Molecular characterization and distribution of the needle nematode Longidorus laevicapitatus Williams, 1959 (Nematoda: Longidoridae) in Costa Rica
Correct identification of Longidorus species in Costa Rica is essential to establish appropriate control strategies for preventing the spread of these nematodes. Nematode surveys conducted in the rainy seasons from 2013 to 2015 in areas arbitrarily chosen and widely distributed in the whole territory of Costa Rica, resulted in an overall prevalence of Longidorus spp. infesting soils both cultivated, ornamental and wild plants of 40.26 %. Integrative morphometric and molecular data for Longidorus populations were obtained using D2-D3 expansion segments of 28S rRNA, ITS1-rRNA, and the partial 18S-rRNA, identifying a solely species identified as Longidorus laevicapitatus. Morphology and morphometrical traits analysis of these populations of L. laevicapitatus were in agreement with those of the original and posterior descriptions of the species, except for some minor differences, which may be a result of intraspecific variability. The phylogenetic relationships of this species with other representatives of Longidorus spp. using D2-D3 expansion segments and the partial 18S indicated that L. laevicapitatus clustered clearly separately in a basal position in both phylogenetic trees.
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Language: | English |
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Springer
2017-02
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Subjects: | Bayesian inference, D2-D3, ITS1, 18S, Longidorids, Phylogeny, rDNA, Taxonomy, |
Online Access: | http://hdl.handle.net/10261/157199 |
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dig-ias-es-10261-1571992018-08-01T10:05:02Z Molecular characterization and distribution of the needle nematode Longidorus laevicapitatus Williams, 1959 (Nematoda: Longidoridae) in Costa Rica Peraza-Padilla, Walter Archidona-Yuste, Antonio Cantalapiedra-Navarrete, C. Zamora-Araya, Tatiana Palomares Rius, Juan E. Castillo, Pablo Universidad Nacional de Costa Rica Bayesian inference D2-D3 ITS1 18S Longidorids Phylogeny rDNA Taxonomy Correct identification of Longidorus species in Costa Rica is essential to establish appropriate control strategies for preventing the spread of these nematodes. Nematode surveys conducted in the rainy seasons from 2013 to 2015 in areas arbitrarily chosen and widely distributed in the whole territory of Costa Rica, resulted in an overall prevalence of Longidorus spp. infesting soils both cultivated, ornamental and wild plants of 40.26 %. Integrative morphometric and molecular data for Longidorus populations were obtained using D2-D3 expansion segments of 28S rRNA, ITS1-rRNA, and the partial 18S-rRNA, identifying a solely species identified as Longidorus laevicapitatus. Morphology and morphometrical traits analysis of these populations of L. laevicapitatus were in agreement with those of the original and posterior descriptions of the species, except for some minor differences, which may be a result of intraspecific variability. The phylogenetic relationships of this species with other representatives of Longidorus spp. using D2-D3 expansion segments and the partial 18S indicated that L. laevicapitatus clustered clearly separately in a basal position in both phylogenetic trees. Research was supported by grant of Universidad Nacional and FIDA (Fondo Institucional al Desarrollo Académico) from Costa Rica. Peer reviewed 2017-11-10T12:01:10Z 2017-11-10T12:01:10Z 2017-02 artículo http://purl.org/coar/resource_type/c_6501 European Journal of Plant Pathology 147(2): 443–450 (2017) 0929-1873 http://hdl.handle.net/10261/157199 10.1007/s10658-016-0997-4 1573-8469 en http://doi.org/10.1007/s10658-016-0997-4 Sí none Springer |
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Bayesian inference D2-D3 ITS1 18S Longidorids Phylogeny rDNA Taxonomy Bayesian inference D2-D3 ITS1 18S Longidorids Phylogeny rDNA Taxonomy |
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Bayesian inference D2-D3 ITS1 18S Longidorids Phylogeny rDNA Taxonomy Bayesian inference D2-D3 ITS1 18S Longidorids Phylogeny rDNA Taxonomy Peraza-Padilla, Walter Archidona-Yuste, Antonio Cantalapiedra-Navarrete, C. Zamora-Araya, Tatiana Palomares Rius, Juan E. Castillo, Pablo Molecular characterization and distribution of the needle nematode Longidorus laevicapitatus Williams, 1959 (Nematoda: Longidoridae) in Costa Rica |
description |
Correct identification of Longidorus species in Costa Rica is essential to establish appropriate control strategies for preventing the spread of these nematodes. Nematode surveys conducted in the rainy seasons from 2013 to 2015 in areas arbitrarily chosen and widely distributed in the whole territory of Costa Rica, resulted in an overall prevalence of Longidorus spp. infesting soils both cultivated, ornamental and wild plants of 40.26 %. Integrative morphometric and molecular data for Longidorus populations were obtained using D2-D3 expansion segments of 28S rRNA, ITS1-rRNA, and the partial 18S-rRNA, identifying a solely species identified as Longidorus laevicapitatus. Morphology and morphometrical traits analysis of these populations of L. laevicapitatus were in agreement with those of the original and posterior descriptions of the species, except for some minor differences, which may be a result of intraspecific variability. The phylogenetic relationships of this species with other representatives of Longidorus spp. using D2-D3 expansion segments and the partial 18S indicated that L. laevicapitatus clustered clearly separately in a basal position in both phylogenetic trees. |
author2 |
Universidad Nacional de Costa Rica |
author_facet |
Universidad Nacional de Costa Rica Peraza-Padilla, Walter Archidona-Yuste, Antonio Cantalapiedra-Navarrete, C. Zamora-Araya, Tatiana Palomares Rius, Juan E. Castillo, Pablo |
format |
artículo |
topic_facet |
Bayesian inference D2-D3 ITS1 18S Longidorids Phylogeny rDNA Taxonomy |
author |
Peraza-Padilla, Walter Archidona-Yuste, Antonio Cantalapiedra-Navarrete, C. Zamora-Araya, Tatiana Palomares Rius, Juan E. Castillo, Pablo |
author_sort |
Peraza-Padilla, Walter |
title |
Molecular characterization and distribution of the needle nematode Longidorus laevicapitatus Williams, 1959 (Nematoda: Longidoridae) in Costa Rica |
title_short |
Molecular characterization and distribution of the needle nematode Longidorus laevicapitatus Williams, 1959 (Nematoda: Longidoridae) in Costa Rica |
title_full |
Molecular characterization and distribution of the needle nematode Longidorus laevicapitatus Williams, 1959 (Nematoda: Longidoridae) in Costa Rica |
title_fullStr |
Molecular characterization and distribution of the needle nematode Longidorus laevicapitatus Williams, 1959 (Nematoda: Longidoridae) in Costa Rica |
title_full_unstemmed |
Molecular characterization and distribution of the needle nematode Longidorus laevicapitatus Williams, 1959 (Nematoda: Longidoridae) in Costa Rica |
title_sort |
molecular characterization and distribution of the needle nematode longidorus laevicapitatus williams, 1959 (nematoda: longidoridae) in costa rica |
publisher |
Springer |
publishDate |
2017-02 |
url |
http://hdl.handle.net/10261/157199 |
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