Metabolomic characterization of xylem sap of different olive cultivars growing in Spain
Trabajo presentado en la European Conference on Xylella 2017 (Finding answers to a global problem), celebrada en Palma de Mallorca del 13 al 15 de noviembre de 2017.
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2017-11
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dig-ias-es-10261-1679582018-08-03T07:54:35Z Metabolomic characterization of xylem sap of different olive cultivars growing in Spain Montes Borrego, Miguel Jiménez-Díaz, Rafael M. Trapero Casas, José Luis Navas Cortés, Juan Antonio Haro, Carmen Rivas, J. C. Fuente, L. de la Landa, Blanca B. Ministerio de Economía, Industria y Competitividad (España) European Commission Trabajo presentado en la European Conference on Xylella 2017 (Finding answers to a global problem), celebrada en Palma de Mallorca del 13 al 15 de noviembre de 2017. With the goal of understanding the nutritional requirements of xylem-limited microbiome, the chemical composition of xylem sap extracted from two Olea europaea var. europaea cultivars (Picual and Arbequina) was evaluated. Selected plants were established on their own feet or grafted onto rootstocks of Olea europaea var. sylvestris. Xylem sap of olives branches was collected using a external 45-cm external Scholander Super Chamber. Metabolome analysis was performed by proton nuclear magnetic resonance (NMR) spectroscopy-based study and the ionome analysis was performed by inductively coupled plasma with optical emission spectroscopy (ICP-OES). In total 26 metabolites were identified by NMR spectroscopy, including amino acids (alanine, arginine, aspartate, glutamate, glutamine, isoleucine, leucine, methionine, proline, threonine, tyrosine, and valine), sugars (glucose, fructose, mannitol, myo-inositol, and sucrose), organic acids (formic, fumaric and succinic acid), alcohols (ethanol and methanol), and other molecules. On the other hand ICP-OES allowed the quantification of 14 elements and five inorganic anions. Xylem sap analysis will lead to a better understanding of the biology and complex nutritional requirements of olive xylem-inhabiting microorganisms, including Xylella fastidiosa, and to help designing artificial growing media to improve culturing of the olive microbiome. Study supported by Projects 727987 XF-ACTORS (EU-H2020) & AGL2016-75606-R (MEIC Spain and FEDER-EU). No 2018-07-26T09:34:21Z 2018-07-26T09:34:21Z 2017-11 póster de congreso http://purl.org/coar/resource_type/c_6670 European Conference on Xylella (2017) http://hdl.handle.net/10261/167958 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100010198 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/H2020/727987 info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2016-75606-R Sí none |
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Trabajo presentado en la European Conference on Xylella 2017 (Finding answers to a global problem), celebrada en Palma de Mallorca del 13 al 15 de noviembre de 2017. |
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Ministerio de Economía, Industria y Competitividad (España) |
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Ministerio de Economía, Industria y Competitividad (España) Montes Borrego, Miguel Jiménez-Díaz, Rafael M. Trapero Casas, José Luis Navas Cortés, Juan Antonio Haro, Carmen Rivas, J. C. Fuente, L. de la Landa, Blanca B. |
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póster de congreso |
author |
Montes Borrego, Miguel Jiménez-Díaz, Rafael M. Trapero Casas, José Luis Navas Cortés, Juan Antonio Haro, Carmen Rivas, J. C. Fuente, L. de la Landa, Blanca B. |
spellingShingle |
Montes Borrego, Miguel Jiménez-Díaz, Rafael M. Trapero Casas, José Luis Navas Cortés, Juan Antonio Haro, Carmen Rivas, J. C. Fuente, L. de la Landa, Blanca B. Metabolomic characterization of xylem sap of different olive cultivars growing in Spain |
author_sort |
Montes Borrego, Miguel |
title |
Metabolomic characterization of xylem sap of different olive cultivars growing in Spain |
title_short |
Metabolomic characterization of xylem sap of different olive cultivars growing in Spain |
title_full |
Metabolomic characterization of xylem sap of different olive cultivars growing in Spain |
title_fullStr |
Metabolomic characterization of xylem sap of different olive cultivars growing in Spain |
title_full_unstemmed |
Metabolomic characterization of xylem sap of different olive cultivars growing in Spain |
title_sort |
metabolomic characterization of xylem sap of different olive cultivars growing in spain |
publishDate |
2017-11 |
url |
http://hdl.handle.net/10261/167958 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100010198 |
work_keys_str_mv |
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