D3 (A and B). We provide a global geographical distribution of the genus Rotylenchulus. The two major pathogenic species (Rotylenchulusreniformis and Rotylenchulusparvus) showed their close relationship with warmer areas with high annual mean temperature, maximum temperature of the warmest month, and minimum temperature of the coldest month. The present study confirms the extraordinary morphological and molecular diversity of R. borealis in Europe, Africa, and the Middle East and comprises a paradigmatic example of remarkable flexibility of ecological requirements within reniform nematodes. This research was supported by grant RTI2018-095925-A-100 from Ministerio de Ciencia, Innovación y Universidades, Spain, grant 201740E042 “Análisis de diversidad molecular, barcoding, y relaciones filogenéticas de nematodos fitoparásitos en cultivos mediterráneos” from the Spanish National Research Council (C.S.I.C.), and by the Humboldt Research Fellowship for Postdoctoral Researchers awarded to the third author (A.A.-Y.). Peer reviewed 2020-12-28T07:48:15Z 2020-12-28T07:48:15Z 2020-12-23 artículo http://purl.org/coar/resource_type/c_6501 Plants 10(1): 7 (2020) http://hdl.handle.net/10261/225533 10.3390/plants10010007 2223-7747 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100005156 http://dx.doi.org/10.13039/501100003339 33374728 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095925-A-100 Publisher's version https://doi.org/10.3390/plants10010007 Sí open Multidisciplinary Digital Publishing Institute |
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Bayesian inference Cytochrome c oxidase subunit 1 Distribution D2-D3 expansion domains of 28S rRNA gene Phylogeny Bayesian inference Cytochrome c oxidase subunit 1 Distribution D2-D3 expansion domains of 28S rRNA gene Phylogeny |
| spellingShingle |
Bayesian inference Cytochrome c oxidase subunit 1 Distribution D2-D3 expansion domains of 28S rRNA gene Phylogeny Bayesian inference Cytochrome c oxidase subunit 1 Distribution D2-D3 expansion domains of 28S rRNA gene Phylogeny Palomares Rius, Juan E. Clavero-Camacho, Ilenia Archidona-Yuste, Antonio Cantalapiedra-Navarrete, C. León-Ropero, Guillermo Braun Miyara, Sigal Karssen, Gerrit Castillo, Pablo Global Distribution of the Reniform Nematode Genus Rotylenchulus with the Synonymy of Rotylenchulus macrosoma with Rotylenchulus borealis |
| description |
Reniform nematodes of the genus Rotylenchulus are semi-endoparasites of numerous herbaceous and woody plant roots that occur largely in regions with temperate, subtropical, and tropical climates. In this study, we compared 12 populations of Rotylenchulusborealis and 16 populations of Rotylenchulusmacrosoma, including paratypes deposited in nematode collections, confirming that morphological characters between both nematode species do not support their separation. In addition, analysis of molecular markers using nuclear ribosomal DNA (28S, ITS1) and mitochondrial DNA (coxI) genes, as well as phylogenetic approaches, confirmed the synonymy of R. macrosoma with R. borealis. This study also demonstrated that R. borealis (= macrosoma) from Israel has two distinct rRNA gene types in the genome, specifically the two types of D2-D3 (A and B). We provide a global geographical distribution of the genus Rotylenchulus. The two major pathogenic species (Rotylenchulusreniformis and Rotylenchulusparvus) showed their close relationship with warmer areas with high annual mean temperature, maximum temperature of the warmest month, and minimum temperature of the coldest month. The present study confirms the extraordinary morphological and molecular diversity of R. borealis in Europe, Africa, and the Middle East and comprises a paradigmatic example of remarkable flexibility of ecological requirements within reniform nematodes. |
| author2 |
Ministerio de Ciencia, Innovación y Universidades (España) |
| author_facet |
Ministerio de Ciencia, Innovación y Universidades (España) Palomares Rius, Juan E. Clavero-Camacho, Ilenia Archidona-Yuste, Antonio Cantalapiedra-Navarrete, C. León-Ropero, Guillermo Braun Miyara, Sigal Karssen, Gerrit Castillo, Pablo |
| format |
artículo |
| topic_facet |
Bayesian inference Cytochrome c oxidase subunit 1 Distribution D2-D3 expansion domains of 28S rRNA gene Phylogeny |
| author |
Palomares Rius, Juan E. Clavero-Camacho, Ilenia Archidona-Yuste, Antonio Cantalapiedra-Navarrete, C. León-Ropero, Guillermo Braun Miyara, Sigal Karssen, Gerrit Castillo, Pablo |
| author_sort |
Palomares Rius, Juan E. |
| title |
Global Distribution of the Reniform Nematode Genus Rotylenchulus with the Synonymy of Rotylenchulus macrosoma with Rotylenchulus borealis |
| title_short |
Global Distribution of the Reniform Nematode Genus Rotylenchulus with the Synonymy of Rotylenchulus macrosoma with Rotylenchulus borealis |
| title_full |
Global Distribution of the Reniform Nematode Genus Rotylenchulus with the Synonymy of Rotylenchulus macrosoma with Rotylenchulus borealis |
| title_fullStr |
Global Distribution of the Reniform Nematode Genus Rotylenchulus with the Synonymy of Rotylenchulus macrosoma with Rotylenchulus borealis |
| title_full_unstemmed |
Global Distribution of the Reniform Nematode Genus Rotylenchulus with the Synonymy of Rotylenchulus macrosoma with Rotylenchulus borealis |
| title_sort |
global distribution of the reniform nematode genus rotylenchulus with the synonymy of rotylenchulus macrosoma with rotylenchulus borealis |
| publisher |
Multidisciplinary Digital Publishing Institute |
| publishDate |
2020-12-23 |
| url |
http://hdl.handle.net/10261/225533 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/100005156 http://dx.doi.org/10.13039/501100003339 |
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