Physical and Chemical Barriers in Root Tissues Contribute to Quantitative Resistance to Fusarium oxysporum f. sp. pisi in Pea
Fusarium wilt caused by Fusarium oxysporum f. sp. pisi (Fop) is one of the most destructive diseases of pea worldwide. Control of this disease is difficult and it is mainly based on the use of resistant cultivars. While monogenic resistance has been successfully used in the field, it is at risk of breakdown by the constant evolution of the pathogen. New sources of quantitative resistance have been recently identified from a wild relative Pisum spp. collection. Here, we characterize histologically the resistance mechanisms occurring in these sources of quantitative resistance. Detailed comparison, of the reaction at cellular level, of eight pea accessions with differential responses to Fop race 2, showed that resistant accessions established several barriers at the epidermis, exodermis, cortex, endodermis and vascular stele efficiently impeding fungal progression. The main components of these different barriers were carbohydrates and phenolic compounds including lignin. We found that these barriers were mainly based on three defense mechanisms including cell wall strengthening, formation of papilla-like structures at penetration sites and accumulation of different substances within and between cells. These defense reactions varied in intensity and localization between resistant accessions. Our results also clarify some steps of the infection process of F. oxysporum in plant and support the important role of cell wall-degrading enzymes in F. oxysporum pathogenicity.
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Language: | English |
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Frontiers Media
2018-02-19
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Subjects: | Cell wall strengthening, Disease resistance, Fusarium oxysporum, Papilla-like structure, Phenolic compounds, Pisum sativum, Quantitative resistance, Resistance mechanism, |
Online Access: | http://hdl.handle.net/10261/166977 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100010198 |
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dig-ias-es-10261-1669772021-12-28T16:36:59Z Physical and Chemical Barriers in Root Tissues Contribute to Quantitative Resistance to Fusarium oxysporum f. sp. pisi in Pea Bani, Moustapha Pérez de Luque, Alejandro Rubiales, Diego Rispail, Nicolas Ministerio de Economía, Industria y Competitividad (España) European Commission Consejo Superior de Investigaciones Científicas (España) Cell wall strengthening Disease resistance Fusarium oxysporum Papilla-like structure Phenolic compounds Pisum sativum Quantitative resistance Resistance mechanism Fusarium wilt caused by Fusarium oxysporum f. sp. pisi (Fop) is one of the most destructive diseases of pea worldwide. Control of this disease is difficult and it is mainly based on the use of resistant cultivars. While monogenic resistance has been successfully used in the field, it is at risk of breakdown by the constant evolution of the pathogen. New sources of quantitative resistance have been recently identified from a wild relative Pisum spp. collection. Here, we characterize histologically the resistance mechanisms occurring in these sources of quantitative resistance. Detailed comparison, of the reaction at cellular level, of eight pea accessions with differential responses to Fop race 2, showed that resistant accessions established several barriers at the epidermis, exodermis, cortex, endodermis and vascular stele efficiently impeding fungal progression. The main components of these different barriers were carbohydrates and phenolic compounds including lignin. We found that these barriers were mainly based on three defense mechanisms including cell wall strengthening, formation of papilla-like structures at penetration sites and accumulation of different substances within and between cells. These defense reactions varied in intensity and localization between resistant accessions. Our results also clarify some steps of the infection process of F. oxysporum in plant and support the important role of cell wall-degrading enzymes in F. oxysporum pathogenicity. This work was supported by the Spanish National Projects (AGL2014-52871-R and AGL2017-82907-R) from the Spanish Ministry of Economy, Industry and Competitiveness (MINEICO) and co-financed by European Fund for Regional Development (FEDER), the European KBBE Project LEGATO [FP7-KBBE2013.1.2-02-613551], and the I-COOP AGROFOOD 2014 action (Project No. 2014CD0011) of the Spanish National Research Council (CSIC). NR is holder of a Ramón y Cajal Postdoctoral position from MINECO. Peer reviewed 2018-06-25T11:48:12Z 2018-06-25T11:48:12Z 2018-02-19 artículo http://purl.org/coar/resource_type/c_6501 Frontiers in Plant Science 9: 199 (2018) http://hdl.handle.net/10261/166977 10.3389/fpls.2018.00199 1664-462X http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100010198 29515610 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-52871-R info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-82907-R info:eu-repo/grantAgreement/EC/FP7/613551 Publisher's version http://dx.doi.org/10.3389/fpls.2018.00199 Sí open Frontiers Media |
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Cell wall strengthening Disease resistance Fusarium oxysporum Papilla-like structure Phenolic compounds Pisum sativum Quantitative resistance Resistance mechanism Cell wall strengthening Disease resistance Fusarium oxysporum Papilla-like structure Phenolic compounds Pisum sativum Quantitative resistance Resistance mechanism |
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Cell wall strengthening Disease resistance Fusarium oxysporum Papilla-like structure Phenolic compounds Pisum sativum Quantitative resistance Resistance mechanism Cell wall strengthening Disease resistance Fusarium oxysporum Papilla-like structure Phenolic compounds Pisum sativum Quantitative resistance Resistance mechanism Bani, Moustapha Pérez de Luque, Alejandro Rubiales, Diego Rispail, Nicolas Physical and Chemical Barriers in Root Tissues Contribute to Quantitative Resistance to Fusarium oxysporum f. sp. pisi in Pea |
description |
Fusarium wilt caused by Fusarium oxysporum f. sp. pisi (Fop) is one of the most destructive diseases of pea worldwide. Control of this disease is difficult and it is mainly based on the use of resistant cultivars. While monogenic resistance has been successfully used in the field, it is at risk of breakdown by the constant evolution of the pathogen. New sources of quantitative resistance have been recently identified from a wild relative Pisum spp. collection. Here, we characterize histologically the resistance mechanisms occurring in these sources of quantitative resistance. Detailed comparison, of the reaction at cellular level, of eight pea accessions with differential responses to Fop race 2, showed that resistant accessions established several barriers at the epidermis, exodermis, cortex, endodermis and vascular stele efficiently impeding fungal progression. The main components of these different barriers were carbohydrates and phenolic compounds including lignin. We found that these barriers were mainly based on three defense mechanisms including cell wall strengthening, formation of papilla-like structures at penetration sites and accumulation of different substances within and between cells. These defense reactions varied in intensity and localization between resistant accessions. Our results also clarify some steps of the infection process of F. oxysporum in plant and support the important role of cell wall-degrading enzymes in F. oxysporum pathogenicity. |
author2 |
Ministerio de Economía, Industria y Competitividad (España) |
author_facet |
Ministerio de Economía, Industria y Competitividad (España) Bani, Moustapha Pérez de Luque, Alejandro Rubiales, Diego Rispail, Nicolas |
format |
artículo |
topic_facet |
Cell wall strengthening Disease resistance Fusarium oxysporum Papilla-like structure Phenolic compounds Pisum sativum Quantitative resistance Resistance mechanism |
author |
Bani, Moustapha Pérez de Luque, Alejandro Rubiales, Diego Rispail, Nicolas |
author_sort |
Bani, Moustapha |
title |
Physical and Chemical Barriers in Root Tissues Contribute to Quantitative Resistance to Fusarium oxysporum f. sp. pisi in Pea |
title_short |
Physical and Chemical Barriers in Root Tissues Contribute to Quantitative Resistance to Fusarium oxysporum f. sp. pisi in Pea |
title_full |
Physical and Chemical Barriers in Root Tissues Contribute to Quantitative Resistance to Fusarium oxysporum f. sp. pisi in Pea |
title_fullStr |
Physical and Chemical Barriers in Root Tissues Contribute to Quantitative Resistance to Fusarium oxysporum f. sp. pisi in Pea |
title_full_unstemmed |
Physical and Chemical Barriers in Root Tissues Contribute to Quantitative Resistance to Fusarium oxysporum f. sp. pisi in Pea |
title_sort |
physical and chemical barriers in root tissues contribute to quantitative resistance to fusarium oxysporum f. sp. pisi in pea |
publisher |
Frontiers Media |
publishDate |
2018-02-19 |
url |
http://hdl.handle.net/10261/166977 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100010198 |
work_keys_str_mv |
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