Target and Non-target Site Mechanisms Developed by Glyphosate-Resistant Hairy beggarticks (Bidens pilosa L.) Populations from Mexico
In 2014 hairy beggarticks (Bidens pilosa L.) has been identified as being glyphosate-resistant in citrus orchards from Mexico. The target and non-target site mechanisms involved in the response to glyphosate of two resistant populations (R1 and R2) and one susceptible (S) were studied. Experiments of dose-response, shikimic acid accumulation, uptake-translocation, enzyme activity and 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) gene sequencing were carried out in each population. The R1 and R2 populations were 20.4 and 2.8-fold less glyphosate sensitive, respectively, than the S population. The resistant populations showed a lesser shikimic acid accumulation than the S population. In the latter one, 24.9% of 14C-glyphosate was translocated to the roots at 96 h after treatment; in the R1 and R2 populations only 12.9 and 15.5%, respectively, was translocated. Qualitative results confirmed the reduced 14C-glyphosate translocation in the resistant populations. The EPSPS enzyme activity of the S population was 128.4 and 8.5-fold higher than the R1 and R2 populations of glyphosate-treated plants, respectively. A single (Pro-106-Ser), and a double (Thr-102-Ile followed by Pro-106-Ser) mutations were identified in the EPSPS2 gene conferred high resistance in R1 population. Target-site mutations associated with a reduced translocation were responsible for the higher glyphosate resistance in the R1 population. The low-intermediate resistance of the R2 population was mediated by reduced translocation. This is the first glyphosate resistance case confirmed in hairy beggarticks in the world.
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dig-ias-es-10261-1530452021-12-28T16:02:00Z Target and Non-target Site Mechanisms Developed by Glyphosate-Resistant Hairy beggarticks (Bidens pilosa L.) Populations from Mexico Alcántara de la Cruz, Ricardo Fernández-Moreno, Pablo T. Ozuna, Carmen Rojano-Delgado, Antonia María Cruz-Hipólito, Hugo Enrique Domínguez Valenzuela, José A. Barro Losada, Francisco Prado, R. del Comisión Nacional de Investigación Científica y Tecnológica (Chile) In 2014 hairy beggarticks (Bidens pilosa L.) has been identified as being glyphosate-resistant in citrus orchards from Mexico. The target and non-target site mechanisms involved in the response to glyphosate of two resistant populations (R1 and R2) and one susceptible (S) were studied. Experiments of dose-response, shikimic acid accumulation, uptake-translocation, enzyme activity and 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) gene sequencing were carried out in each population. The R1 and R2 populations were 20.4 and 2.8-fold less glyphosate sensitive, respectively, than the S population. The resistant populations showed a lesser shikimic acid accumulation than the S population. In the latter one, 24.9% of 14C-glyphosate was translocated to the roots at 96 h after treatment; in the R1 and R2 populations only 12.9 and 15.5%, respectively, was translocated. Qualitative results confirmed the reduced 14C-glyphosate translocation in the resistant populations. The EPSPS enzyme activity of the S population was 128.4 and 8.5-fold higher than the R1 and R2 populations of glyphosate-treated plants, respectively. A single (Pro-106-Ser), and a double (Thr-102-Ile followed by Pro-106-Ser) mutations were identified in the EPSPS2 gene conferred high resistance in R1 population. Target-site mutations associated with a reduced translocation were responsible for the higher glyphosate resistance in the R1 population. The low-intermediate resistance of the R2 population was mediated by reduced translocation. This is the first glyphosate resistance case confirmed in hairy beggarticks in the world. This work was funded by AGL2013-48946-C3-1-R, and CONACYT-231972 projects. Peer reviewed Peer Reviewed 2017-07-14T12:02:03Z 2017-07-14T12:02:03Z 2016-10-03 2017-07-14T12:02:03Z artículo http://purl.org/coar/resource_type/c_6501 Frontiers in Plant Science 7: 1492 (2016) 1664-462X http://hdl.handle.net/10261/153045 10.3389/fpls.2016.01492 http://dx.doi.org/10.13039/501100002848 27752259 Publisher's version http://dx.doi.org/10.3389/fpls.2016.01492 Sí open Frontiers Media |
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In 2014 hairy beggarticks (Bidens pilosa L.) has been identified as being glyphosate-resistant in citrus orchards from Mexico. The target and non-target site mechanisms involved in the response to glyphosate of two resistant populations (R1 and R2) and one susceptible (S) were studied. Experiments of dose-response, shikimic acid accumulation, uptake-translocation, enzyme activity and 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) gene sequencing were carried out in each population. The R1 and R2 populations were 20.4 and 2.8-fold less glyphosate sensitive, respectively, than the S population. The resistant populations showed a lesser shikimic acid accumulation than the S population. In the latter one, 24.9% of 14C-glyphosate was translocated to the roots at 96 h after treatment; in the R1 and R2 populations only 12.9 and 15.5%, respectively, was translocated. Qualitative results confirmed the reduced 14C-glyphosate translocation in the resistant populations. The EPSPS enzyme activity of the S population was 128.4 and 8.5-fold higher than the R1 and R2 populations of glyphosate-treated plants, respectively. A single (Pro-106-Ser), and a double (Thr-102-Ile followed by Pro-106-Ser) mutations were identified in the EPSPS2 gene conferred high resistance in R1 population. Target-site mutations associated with a reduced translocation were responsible for the higher glyphosate resistance in the R1 population. The low-intermediate resistance of the R2 population was mediated by reduced translocation. This is the first glyphosate resistance case confirmed in hairy beggarticks in the world. |
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Comisión Nacional de Investigación Científica y Tecnológica (Chile) |
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Comisión Nacional de Investigación Científica y Tecnológica (Chile) Alcántara de la Cruz, Ricardo Fernández-Moreno, Pablo T. Ozuna, Carmen Rojano-Delgado, Antonia María Cruz-Hipólito, Hugo Enrique Domínguez Valenzuela, José A. Barro Losada, Francisco Prado, R. del |
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Alcántara de la Cruz, Ricardo Fernández-Moreno, Pablo T. Ozuna, Carmen Rojano-Delgado, Antonia María Cruz-Hipólito, Hugo Enrique Domínguez Valenzuela, José A. Barro Losada, Francisco Prado, R. del |
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Alcántara de la Cruz, Ricardo Fernández-Moreno, Pablo T. Ozuna, Carmen Rojano-Delgado, Antonia María Cruz-Hipólito, Hugo Enrique Domínguez Valenzuela, José A. Barro Losada, Francisco Prado, R. del Target and Non-target Site Mechanisms Developed by Glyphosate-Resistant Hairy beggarticks (Bidens pilosa L.) Populations from Mexico |
author_sort |
Alcántara de la Cruz, Ricardo |
title |
Target and Non-target Site Mechanisms Developed by Glyphosate-Resistant Hairy beggarticks (Bidens pilosa L.) Populations from Mexico |
title_short |
Target and Non-target Site Mechanisms Developed by Glyphosate-Resistant Hairy beggarticks (Bidens pilosa L.) Populations from Mexico |
title_full |
Target and Non-target Site Mechanisms Developed by Glyphosate-Resistant Hairy beggarticks (Bidens pilosa L.) Populations from Mexico |
title_fullStr |
Target and Non-target Site Mechanisms Developed by Glyphosate-Resistant Hairy beggarticks (Bidens pilosa L.) Populations from Mexico |
title_full_unstemmed |
Target and Non-target Site Mechanisms Developed by Glyphosate-Resistant Hairy beggarticks (Bidens pilosa L.) Populations from Mexico |
title_sort |
target and non-target site mechanisms developed by glyphosate-resistant hairy beggarticks (bidens pilosa l.) populations from mexico |
publisher |
Frontiers Media |
publishDate |
2016-10-03 |
url |
http://hdl.handle.net/10261/153045 http://dx.doi.org/10.13039/501100002848 |
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