Transcriptomics, chromosome engineering and mapping identify a restorer-of-fertility region in the CMS wheat system msH1
Cytoplasmic male sterility (CMS) is a valuable trait for hybrid seed production. The msH1 CMS system in common wheat results from the incompatibility between the nuclear genome of wheat and the cytoplasm of the wild barley Hordeum chilense. This work aims to identify H. chilense candidate genes for fertility restoration in the msH1 system with a multidisciplinary strategy based on chromosome engineering, differential expression analysis and genome mapping. Alloplasmic isogenic wheat lines differing for fertility, associated with the presence of an acrocentric chromosome Hchac resulting from the rearrangement of the short arms of H. chilense chromosomes 1Hch and 6Hch, were used for transcriptome sequencing. Two novel RNA-seq mapping approaches were designed and compared to identify differentially expressed genes of H. chilense associated with male fertility restoration. Minichromosomes (Hchmi), new smaller reorganizations of the Hchac also restoring fertility, were obtained and used to validate the candidate genes. This strategy was successful identifying a putative restorer-of-fertility region on 6HchS, with six candidate genes, including the ortholog of the barley restorer gene Rfm1. Additionally, transcriptomics gave preliminary insights on sterility and restoration networks showing the importance of energy supply, stress, protein metabolism and RNA processing.
Main Authors: | , , , , , , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Springer Nature
2019-10-17
|
Subjects: | Cytoplasmic male sterility, Common wheat, Hordeum chilense, Minichromosomes, Restorer-of-fertility, RNA-seq, |
Online Access: | http://hdl.handle.net/10261/205472 http://dx.doi.org/10.13039/501100003329 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-ias-es-10261-205472 |
---|---|
record_format |
koha |
spelling |
dig-ias-es-10261-2054722020-10-17T04:30:37Z Transcriptomics, chromosome engineering and mapping identify a restorer-of-fertility region in the CMS wheat system msH1 Rodríguez-Suárez, Cristina Bagnaresi, Paolo Cattivelli, Luigi Pistón, Fernando Castillo, Almudena Martín Ramírez, Azahara Carmen Atienza, Sergio G. Ramírez, M. Carmen Martín, Antonio Ministerio de Economía y Competitividad (España) Cytoplasmic male sterility Common wheat Hordeum chilense Minichromosomes Restorer-of-fertility RNA-seq Cytoplasmic male sterility (CMS) is a valuable trait for hybrid seed production. The msH1 CMS system in common wheat results from the incompatibility between the nuclear genome of wheat and the cytoplasm of the wild barley Hordeum chilense. This work aims to identify H. chilense candidate genes for fertility restoration in the msH1 system with a multidisciplinary strategy based on chromosome engineering, differential expression analysis and genome mapping. Alloplasmic isogenic wheat lines differing for fertility, associated with the presence of an acrocentric chromosome Hchac resulting from the rearrangement of the short arms of H. chilense chromosomes 1Hch and 6Hch, were used for transcriptome sequencing. Two novel RNA-seq mapping approaches were designed and compared to identify differentially expressed genes of H. chilense associated with male fertility restoration. Minichromosomes (Hchmi), new smaller reorganizations of the Hchac also restoring fertility, were obtained and used to validate the candidate genes. This strategy was successful identifying a putative restorer-of-fertility region on 6HchS, with six candidate genes, including the ortholog of the barley restorer gene Rfm1. Additionally, transcriptomics gave preliminary insights on sterility and restoration networks showing the importance of energy supply, stress, protein metabolism and RNA processing. This research was funded by Grants AGL2013-43329-R and SOLAUT_00025062, from Ministerio de Economía y Competitividad, Spain (MINECO). Peer reviewed 2020-03-27T10:40:00Z 2020-03-27T10:40:00Z 2019-10-17 artículo http://purl.org/coar/resource_type/c_6501 TAG Theoretical and Applied Genetics 133: 283-295 (2020) 0040-5752 http://hdl.handle.net/10261/205472 10.1007/s00122-019-03457-3 1432-2242 http://dx.doi.org/10.13039/501100003329 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-43329-R Postprint http://dx.doi.org/10.1007/s00122-019-03457-3 Sí open Springer Nature |
institution |
IAS ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-ias-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del IAS España |
language |
English |
topic |
Cytoplasmic male sterility Common wheat Hordeum chilense Minichromosomes Restorer-of-fertility RNA-seq Cytoplasmic male sterility Common wheat Hordeum chilense Minichromosomes Restorer-of-fertility RNA-seq |
spellingShingle |
Cytoplasmic male sterility Common wheat Hordeum chilense Minichromosomes Restorer-of-fertility RNA-seq Cytoplasmic male sterility Common wheat Hordeum chilense Minichromosomes Restorer-of-fertility RNA-seq Rodríguez-Suárez, Cristina Bagnaresi, Paolo Cattivelli, Luigi Pistón, Fernando Castillo, Almudena Martín Ramírez, Azahara Carmen Atienza, Sergio G. Ramírez, M. Carmen Martín, Antonio Transcriptomics, chromosome engineering and mapping identify a restorer-of-fertility region in the CMS wheat system msH1 |
description |
Cytoplasmic male sterility (CMS) is a valuable trait for hybrid seed production. The msH1 CMS system in common wheat results from the incompatibility between the nuclear genome of wheat and the cytoplasm of the wild barley Hordeum chilense. This work aims to identify H. chilense candidate genes for fertility restoration in the msH1 system with a multidisciplinary strategy based on chromosome engineering, differential expression analysis and genome mapping. Alloplasmic isogenic wheat lines differing for fertility, associated with the presence of an acrocentric chromosome Hchac resulting from the rearrangement of the short arms of H. chilense chromosomes 1Hch and 6Hch, were used for transcriptome sequencing. Two novel RNA-seq mapping approaches were designed and compared to identify differentially expressed genes of H. chilense associated with male fertility restoration. Minichromosomes (Hchmi), new smaller reorganizations of the Hchac also restoring fertility, were obtained and used to validate the candidate genes. This strategy was successful identifying a putative restorer-of-fertility region on 6HchS, with six candidate genes, including the ortholog of the barley restorer gene Rfm1. Additionally, transcriptomics gave preliminary insights on sterility and restoration networks showing the importance of energy supply, stress, protein metabolism and RNA processing. |
author2 |
Ministerio de Economía y Competitividad (España) |
author_facet |
Ministerio de Economía y Competitividad (España) Rodríguez-Suárez, Cristina Bagnaresi, Paolo Cattivelli, Luigi Pistón, Fernando Castillo, Almudena Martín Ramírez, Azahara Carmen Atienza, Sergio G. Ramírez, M. Carmen Martín, Antonio |
format |
artículo |
topic_facet |
Cytoplasmic male sterility Common wheat Hordeum chilense Minichromosomes Restorer-of-fertility RNA-seq |
author |
Rodríguez-Suárez, Cristina Bagnaresi, Paolo Cattivelli, Luigi Pistón, Fernando Castillo, Almudena Martín Ramírez, Azahara Carmen Atienza, Sergio G. Ramírez, M. Carmen Martín, Antonio |
author_sort |
Rodríguez-Suárez, Cristina |
title |
Transcriptomics, chromosome engineering and mapping identify a restorer-of-fertility region in the CMS wheat system msH1 |
title_short |
Transcriptomics, chromosome engineering and mapping identify a restorer-of-fertility region in the CMS wheat system msH1 |
title_full |
Transcriptomics, chromosome engineering and mapping identify a restorer-of-fertility region in the CMS wheat system msH1 |
title_fullStr |
Transcriptomics, chromosome engineering and mapping identify a restorer-of-fertility region in the CMS wheat system msH1 |
title_full_unstemmed |
Transcriptomics, chromosome engineering and mapping identify a restorer-of-fertility region in the CMS wheat system msH1 |
title_sort |
transcriptomics, chromosome engineering and mapping identify a restorer-of-fertility region in the cms wheat system msh1 |
publisher |
Springer Nature |
publishDate |
2019-10-17 |
url |
http://hdl.handle.net/10261/205472 http://dx.doi.org/10.13039/501100003329 |
work_keys_str_mv |
AT rodriguezsuarezcristina transcriptomicschromosomeengineeringandmappingidentifyarestoreroffertilityregioninthecmswheatsystemmsh1 AT bagnaresipaolo transcriptomicschromosomeengineeringandmappingidentifyarestoreroffertilityregioninthecmswheatsystemmsh1 AT cattivelliluigi transcriptomicschromosomeengineeringandmappingidentifyarestoreroffertilityregioninthecmswheatsystemmsh1 AT pistonfernando transcriptomicschromosomeengineeringandmappingidentifyarestoreroffertilityregioninthecmswheatsystemmsh1 AT castilloalmudena transcriptomicschromosomeengineeringandmappingidentifyarestoreroffertilityregioninthecmswheatsystemmsh1 AT martinramirezazaharacarmen transcriptomicschromosomeengineeringandmappingidentifyarestoreroffertilityregioninthecmswheatsystemmsh1 AT atienzasergiog transcriptomicschromosomeengineeringandmappingidentifyarestoreroffertilityregioninthecmswheatsystemmsh1 AT ramirezmcarmen transcriptomicschromosomeengineeringandmappingidentifyarestoreroffertilityregioninthecmswheatsystemmsh1 AT martinantonio transcriptomicschromosomeengineeringandmappingidentifyarestoreroffertilityregioninthecmswheatsystemmsh1 |
_version_ |
1777663249857118208 |