Morphological and molecular characterisation of Helicotylenchus pseudorobustus (Steiner, 1914) Golden, 1956 and related species (Tylenchida: Hoplolaimidae) with a phylogeny of the genus
Morphological identification of spiral nematodes of the genus Helicotylenchus is a difficult task because most characters used for their diagnosis vary within species. In this paper we provide morphological and molecular characterisations of several spiral nematodes, H. broadbalkiensis, H. digonicus, H. dihystera, H. microlobus, H. paxilli and H. pseudorobustus, collected in different geographical areas of USA, Switzerland, Italy, New Zealand, Spain, UK, South Korea and Russia. We suggest that H. microlobus and H. pseudorobustus are valid species separated from each other morphologically and molecularly. Seven species with distinct molecular characteristics are also distinguished, but are not ascribed morphologically to any specific taxon because of the low number of specimens available. Phylogenetic relationships of H. pseudorobustus with other Helicotylenchus species are given as inferred from the analyses of 154 sequences of the D2-D3 of 28S rRNA gene and 37 sequences of ITS rRNA gene.
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| Format: | artículo biblioteca |
| Language: | English |
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Brill Academic Publishers
2015
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| Subjects: | Taxonomy, Spiral nematodes, Morphology, 28S rRNA gene, Morphometrics, Helicotylenchus depressus, Helicotylenchus digonicus, Helicotylenchus paxilli, Helicotylenchus broadbalkiensis, ITS rRNA gene, Helicotylenchus microlobus, |
| Online Access: | http://hdl.handle.net/10261/158461 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100002261 http://dx.doi.org/10.13039/501100011011 |
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dig-ias-es-10261-1584612018-08-01T10:05:02Z Morphological and molecular characterisation of Helicotylenchus pseudorobustus (Steiner, 1914) Golden, 1956 and related species (Tylenchida: Hoplolaimidae) with a phylogeny of the genus Subbotin, Sergei A. Vovlas, Nicola Yeates, Gregor W. Hallmann, Johannes Kiewnick, Sebastian Chizhov, Vladimir N. Manzanilla-López, Rosa H. Inserra, Renato N. Castillo, Pablo Ministerio de Economía y Competitividad (España) Junta de Andalucía European Commission Russian Foundation for Basic Research Taxonomy Spiral nematodes Morphology 28S rRNA gene Morphometrics Helicotylenchus depressus Helicotylenchus digonicus Helicotylenchus paxilli Helicotylenchus broadbalkiensis ITS rRNA gene Helicotylenchus microlobus Morphological identification of spiral nematodes of the genus Helicotylenchus is a difficult task because most characters used for their diagnosis vary within species. In this paper we provide morphological and molecular characterisations of several spiral nematodes, H. broadbalkiensis, H. digonicus, H. dihystera, H. microlobus, H. paxilli and H. pseudorobustus, collected in different geographical areas of USA, Switzerland, Italy, New Zealand, Spain, UK, South Korea and Russia. We suggest that H. microlobus and H. pseudorobustus are valid species separated from each other morphologically and molecularly. Seven species with distinct molecular characteristics are also distinguished, but are not ascribed morphologically to any specific taxon because of the low number of specimens available. Phylogenetic relationships of H. pseudorobustus with other Helicotylenchus species are given as inferred from the analyses of 154 sequences of the D2-D3 of 28S rRNA gene and 37 sequences of ITS rRNA gene. This research was partially supported by grant AGL2012-37521 from ‘Ministerio de Economía y Competitividad’ of Spain, grant P12-AGR 1486 and grant AGR-136 from ‘Consejería de Economía, Innvovación y Ciencia’ from Junta de Andalucía and Union Europea, Fondo Europeo de Desarrollo regional, ‘Una manera de hacer Europa’, and the Russian Foundation of Basic Research, project number 14-04-00953. Peer reviewed 2017-12-21T14:28:49Z 2017-12-21T14:28:49Z 2015 artículo http://purl.org/coar/resource_type/c_6501 Nematology 17(1): 27-52 (2015) 1388-5545 http://hdl.handle.net/10261/158461 10.1163/15685411-00002850 1568-5411 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100002261 http://dx.doi.org/10.13039/501100011011 en http://doi.org/10.1163/15685411-00002850 Sí none Brill Academic Publishers |
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| databasecode |
dig-ias-es |
| tag |
biblioteca |
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Europa del Sur |
| libraryname |
Biblioteca del IAS España |
| language |
English |
| topic |
Taxonomy Spiral nematodes Morphology 28S rRNA gene Morphometrics Helicotylenchus depressus Helicotylenchus digonicus Helicotylenchus paxilli Helicotylenchus broadbalkiensis ITS rRNA gene Helicotylenchus microlobus Taxonomy Spiral nematodes Morphology 28S rRNA gene Morphometrics Helicotylenchus depressus Helicotylenchus digonicus Helicotylenchus paxilli Helicotylenchus broadbalkiensis ITS rRNA gene Helicotylenchus microlobus |
| spellingShingle |
Taxonomy Spiral nematodes Morphology 28S rRNA gene Morphometrics Helicotylenchus depressus Helicotylenchus digonicus Helicotylenchus paxilli Helicotylenchus broadbalkiensis ITS rRNA gene Helicotylenchus microlobus Taxonomy Spiral nematodes Morphology 28S rRNA gene Morphometrics Helicotylenchus depressus Helicotylenchus digonicus Helicotylenchus paxilli Helicotylenchus broadbalkiensis ITS rRNA gene Helicotylenchus microlobus Subbotin, Sergei A. Vovlas, Nicola Yeates, Gregor W. Hallmann, Johannes Kiewnick, Sebastian Chizhov, Vladimir N. Manzanilla-López, Rosa H. Inserra, Renato N. Castillo, Pablo Morphological and molecular characterisation of Helicotylenchus pseudorobustus (Steiner, 1914) Golden, 1956 and related species (Tylenchida: Hoplolaimidae) with a phylogeny of the genus |
| description |
Morphological identification of spiral nematodes of the genus Helicotylenchus is a difficult task because most characters used for their diagnosis vary within species. In this paper we provide morphological and molecular characterisations of several spiral nematodes, H. broadbalkiensis, H. digonicus, H. dihystera, H. microlobus, H. paxilli and H. pseudorobustus, collected in different geographical areas of USA, Switzerland, Italy, New Zealand, Spain, UK, South Korea and Russia. We suggest that H. microlobus and H. pseudorobustus are valid species separated from each other morphologically and molecularly. Seven species with distinct molecular characteristics are also distinguished, but are not ascribed morphologically to any specific taxon because of the low number of specimens available. Phylogenetic relationships of H. pseudorobustus with other Helicotylenchus species are given as inferred from the analyses of 154 sequences of the D2-D3 of 28S rRNA gene and 37 sequences of ITS rRNA gene. |
| author2 |
Ministerio de Economía y Competitividad (España) |
| author_facet |
Ministerio de Economía y Competitividad (España) Subbotin, Sergei A. Vovlas, Nicola Yeates, Gregor W. Hallmann, Johannes Kiewnick, Sebastian Chizhov, Vladimir N. Manzanilla-López, Rosa H. Inserra, Renato N. Castillo, Pablo |
| format |
artículo |
| topic_facet |
Taxonomy Spiral nematodes Morphology 28S rRNA gene Morphometrics Helicotylenchus depressus Helicotylenchus digonicus Helicotylenchus paxilli Helicotylenchus broadbalkiensis ITS rRNA gene Helicotylenchus microlobus |
| author |
Subbotin, Sergei A. Vovlas, Nicola Yeates, Gregor W. Hallmann, Johannes Kiewnick, Sebastian Chizhov, Vladimir N. Manzanilla-López, Rosa H. Inserra, Renato N. Castillo, Pablo |
| author_sort |
Subbotin, Sergei A. |
| title |
Morphological and molecular characterisation of Helicotylenchus pseudorobustus (Steiner, 1914) Golden, 1956 and related species (Tylenchida: Hoplolaimidae) with a phylogeny of the genus |
| title_short |
Morphological and molecular characterisation of Helicotylenchus pseudorobustus (Steiner, 1914) Golden, 1956 and related species (Tylenchida: Hoplolaimidae) with a phylogeny of the genus |
| title_full |
Morphological and molecular characterisation of Helicotylenchus pseudorobustus (Steiner, 1914) Golden, 1956 and related species (Tylenchida: Hoplolaimidae) with a phylogeny of the genus |
| title_fullStr |
Morphological and molecular characterisation of Helicotylenchus pseudorobustus (Steiner, 1914) Golden, 1956 and related species (Tylenchida: Hoplolaimidae) with a phylogeny of the genus |
| title_full_unstemmed |
Morphological and molecular characterisation of Helicotylenchus pseudorobustus (Steiner, 1914) Golden, 1956 and related species (Tylenchida: Hoplolaimidae) with a phylogeny of the genus |
| title_sort |
morphological and molecular characterisation of helicotylenchus pseudorobustus (steiner, 1914) golden, 1956 and related species (tylenchida: hoplolaimidae) with a phylogeny of the genus |
| publisher |
Brill Academic Publishers |
| publishDate |
2015 |
| url |
http://hdl.handle.net/10261/158461 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100002261 http://dx.doi.org/10.13039/501100011011 |
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| _version_ |
1777663134692016128 |