Suppression of Verticillium wilt in olive planting stocks by root-associated fluorescent Pseudomonas spp

Suppression of Verticillium wilt in olive planting stocks by root-associated fluorescent Pseudomonas spp

13 pages, 5 tables, 1 figure.

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Main Authors: Mercado-Blanco, Jesús, Rodríguez-Jurado, Dolores, Hervás, Ana B., Jiménez-Díaz, Rafael M.
Format: artículo biblioteca
Language:English
Published: Elsevier 2004-06
Subjects:Olea europaea, Pseudomonas fluorescens, Pseudomonas putida, Verticillium dahliae, Biological control, Rhizobacteria, Hydrogen cyanide, Pseudobactin, Salicylic acid, Siderophores,
Online Access:http://hdl.handle.net/10261/20514
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spelling dig-ias-es-10261-205142018-08-03T07:23:48Z Suppression of Verticillium wilt in olive planting stocks by root-associated fluorescent Pseudomonas spp Mercado-Blanco, Jesús Rodríguez-Jurado, Dolores Hervás, Ana B. Jiménez-Díaz, Rafael M. Olea europaea Pseudomonas fluorescens Pseudomonas putida Verticillium dahliae Biological control Rhizobacteria Hydrogen cyanide Pseudobactin Salicylic acid Siderophores 13 pages, 5 tables, 1 figure. Protection of pathogen-free olive planting material from infection by Verticillium dahliae during plant propagation and/or at planting would help in the management of Verticillium wilt of olive. In this study, 8 isolates of Pseudomonas fluorescens (6) and Pseudomonas putida (2) obtained from roots of olive plants were tested for suppression of Verticillium wilt in nursery-produced olive planting stocks under controlled conditions. All tested bacteria produced the green fluorescent siderophore pseudobactin in vitro but only some P. fluorescens isolates produced either salicylic acid in succinate medium or HCN in vitro assays. The antagonistic activity of P. fluorescens and P. putida isolates from olive against defoliating (D) and nondefoliating (ND) V. dahliae pathotypes varied with culture media. On PDA, isolates of P. putida were more inhibitory to the pathogen than those of P. fluorescens. In planta bioassays were conducted either under growth chamber or greenhouse conditions, by inoculating bacterial-treated and -nontreated 3- to 4-month-old, own-rooted or micropropagated plants of susceptible olive cv. Picual with the highly virulent D V. dahliae. Results from three experiments indicated that root treatment with some of P. fluorescens isolates significantly delayed the onset of symptoms, and reduced the final disease incidence and severity by 31–82% and 73–96%, respectively, compared with the nontreated controls, under conditions of severe Verticillium wilt. In addition, those bacteria counteracted the deleterious effects caused by the pathogen infection through enhancement of plant growth. Our results indicate that root treatment of olive plants with selected P. fluorescens isolates during nursery propagation can help in the biocontrol of D V. dahliae in olive. No correlation was found between efficacy of tested bacterial isolates for in vitro antagonism of the pathogen and in planta suppression of Verticillium wilt. Research was supported by Grant CAO-0017 from Junta de Andaluc ıa, Spain. We thank B.B. Landa, Instituto de Agricultura Sostenible, CSIC, for bacterial species determination. Peer reviewed 2010-02-01T10:54:35Z 2010-02-01T10:54:35Z 2004-06 artículo http://purl.org/coar/resource_type/c_6501 Biological Control 30(2): 474-486 (2004) 1049-9644 http://hdl.handle.net/10261/20514 10.1016/j.biocontrol.2004.02.002 en http://dx.doi.org/10.1016/j.biocontrol.2004.02.002 none 259768 bytes application/pdf Elsevier
institution IAS ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-ias-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IAS España
language English
topic Olea europaea
Pseudomonas fluorescens
Pseudomonas putida
Verticillium dahliae
Biological control
Rhizobacteria
Hydrogen cyanide
Pseudobactin
Salicylic acid
Siderophores
Olea europaea
Pseudomonas fluorescens
Pseudomonas putida
Verticillium dahliae
Biological control
Rhizobacteria
Hydrogen cyanide
Pseudobactin
Salicylic acid
Siderophores
spellingShingle Olea europaea
Pseudomonas fluorescens
Pseudomonas putida
Verticillium dahliae
Biological control
Rhizobacteria
Hydrogen cyanide
Pseudobactin
Salicylic acid
Siderophores
Olea europaea
Pseudomonas fluorescens
Pseudomonas putida
Verticillium dahliae
Biological control
Rhizobacteria
Hydrogen cyanide
Pseudobactin
Salicylic acid
Siderophores
Mercado-Blanco, Jesús
Rodríguez-Jurado, Dolores
Hervás, Ana B.
Jiménez-Díaz, Rafael M.
Suppression of Verticillium wilt in olive planting stocks by root-associated fluorescent Pseudomonas spp
description 13 pages, 5 tables, 1 figure.
format artículo
topic_facet Olea europaea
Pseudomonas fluorescens
Pseudomonas putida
Verticillium dahliae
Biological control
Rhizobacteria
Hydrogen cyanide
Pseudobactin
Salicylic acid
Siderophores
author Mercado-Blanco, Jesús
Rodríguez-Jurado, Dolores
Hervás, Ana B.
Jiménez-Díaz, Rafael M.
author_facet Mercado-Blanco, Jesús
Rodríguez-Jurado, Dolores
Hervás, Ana B.
Jiménez-Díaz, Rafael M.
author_sort Mercado-Blanco, Jesús
title Suppression of Verticillium wilt in olive planting stocks by root-associated fluorescent Pseudomonas spp
title_short Suppression of Verticillium wilt in olive planting stocks by root-associated fluorescent Pseudomonas spp
title_full Suppression of Verticillium wilt in olive planting stocks by root-associated fluorescent Pseudomonas spp
title_fullStr Suppression of Verticillium wilt in olive planting stocks by root-associated fluorescent Pseudomonas spp
title_full_unstemmed Suppression of Verticillium wilt in olive planting stocks by root-associated fluorescent Pseudomonas spp
title_sort suppression of verticillium wilt in olive planting stocks by root-associated fluorescent pseudomonas spp
publisher Elsevier
publishDate 2004-06
url http://hdl.handle.net/10261/20514
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AT hervasanab suppressionofverticilliumwiltinoliveplantingstocksbyrootassociatedfluorescentpseudomonasspp
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