Development of SCAR markers in olive (Olea europaea) by direct sequencing of RAPD products: applications in olive germplasm evaluation and mapping
RAPD markers generated by mixtures of two different 10-mer primers were developed for eight different olive cultivars used as parental lines in olive-breeding programs. Two RAPD bands were converted into dominant SCAR markers by direct sequencing of the RAPD products, avoiding the costly and time-consuming cloning step. The SCARs generated have maintained the original RAPD polymorphism among the cultivars and segregated according to Mendelian inheritance. Preliminary results suggest the use of the SCAR SCOeMS-2 for the marker-assisted selection of the high flesh/stone ratio. This strategy provides a rapid method for the characterization of RAPD markers and for the development of PCR-based markers with applications in olive mapping, paternal testing and germplasm characterization. The use of these markers in multiplexed PCRs, and the direct ethidium bromide detection of the PCR products in the test tube, facilitate their efficient and reliable breeding applications.
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Format: | artículo biblioteca |
Language: | English |
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Springer Nature
2001-10
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Subjects: | Marker-assisted selection, Molecular breeding, Olea europaea, RAPD-PCR, Cultivar identification, |
Online Access: | http://hdl.handle.net/10261/184283 http://dx.doi.org/10.13039/501100007273 |
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dig-ias-es-10261-1842832019-08-12T09:24:38Z Development of SCAR markers in olive (Olea europaea) by direct sequencing of RAPD products: applications in olive germplasm evaluation and mapping Hernández Molina, Pilar Rosa, Raúl de la Rallo, Luis Dorado, Gabriel Martín, Antonio Comisión Interministerial de Ciencia y Tecnología, CICYT (España) Marker-assisted selection Molecular breeding Olea europaea RAPD-PCR Cultivar identification RAPD markers generated by mixtures of two different 10-mer primers were developed for eight different olive cultivars used as parental lines in olive-breeding programs. Two RAPD bands were converted into dominant SCAR markers by direct sequencing of the RAPD products, avoiding the costly and time-consuming cloning step. The SCARs generated have maintained the original RAPD polymorphism among the cultivars and segregated according to Mendelian inheritance. Preliminary results suggest the use of the SCAR SCOeMS-2 for the marker-assisted selection of the high flesh/stone ratio. This strategy provides a rapid method for the characterization of RAPD markers and for the development of PCR-based markers with applications in olive mapping, paternal testing and germplasm characterization. The use of these markers in multiplexed PCRs, and the direct ethidium bromide detection of the PCR products in the test tube, facilitate their efficient and reliable breeding applications. Financial support from the CICYT andINIA, Spain (Projects OLI96-2184 and CAO98-001-C3-2), is acknowledged. Peer reviewed 2019-06-17T13:55:38Z 2019-06-17T13:55:38Z 2001-10 artículo http://purl.org/coar/resource_type/c_6501 Theoretical and Applied Genetics 103(5): 788-791 (2001) 0040-5752 http://hdl.handle.net/10261/184283 10.1007/s001220100603 1432-2242 http://dx.doi.org/10.13039/501100007273 en https://doi.org/10.1007/s001220100603 Sí none Springer Nature |
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Marker-assisted selection Molecular breeding Olea europaea RAPD-PCR Cultivar identification Marker-assisted selection Molecular breeding Olea europaea RAPD-PCR Cultivar identification |
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Marker-assisted selection Molecular breeding Olea europaea RAPD-PCR Cultivar identification Marker-assisted selection Molecular breeding Olea europaea RAPD-PCR Cultivar identification Hernández Molina, Pilar Rosa, Raúl de la Rallo, Luis Dorado, Gabriel Martín, Antonio Development of SCAR markers in olive (Olea europaea) by direct sequencing of RAPD products: applications in olive germplasm evaluation and mapping |
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RAPD markers generated by mixtures of two different 10-mer primers were developed for eight different olive cultivars used as parental lines in olive-breeding programs. Two RAPD bands were converted into dominant SCAR markers by direct sequencing of the RAPD products, avoiding the costly and time-consuming cloning step. The SCARs generated have maintained the original RAPD polymorphism among the cultivars and segregated according to Mendelian inheritance. Preliminary results suggest the use of the SCAR SCOeMS-2 for the marker-assisted selection of the high flesh/stone ratio. This strategy provides a rapid method for the characterization of RAPD markers and for the development of PCR-based markers with applications in olive mapping, paternal testing and germplasm characterization. The use of these markers in multiplexed PCRs, and the direct ethidium bromide detection of the PCR products in the test tube, facilitate their efficient and reliable breeding applications. |
author2 |
Comisión Interministerial de Ciencia y Tecnología, CICYT (España) |
author_facet |
Comisión Interministerial de Ciencia y Tecnología, CICYT (España) Hernández Molina, Pilar Rosa, Raúl de la Rallo, Luis Dorado, Gabriel Martín, Antonio |
format |
artículo |
topic_facet |
Marker-assisted selection Molecular breeding Olea europaea RAPD-PCR Cultivar identification |
author |
Hernández Molina, Pilar Rosa, Raúl de la Rallo, Luis Dorado, Gabriel Martín, Antonio |
author_sort |
Hernández Molina, Pilar |
title |
Development of SCAR markers in olive (Olea europaea) by direct sequencing of RAPD products: applications in olive germplasm evaluation and mapping |
title_short |
Development of SCAR markers in olive (Olea europaea) by direct sequencing of RAPD products: applications in olive germplasm evaluation and mapping |
title_full |
Development of SCAR markers in olive (Olea europaea) by direct sequencing of RAPD products: applications in olive germplasm evaluation and mapping |
title_fullStr |
Development of SCAR markers in olive (Olea europaea) by direct sequencing of RAPD products: applications in olive germplasm evaluation and mapping |
title_full_unstemmed |
Development of SCAR markers in olive (Olea europaea) by direct sequencing of RAPD products: applications in olive germplasm evaluation and mapping |
title_sort |
development of scar markers in olive (olea europaea) by direct sequencing of rapd products: applications in olive germplasm evaluation and mapping |
publisher |
Springer Nature |
publishDate |
2001-10 |
url |
http://hdl.handle.net/10261/184283 http://dx.doi.org/10.13039/501100007273 |
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