Characterization of resistance against the olive-defoliating Verticillium dahliae pathotype in selected clones of wild olive
Verticillium wilt of olive is best managed by resistant cultivars, but those currently available show incomplete resistance to the defoliating (D) Verticillium dahliae pathotype. Moreover, these cultivars do not satisfy consumers' demand for high yields and oil quality. Highly resistant rootstocks would be of paramount importance for production of agronomically adapted and commercially desirable olive cultivars in D V. dahliae-infested soils. In this work, resistance to D V. dahliae in wild olive clones Ac-13, Ac-18, OutVert and StopVert was assessed by quantifying the fungal DNA along the stem using a highly sensitive real-time quantitative polymerase chain reaction (qPCR) protocol and a stem colonization index (SCI) based on isolation of V. dahliae following artificial inoculations under conditions highly conducive for verticillium wilt. Ac-13, Ac-18, OutVert and StopVert showed a symptomless reaction to D V. dahliae. The mean amount of D V. dahliaeDNA quantified in stems of the four clones ranged from 3.64 to 28.89 pg/100 ng olive DNA, which was 249 to 1537 times lower than that in susceptible Picual olive. The reduction in the quantitative stem colonization of wild olive clones by D V. dahliae was also indicated by a sharp decrease in the SCI. Overall, there was a pattern of decreasing SCI in acropetal progression along the plant axis, as well as correlation between positive reisolation and quantification of pathogen DNA. The results of this research show that wild olive clones Ac-13, Ac-18, OutVert and StopVert have a valuable potential as rootstocks for the management of verticillium wilt in olive.
Main Authors: | Jiménez Fernández, Daniel, Trapero Casas, José Luis, Landa, Blanca B., Navas Cortés, Juan Antonio, Bubici, Giovanni, Cirulli, Matteo, Jiménez-Díaz, Rafael M. |
---|---|
Other Authors: | European Commission |
Format: | artículo biblioteca |
Language: | English |
Published: |
John Wiley & Sons
2016-10
|
Subjects: | Olea europaea L. subsp. europaea var. sylvestris, Olive, qPCR, Rootstocks, Verticillium wilt, |
Online Access: | http://hdl.handle.net/10261/157671 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100011011 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Microbial communities associated with the root system of wild olives (Olea europaea L. subsp. europaea var. sylvestris) are good reservoirs of bacteria with antagonistic potential against Verticillium dahliae
by: Aranda Ocampo, Sergio, et al.
Published: (2011-06) -
Wild Olive Genotypes as a Valuable Source of Resistance to Defoliating Verticillium dahliae
by: Díaz-Rueda, P., et al.
Published: (2021-07-01) -
Infection by Meloidogyne javanica does not breakdown resistance to the defoliating pathotype of Verticillium dahliae in selected clones of wild olive
by: Palomares Rius, Juan E., et al.
Published: (2016-02-16) -
Variation of pathotypes and races and their correlations with clonal lineages in Verticillium dahliae
by: Jiménez-Díaz, Rafael M., et al.
Published: (2017-05) -
Avoidant/resistant rather than tolerant olive rootstocks are more effective in controlling Verticillium wilt
by: Díaz-Rueda, P., et al.
Published: (2022-10-17)