Temperature determines growth and biofilm formation of Xylella fastidiosastrains in vitro

Temperature determines growth and biofilm formation of Xylella fastidiosastrains in vitro

Trabajo presentado en la 2nd European conference on Xylella fastidiosa (how research can support solutions), celebrada en Ajaccio el 29 y 30 de octubre de 2019.

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Main Authors: Román Ecija, Miguel, Landa, Blanca B., Olivares-García, Concepción, Jiménez Díaz, Rafael Manuel, Navas Cortés, Juan Antonio
Other Authors: European Commission
Format: comunicación de congreso biblioteca
Language:English
Published: 2019-10
Online Access:http://hdl.handle.net/10261/208265
http://dx.doi.org/10.13039/100007652
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100011033
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spelling dig-ias-es-10261-2082652020-04-21T06:29:55Z Temperature determines growth and biofilm formation of Xylella fastidiosastrains in vitro Román Ecija, Miguel Landa, Blanca B. Olivares-García, Concepción Jiménez Díaz, Rafael Manuel Navas Cortés, Juan Antonio European Commission Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Interprofesional del Aceite de Oliva Español Trabajo presentado en la 2nd European conference on Xylella fastidiosa (how research can support solutions), celebrada en Ajaccio el 29 y 30 de octubre de 2019. Xylella fastidiosa (Xf) is classified as a mesophilic bacterium particularly well adapted to areas with mild winters. The effects of temperature on Xf development have been addressed only for Xf subsp. fastidiosa strains. In this work, we have explored the effects of temperature on growth and biofilm formation in vitro for Xf strains representative of Xf subspecies prevalent in the European outbreaks. Growth, biofilm formation and cell viability of Xf strains from subsp. fastidiosa (7 strains), multiplex (18 strains), pauca (3 strains) and sandyi (2 strains) of different geographic and host origin have been determined in microplates with PD3 medium incubated at a constant temperature range of 6–32 °C at 4 °C intervals. Growth was determined daily by optical density at 600 nm, and biofilm was assessed at the end of the experiments by a crystal violet assay. Both parameters were modelled using a beta function to estimate the cardinal and optimal temperature values for each strain. Our results indicate that both growth and biofilm formation is determined by temperature and Xf strain. Overall, minimum vales for both parameters were observed at the extreme temperatures of 6 and 32 °C. However, the range with optimal values largely depends on the Xf strain. Although no distinct range was associated with subspecies, the widest temperature range for optimal growth was estimated for Xf subsp. multiplex strains at 20–32 °C. Optimal growth was estimated at 24–32 °C for Xf subsp. fastidiosa strains. No clear pattern was found for Xf subsp. pauca, with optimal values between 16 and 24 °C or 20 and 28 °C, depending on the strains. Similarly, for Xf subsp. sandyi, optimal growth occurred at 16–28 °C or 20–28 °C. With a few exceptions, similar response to temperature was observed for biofilm formation.These results would allow the development of region-specific epidemiological models to predict the risk associated with the establishment and spread of Xf strains in Europe. This work has received funding from 727987 XF-ACTORS (EU-H2020), E-RTA2017-00004-C06-02 from AEI-INIA Spain and FEDER, and the Spanish Olive Oil Interprofessional. Peer reviewed 2020-04-20T09:46:33Z 2020-04-20T09:46:33Z 2019-10 comunicación de congreso http://purl.org/coar/resource_type/c_5794 2nd European conference on Xylella fastidiosa (2019) http://hdl.handle.net/10261/208265 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011033 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/727987 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/E-RTA2017-00004-C06-02 E-RTA2017-00004-C06-02/AEI/10.13039/501100011033 Sí open
institution IAS ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ias-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IAS España
language English
description Trabajo presentado en la 2nd European conference on Xylella fastidiosa (how research can support solutions), celebrada en Ajaccio el 29 y 30 de octubre de 2019.
author2 European Commission
author_facet European Commission
Román Ecija, Miguel
Landa, Blanca B.
Olivares-García, Concepción
Jiménez Díaz, Rafael Manuel
Navas Cortés, Juan Antonio
format comunicación de congreso
author Román Ecija, Miguel
Landa, Blanca B.
Olivares-García, Concepción
Jiménez Díaz, Rafael Manuel
Navas Cortés, Juan Antonio
spellingShingle Román Ecija, Miguel
Landa, Blanca B.
Olivares-García, Concepción
Jiménez Díaz, Rafael Manuel
Navas Cortés, Juan Antonio
Temperature determines growth and biofilm formation of Xylella fastidiosastrains in vitro
author_sort Román Ecija, Miguel
title Temperature determines growth and biofilm formation of Xylella fastidiosastrains in vitro
title_short Temperature determines growth and biofilm formation of Xylella fastidiosastrains in vitro
title_full Temperature determines growth and biofilm formation of Xylella fastidiosastrains in vitro
title_fullStr Temperature determines growth and biofilm formation of Xylella fastidiosastrains in vitro
title_full_unstemmed Temperature determines growth and biofilm formation of Xylella fastidiosastrains in vitro
title_sort temperature determines growth and biofilm formation of xylella fastidiosastrains in vitro
publishDate 2019-10
url http://hdl.handle.net/10261/208265
http://dx.doi.org/10.13039/100007652
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100011033
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AT jimenezdiazrafaelmanuel temperaturedeterminesgrowthandbiofilmformationofxylellafastidiosastrainsinvitro
AT navascortesjuanantonio temperaturedeterminesgrowthandbiofilmformationofxylellafastidiosastrainsinvitro
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