Development of SCAR markers in olive (Olea europaea) by direct sequencing of RAPD products: applications in olive germplasm evaluation and mapping
RAPD markers generated by mixtures of two different 10-mer primers were developed for eight different olive cultivars used as parental lines in olive-breeding programs. Two RAPD bands were converted into dominant SCAR markers by direct sequencing of the RAPD products, avoiding the costly and time-consuming cloning step. The SCARs generated have maintained the original RAPD polymorphism among the cultivars and segregated according to Mendelian inheritance. Preliminary results suggest the use of the SCAR SCOeMS-2 for the marker-assisted selection of the high flesh/stone ratio. This strategy provides a rapid method for the characterization of RAPD markers and for the development of PCR-based markers with applications in olive mapping, paternal testing and germplasm characterization. The use of these markers in multiplexed PCRs, and the direct ethidium bromide detection of the PCR products in the test tube, facilitate their efficient and reliable breeding applications.
Main Authors: | Hernández Molina, Pilar, Rosa, Raúl de la, Rallo, Luis, Dorado, Gabriel, Martín, Antonio |
---|---|
Other Authors: | Comisión Interministerial de Ciencia y Tecnología, CICYT (España) |
Format: | artículo biblioteca |
Language: | English |
Published: |
Springer Nature
2001-10
|
Subjects: | Marker-assisted selection, Molecular breeding, Olea europaea, RAPD-PCR, Cultivar identification, |
Online Access: | http://hdl.handle.net/10261/184283 http://dx.doi.org/10.13039/501100007273 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Verticillium wilt of olive in Turkey: a survey on disease importance, pathogen diversity and susceptibility of relevant olive cultivars
by: Dervis, Sibel, et al.
Published: (2010-06) -
Origins and domestication of Mediterranean olive determined through RAPD marker analyses
by: Besnard, G., et al.
Published: (1998) -
Absolute quantification of olive oil DNA by droplet digital-PCR (ddPCR): Comparison of isolation and amplification methodologies
by: Scollo, Francesco, et al.
Published: (2016-12-15) -
Development of a new strategy for monitoring Epicoccum nigrum 282, a biological control agent used against brown rot caused by Monilinia spp. in peaches
by: Larena Nistal, María Inmaculada, et al.
Published: (2009) -
Use of molecular markers SSR and SCAR for identification of olive accessions.
by: VAL, A. D. B. do, et al.
Published: (2020-08-13)