Targeting of prolamin by rnai in bread wheat
The present disclosure concerns the specific silencing of at least one of, but preferable all, of α (alpha), β (beta), γ (gamma), and ω (omega) gliadins and the LMW (low molecular weight) glutenin subunits of hard wheat and flour by interference RNA (iRNA) through use of a polynucleotide that is transcribed into an hpRNA (hairpin RNA). Furthermore, the present disclosure also concerns a vector, cell, plant, or seed comprising the polynucleotide. Expression can be specifically directed to particular tissues of wheat seeds through gene expression-regulating sequences such as, for example, the promoter of the gene that codes for a D-hordein.
Main Authors: | , |
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Format: | solicitud de patente biblioteca |
Language: | English |
Published: |
2016-12-22
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Online Access: | http://hdl.handle.net/10261/177096 |
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Summary: | The present disclosure concerns the specific silencing of at least one of, but preferable all, of α (alpha), β (beta), γ (gamma), and ω (omega) gliadins and the LMW (low molecular weight) glutenin subunits of hard wheat and flour by interference RNA (iRNA) through use of a polynucleotide that is transcribed into an hpRNA (hairpin RNA). Furthermore, the present disclosure also concerns a vector, cell, plant, or seed comprising the polynucleotide. Expression can be specifically directed to particular tissues of wheat seeds through gene expression-regulating sequences such as, for example, the promoter of the gene that codes for a D-hordein. |
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