The Strawberry FaWRKY1 Transcription Factor Negatively Regulates Resistance to Colletotrichum acutatum in Fruit Upon Infection
Strawberry (Fragaria ×ananassa) is a major food crop worldwide, due to the flavor, aroma and health benefits of the fruit, but its productivity and quality are seriously limited by a large variety of phytopathogens, including Colletotrichum spp. So far, key factors regulating strawberry immune response remain unknown. The FaWRKY1 gene has been previously proposed as an important element mediating defense responses in strawberry to Colletotrichum acutatum. To get further insight into the functional role that FaWRKY1 plays in the defense mechanism, Agrobacterium-mediated transient transformation was used both to silence and overexpress the FaWRKY1 gene in strawberry fruits (Fragaria ×ananassa cv. Primoris), which were later analyzed upon C. acutatum inoculation. Susceptibility tests were performed after pathogen infection comparing the severity of disease between the two agroinfiltrated opposite halves of the same fruit, one half bearing a construct either for FaWRKY1 overexpression or RNAi-mediated silencing and the other half bearing the empty vector, as control. The severity of tissue damage was monitored and found to be visibly reduced at five days after pathogen inoculation in the fruit half where FaWRKY1 was transiently silenced compared to that of the opposite control half and statistical analysis corroborated a significant reduction in disease susceptibility. Contrarily, a similar level of susceptibility was found when FaWRKY1 overexpression and control fruit samples, was compared. These results unravel a negative regulatory role of FaWRKY1 in resistance to the phytopathogenic fungus C. acutatum in strawberry fruit and contrast with the previous role described for this gene in Arabidopsis as positive regulator of resistance against the bacteria Pseudomonas syringae. Based on previous results, a tentative working model for WRKY75 like genes after pathogen infection is proposed and the expression pattern of potential downstream FaWRKY1 target genes was also analyzed in strawberry fruit upon C. acutatum infection. Our results highlight that FaWRKY1 might display different function according to species, plant tissue and/or type of pathogen and underline the intricate FaWRKY1 responsive defense regulatory mechanism taking place in strawberry against this important crop pathogen.
Main Authors: | , , , , , , , , , |
---|---|
Other Authors: | |
Format: | artículo biblioteca |
Language: | English |
Published: |
Frontiers Media
2019-04-18
|
Subjects: | WRKY transcription factor, Colletotrichum acutatum, Agrobacterium transformation, Strawberry fruit defense response, Fragaria ananassa, |
Online Access: | http://hdl.handle.net/10261/205371 http://dx.doi.org/10.13039/501100011011 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-ias-es-10261-205371 |
---|---|
record_format |
koha |
spelling |
dig-ias-es-10261-2053712021-12-28T16:30:52Z The Strawberry FaWRKY1 Transcription Factor Negatively Regulates Resistance to Colletotrichum acutatum in Fruit Upon Infection Higuera-Sobrino, José Javier Garrido Gala, José Lekhbou, Ayman Arjona Girona, Isabel Amil-Ruiz, Francisco Mercado Carmona, J. A. Pliego-Alfaro, Fernando Muñoz-Blanco, Juan López Herrera, Carlos Caballero Repullo, J. L. Junta de Andalucía WRKY transcription factor Colletotrichum acutatum Agrobacterium transformation Strawberry fruit defense response Fragaria ananassa Strawberry (Fragaria ×ananassa) is a major food crop worldwide, due to the flavor, aroma and health benefits of the fruit, but its productivity and quality are seriously limited by a large variety of phytopathogens, including Colletotrichum spp. So far, key factors regulating strawberry immune response remain unknown. The FaWRKY1 gene has been previously proposed as an important element mediating defense responses in strawberry to Colletotrichum acutatum. To get further insight into the functional role that FaWRKY1 plays in the defense mechanism, Agrobacterium-mediated transient transformation was used both to silence and overexpress the FaWRKY1 gene in strawberry fruits (Fragaria ×ananassa cv. Primoris), which were later analyzed upon C. acutatum inoculation. Susceptibility tests were performed after pathogen infection comparing the severity of disease between the two agroinfiltrated opposite halves of the same fruit, one half bearing a construct either for FaWRKY1 overexpression or RNAi-mediated silencing and the other half bearing the empty vector, as control. The severity of tissue damage was monitored and found to be visibly reduced at five days after pathogen inoculation in the fruit half where FaWRKY1 was transiently silenced compared to that of the opposite control half and statistical analysis corroborated a significant reduction in disease susceptibility. Contrarily, a similar level of susceptibility was found when FaWRKY1 overexpression and control fruit samples, was compared. These results unravel a negative regulatory role of FaWRKY1 in resistance to the phytopathogenic fungus C. acutatum in strawberry fruit and contrast with the previous role described for this gene in Arabidopsis as positive regulator of resistance against the bacteria Pseudomonas syringae. Based on previous results, a tentative working model for WRKY75 like genes after pathogen infection is proposed and the expression pattern of potential downstream FaWRKY1 target genes was also analyzed in strawberry fruit upon C. acutatum infection. Our results highlight that FaWRKY1 might display different function according to species, plant tissue and/or type of pathogen and underline the intricate FaWRKY1 responsive defense regulatory mechanism taking place in strawberry against this important crop pathogen. This work was supported by Junta de Andalucía, Spain (Proyectos de Excelencia P12-AGR2174, and grants to Grupo-BIO278). Peer reviewed 2020-03-26T16:53:23Z 2020-03-26T16:53:23Z 2019-04-18 artículo http://purl.org/coar/resource_type/c_6501 Frontiers in Plant Science 10: 480 (2019) http://hdl.handle.net/10261/205371 10.3389/fpls.2019.00480 1664-462X http://dx.doi.org/10.13039/501100011011 31057583 en Publisher's version http://dx.doi.org/10.3389/fpls.2019.00480 Sí open Frontiers Media |
institution |
IAS ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-ias-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del IAS España |
language |
English |
topic |
WRKY transcription factor Colletotrichum acutatum Agrobacterium transformation Strawberry fruit defense response Fragaria ananassa WRKY transcription factor Colletotrichum acutatum Agrobacterium transformation Strawberry fruit defense response Fragaria ananassa |
spellingShingle |
WRKY transcription factor Colletotrichum acutatum Agrobacterium transformation Strawberry fruit defense response Fragaria ananassa WRKY transcription factor Colletotrichum acutatum Agrobacterium transformation Strawberry fruit defense response Fragaria ananassa Higuera-Sobrino, José Javier Garrido Gala, José Lekhbou, Ayman Arjona Girona, Isabel Amil-Ruiz, Francisco Mercado Carmona, J. A. Pliego-Alfaro, Fernando Muñoz-Blanco, Juan López Herrera, Carlos Caballero Repullo, J. L. The Strawberry FaWRKY1 Transcription Factor Negatively Regulates Resistance to Colletotrichum acutatum in Fruit Upon Infection |
description |
Strawberry (Fragaria ×ananassa) is a major food crop worldwide, due to the flavor, aroma and health benefits of the fruit, but its productivity and quality are seriously limited by a large variety of phytopathogens, including Colletotrichum spp. So far, key factors regulating strawberry immune response remain unknown. The FaWRKY1 gene has been previously proposed as an important element mediating defense responses in strawberry to Colletotrichum acutatum. To get further insight into the functional role that FaWRKY1 plays in the defense mechanism, Agrobacterium-mediated transient transformation was used both to silence and overexpress the FaWRKY1 gene in strawberry fruits (Fragaria ×ananassa cv. Primoris), which were later analyzed upon C. acutatum inoculation. Susceptibility tests were performed after pathogen infection comparing the severity of disease between the two agroinfiltrated opposite halves of the same fruit, one half bearing a construct either for FaWRKY1 overexpression or RNAi-mediated silencing and the other half bearing the empty vector, as control. The severity of tissue damage was monitored and found to be visibly reduced at five days after pathogen inoculation in the fruit half where FaWRKY1 was transiently silenced compared to that of the opposite control half and statistical analysis corroborated a significant reduction in disease susceptibility. Contrarily, a similar level of susceptibility was found when FaWRKY1 overexpression and control fruit samples, was compared. These results unravel a negative regulatory role of FaWRKY1 in resistance to the phytopathogenic fungus C. acutatum in strawberry fruit and contrast with the previous role described for this gene in Arabidopsis as positive regulator of resistance against the bacteria Pseudomonas syringae. Based on previous results, a tentative working model for WRKY75 like genes after pathogen infection is proposed and the expression pattern of potential downstream FaWRKY1 target genes was also analyzed in strawberry fruit upon C. acutatum infection. Our results highlight that FaWRKY1 might display different function according to species, plant tissue and/or type of pathogen and underline the intricate FaWRKY1 responsive defense regulatory mechanism taking place in strawberry against this important crop pathogen. |
author2 |
Junta de Andalucía |
author_facet |
Junta de Andalucía Higuera-Sobrino, José Javier Garrido Gala, José Lekhbou, Ayman Arjona Girona, Isabel Amil-Ruiz, Francisco Mercado Carmona, J. A. Pliego-Alfaro, Fernando Muñoz-Blanco, Juan López Herrera, Carlos Caballero Repullo, J. L. |
format |
artículo |
topic_facet |
WRKY transcription factor Colletotrichum acutatum Agrobacterium transformation Strawberry fruit defense response Fragaria ananassa |
author |
Higuera-Sobrino, José Javier Garrido Gala, José Lekhbou, Ayman Arjona Girona, Isabel Amil-Ruiz, Francisco Mercado Carmona, J. A. Pliego-Alfaro, Fernando Muñoz-Blanco, Juan López Herrera, Carlos Caballero Repullo, J. L. |
author_sort |
Higuera-Sobrino, José Javier |
title |
The Strawberry FaWRKY1 Transcription Factor Negatively Regulates Resistance to Colletotrichum acutatum in Fruit Upon Infection |
title_short |
The Strawberry FaWRKY1 Transcription Factor Negatively Regulates Resistance to Colletotrichum acutatum in Fruit Upon Infection |
title_full |
The Strawberry FaWRKY1 Transcription Factor Negatively Regulates Resistance to Colletotrichum acutatum in Fruit Upon Infection |
title_fullStr |
The Strawberry FaWRKY1 Transcription Factor Negatively Regulates Resistance to Colletotrichum acutatum in Fruit Upon Infection |
title_full_unstemmed |
The Strawberry FaWRKY1 Transcription Factor Negatively Regulates Resistance to Colletotrichum acutatum in Fruit Upon Infection |
title_sort |
strawberry fawrky1 transcription factor negatively regulates resistance to colletotrichum acutatum in fruit upon infection |
publisher |
Frontiers Media |
publishDate |
2019-04-18 |
url |
http://hdl.handle.net/10261/205371 http://dx.doi.org/10.13039/501100011011 |
work_keys_str_mv |
AT higuerasobrinojosejavier thestrawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT garridogalajose thestrawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT lekhbouayman thestrawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT arjonagironaisabel thestrawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT amilruizfrancisco thestrawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT mercadocarmonaja thestrawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT pliegoalfarofernando thestrawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT munozblancojuan thestrawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT lopezherreracarlos thestrawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT caballerorepullojl thestrawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT higuerasobrinojosejavier strawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT garridogalajose strawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT lekhbouayman strawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT arjonagironaisabel strawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT amilruizfrancisco strawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT mercadocarmonaja strawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT pliegoalfarofernando strawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT munozblancojuan strawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT lopezherreracarlos strawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection AT caballerorepullojl strawberryfawrky1transcriptionfactornegativelyregulatesresistancetocolletotrichumacutatuminfruituponinfection |
_version_ |
1777663249292984320 |