Genetic Diversity Assessment of Portuguese Cultivated Vicia faba L. through IRAP Markers
Faba bean have been grown in Portugal for a long time and locally adapted populations are still maintained on farm. The genetic diversity of four Portuguese faba bean populations that are still cultivated in some regions of the country was evaluated using the Inter Retrotransposons Amplified Polymorphism (IRAP) technique. It was shown that molecular markers based on retrotransposons previously identified in other species can be efficiently used in the genetic variability assessment of Vicia faba. The IRAP experiment targeting Athila yielded the most informative banding patterns. Cluster analysis using the neighbor-joining algorithm generated a dendrogram that clearly shows the distribution pattern of V. faba samples. The four equina accessions are separated from each other and form two distinct clades while the two major faba bean accessions are not unequivocally separated by the IRAP. Fluorescent In Situ Hybridization (FISH) analysis of sequences amplified by IRAP Athila revealed a wide distribution throughout V. faba chromosomes, confirming the whole-genome coverage of this molecular marker. Morphological characteristics were also assessed through cluster analysis of seed characters using the unweighted pair group method arithmetic average (UPGMA) and principal component analysis (PCA), showing a clear discrimination between faba bean major and equina groups. It was also found that the seed character most relevant to distinguish accessions was 100 seed weight. Seed morphological traits and IRAP evaluation give similar results supporting the potential of IRAP analysis for genetic diversity studies.
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Molecular Diversity Preservation International
2016-03-25
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Subjects: | Faba bean locally adapted populations, Genomic diversity, IRAP, |
Online Access: | http://hdl.handle.net/10261/157868 http://dx.doi.org/10.13039/501100001871 |
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dig-ias-es-10261-1578682017-12-02T01:54:34Z Genetic Diversity Assessment of Portuguese Cultivated Vicia faba L. through IRAP Markers Tomás, Diana Dias, Ana Luisa Silva, Manuela Oliveira, Hugo R. Suso Llamas, María José Viegas, Wanda Veloso, Maria Manuela Fundação para a Ciência e a Tecnologia (Portugal) Faba bean locally adapted populations Genomic diversity IRAP Faba bean have been grown in Portugal for a long time and locally adapted populations are still maintained on farm. The genetic diversity of four Portuguese faba bean populations that are still cultivated in some regions of the country was evaluated using the Inter Retrotransposons Amplified Polymorphism (IRAP) technique. It was shown that molecular markers based on retrotransposons previously identified in other species can be efficiently used in the genetic variability assessment of Vicia faba. The IRAP experiment targeting Athila yielded the most informative banding patterns. Cluster analysis using the neighbor-joining algorithm generated a dendrogram that clearly shows the distribution pattern of V. faba samples. The four equina accessions are separated from each other and form two distinct clades while the two major faba bean accessions are not unequivocally separated by the IRAP. Fluorescent In Situ Hybridization (FISH) analysis of sequences amplified by IRAP Athila revealed a wide distribution throughout V. faba chromosomes, confirming the whole-genome coverage of this molecular marker. Morphological characteristics were also assessed through cluster analysis of seed characters using the unweighted pair group method arithmetic average (UPGMA) and principal component analysis (PCA), showing a clear discrimination between faba bean major and equina groups. It was also found that the seed character most relevant to distinguish accessions was 100 seed weight. Seed morphological traits and IRAP evaluation give similar results supporting the potential of IRAP analysis for genetic diversity studies. Diana Tomás was funded by a doctoral scholarship (SFRH/BD/93156/2013) by Fundação para a Ciência e a Tecnologia (FCT), Portugal and the research work was financed by the FCT LEAF Unit (UID/AGR/04129/2013). Peer reviewed 2017-11-29T13:45:09Z 2017-11-29T13:45:09Z 2016-03-25 artículo http://purl.org/coar/resource_type/c_6501 Diversity 8: 8 (2016) 1424-2818 http://hdl.handle.net/10261/157868 10.3390/d8020008 http://dx.doi.org/10.13039/501100001871 en Publisher's version http://doi.org/10.3390/d8020008 Sí open Molecular Diversity Preservation International |
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Faba bean locally adapted populations Genomic diversity IRAP Faba bean locally adapted populations Genomic diversity IRAP Tomás, Diana Dias, Ana Luisa Silva, Manuela Oliveira, Hugo R. Suso Llamas, María José Viegas, Wanda Veloso, Maria Manuela Genetic Diversity Assessment of Portuguese Cultivated Vicia faba L. through IRAP Markers |
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Faba bean have been grown in Portugal for a long time and locally adapted populations are still maintained on farm. The genetic diversity of four Portuguese faba bean populations that are still cultivated in some regions of the country was evaluated using the Inter Retrotransposons Amplified Polymorphism (IRAP) technique. It was shown that molecular markers based on retrotransposons previously identified in other species can be efficiently used in the genetic variability assessment of Vicia faba. The IRAP experiment targeting Athila yielded the most informative banding patterns. Cluster analysis using the neighbor-joining algorithm generated a dendrogram that clearly shows the distribution pattern of V. faba samples. The four equina accessions are separated from each other and form two distinct clades while the two major faba bean accessions are not unequivocally separated by the IRAP. Fluorescent In Situ Hybridization (FISH) analysis of sequences amplified by IRAP Athila revealed a wide distribution throughout V. faba chromosomes, confirming the whole-genome coverage of this molecular marker. Morphological characteristics were also assessed through cluster analysis of seed characters using the unweighted pair group method arithmetic average (UPGMA) and principal component analysis (PCA), showing a clear discrimination between faba bean major and equina groups. It was also found that the seed character most relevant to distinguish accessions was 100 seed weight. Seed morphological traits and IRAP evaluation give similar results supporting the potential of IRAP analysis for genetic diversity studies. |
author2 |
Fundação para a Ciência e a Tecnologia (Portugal) |
author_facet |
Fundação para a Ciência e a Tecnologia (Portugal) Tomás, Diana Dias, Ana Luisa Silva, Manuela Oliveira, Hugo R. Suso Llamas, María José Viegas, Wanda Veloso, Maria Manuela |
format |
artículo |
topic_facet |
Faba bean locally adapted populations Genomic diversity IRAP |
author |
Tomás, Diana Dias, Ana Luisa Silva, Manuela Oliveira, Hugo R. Suso Llamas, María José Viegas, Wanda Veloso, Maria Manuela |
author_sort |
Tomás, Diana |
title |
Genetic Diversity Assessment of Portuguese Cultivated Vicia faba L. through IRAP Markers |
title_short |
Genetic Diversity Assessment of Portuguese Cultivated Vicia faba L. through IRAP Markers |
title_full |
Genetic Diversity Assessment of Portuguese Cultivated Vicia faba L. through IRAP Markers |
title_fullStr |
Genetic Diversity Assessment of Portuguese Cultivated Vicia faba L. through IRAP Markers |
title_full_unstemmed |
Genetic Diversity Assessment of Portuguese Cultivated Vicia faba L. through IRAP Markers |
title_sort |
genetic diversity assessment of portuguese cultivated vicia faba l. through irap markers |
publisher |
Molecular Diversity Preservation International |
publishDate |
2016-03-25 |
url |
http://hdl.handle.net/10261/157868 http://dx.doi.org/10.13039/501100001871 |
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