Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection
Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown. Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large group of genes involved in plant defense. Using high-throughput quantitative real-time PCR (qPCR) technology more than 1000 Medicago truncatula TFs were screened in a pair of susceptible and resistant genotypes of M. truncatula after 4 h of Erysiphe pisi infection. Seventy nine TF genes, belonging to 33 families showed a significant transcriptional change in response to E. pisi infection. Forty eight TF genes were differentially expressed in the resistant genotypes compared to the susceptible one in response to E. pisi infection, including pathogenesis-related transcriptional factors, AP2/EREBP (APETALA2/ETHYLENE-RESPONSIVE ELEMENT BINDING FACTORS), WRKY (highly conserved WRKYGQK amino-acid sequence), MYB (Myeloblastoma), homeodomain (HD) and zinc finger C2C2 (CYS2-CYS2), C2H2, (CYS2-HIS2), LIM (Lin-11, Isl-1, Mec-3) gene families, which are involved in known defense responses. Our results suggest that these TF genes are among the E. pisi responsive genes in resistant M. truncatula that may constitute a regulatory network which controls the transcriptional changes in defense genes involved in resistance to E. pisi.
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2015-07-09
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dig-ias-es-10261-1528722021-12-28T16:45:13Z Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection Curto, Miguel Krajinski, Franziska Schlereth, Armin Rubiales, Diego European Commission Ministerio de Economía y Competitividad (España) Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown. Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large group of genes involved in plant defense. Using high-throughput quantitative real-time PCR (qPCR) technology more than 1000 Medicago truncatula TFs were screened in a pair of susceptible and resistant genotypes of M. truncatula after 4 h of Erysiphe pisi infection. Seventy nine TF genes, belonging to 33 families showed a significant transcriptional change in response to E. pisi infection. Forty eight TF genes were differentially expressed in the resistant genotypes compared to the susceptible one in response to E. pisi infection, including pathogenesis-related transcriptional factors, AP2/EREBP (APETALA2/ETHYLENE-RESPONSIVE ELEMENT BINDING FACTORS), WRKY (highly conserved WRKYGQK amino-acid sequence), MYB (Myeloblastoma), homeodomain (HD) and zinc finger C2C2 (CYS2-CYS2), C2H2, (CYS2-HIS2), LIM (Lin-11, Isl-1, Mec-3) gene families, which are involved in known defense responses. Our results suggest that these TF genes are among the E. pisi responsive genes in resistant M. truncatula that may constitute a regulatory network which controls the transcriptional changes in defense genes involved in resistance to E. pisi. This work was supported by the European Commission FP6 Framework Programme Grain Legume Integrated Project (FOOD-CT-2004-506223) and by Spanish project AGL2014-52871-R co-financed by FEDER. Peer reviewed Peer Reviewed 2017-07-14T11:13:24Z 2017-07-14T11:13:24Z 2015-07-09 2017-07-14T11:13:24Z artículo http://purl.org/coar/resource_type/c_6501 Frontiers in Plant Science 6: 517 (2015) 1664-462X http://hdl.handle.net/10261/152872 10.3389/fpls.2015.00517 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 26217367 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016MINECO/ICTI2013‐2016/AGL2014-52871-R Publisher's version http://dx.doi.org/10.3389/fpls.2015.00517 Sí open Frontiers Media |
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Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown. Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large group of genes involved in plant defense. Using high-throughput quantitative real-time PCR (qPCR) technology more than 1000 Medicago truncatula TFs were screened in a pair of susceptible and resistant genotypes of M. truncatula after 4 h of Erysiphe pisi infection. Seventy nine TF genes, belonging to 33 families showed a significant transcriptional change in response to E. pisi infection. Forty eight TF genes were differentially expressed in the resistant genotypes compared to the susceptible one in response to E. pisi infection, including pathogenesis-related transcriptional factors, AP2/EREBP (APETALA2/ETHYLENE-RESPONSIVE ELEMENT BINDING FACTORS), WRKY (highly conserved WRKYGQK amino-acid sequence), MYB (Myeloblastoma), homeodomain (HD) and zinc finger C2C2 (CYS2-CYS2), C2H2, (CYS2-HIS2), LIM (Lin-11, Isl-1, Mec-3) gene families, which are involved in known defense responses. Our results suggest that these TF genes are among the E. pisi responsive genes in resistant M. truncatula that may constitute a regulatory network which controls the transcriptional changes in defense genes involved in resistance to E. pisi. |
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European Commission |
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European Commission Curto, Miguel Krajinski, Franziska Schlereth, Armin Rubiales, Diego |
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Curto, Miguel Krajinski, Franziska Schlereth, Armin Rubiales, Diego |
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Curto, Miguel Krajinski, Franziska Schlereth, Armin Rubiales, Diego Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection |
author_sort |
Curto, Miguel |
title |
Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection |
title_short |
Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection |
title_full |
Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection |
title_fullStr |
Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection |
title_full_unstemmed |
Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection |
title_sort |
transcriptional profiling of medicago truncatula during erysiphe pisi infection |
publisher |
Frontiers Media |
publishDate |
2015-07-09 |
url |
http://hdl.handle.net/10261/152872 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 |
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1777663103007195136 |