Live attenuated African swine fever viruses as ideal tools to dissect the mechanisms involved in viral pathogenesis and immune protection
Lacasta, Anna et al.
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BioMed Central
2015-11-20
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dig-ias-es-10261-1259422024-10-24T09:44:27Z Live attenuated African swine fever viruses as ideal tools to dissect the mechanisms involved in viral pathogenesis and immune protection Lacasta, Anna Monteagudo, P. L. Jiménez-Marín, Ángeles Accensi, Francesc Ballester, María Argilaguet, Jordi Galindo-Cardiel, I. Segalés, J. Salas Falgueras, María Luisa Domínguez, J. Moreno, Ángela Garrido Pavón, Juan José Rodríguez, Fernando Ministerio de Economía y Competitividad (España) Ministerio de Educación, Cultura y Deporte (España) Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] Lacasta, Anna et al. African swine fever virus (ASFV) is the causal agent of African swine fever, a hemorrhagic and often lethal porcine disease causing enormous economical losses in affected countries. Endemic for decades in most of the sub-Saharan countries and Sardinia, the risk of ASFV-endemicity in Europe has increased since its last introduction into Europe in 2007. Live attenuated viruses have been demonstrated to induce very efficient protective immune responses, albeit most of the time protection was circumscribed to homologous ASFV challenges. However, their use in the field is still far from a reality, mainly due to safety concerns. In this study we compared the course of the in vivo infection caused by two homologous ASFV strains: the virulent E75 and the cell cultured adapted strain E75CV1, obtained from adapting E75 to grow in the CV1 cell-line. Interestingly, the kinetics of both viruses not only differed on the clinical signs that they caused and in the virus loads found, but also in the immunological pathways activated throughout the infections. Furthermore, E75CV1 confirmed its protective potential against the homologous E75 virus challenge and allowed the demonstration of poor cross-protection against BA71, thus defining it as heterologous. The in vitro specificity of the CD8+ T-cells present at the time of lethal challenge showed a clear activation against the homologous virus (E75) but not against BA71. These findings will be of utility for a better understanding of ASFV pathogenesis and for the rational designing of safe and efficient vaccines against this virus. This work has been funded by the Spanish Government (project reference numbers AGL201022229 and AGL201348998C21R). Anna Lacasta and Paula López-Monteagudo were financially supported by an FPU fellowship and an FPI fellowship, respectively, both from the Spanish Government. 2015-11-25T17:58:03Z 2015-11-25T17:58:03Z 2015-11-20 2015-11-25T17:58:03Z artículo http://purl.org/coar/resource_type/c_6501 Veterinary Research 46(1):135 (2015) 0928-4249 http://hdl.handle.net/10261/125942 10.1186/s13567-015-0275-z 1297-9716 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003176 26589145 Centro de Investigación en Sanidad Animal (CISA) Publisher's version http://dx.doi.org/10.1186/s13567-015-0275-z Sí open BioMed Central |
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Lacasta, Anna et al. |
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Ministerio de Economía y Competitividad (España) |
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Ministerio de Economía y Competitividad (España) Lacasta, Anna Monteagudo, P. L. Jiménez-Marín, Ángeles Accensi, Francesc Ballester, María Argilaguet, Jordi Galindo-Cardiel, I. Segalés, J. Salas Falgueras, María Luisa Domínguez, J. Moreno, Ángela Garrido Pavón, Juan José Rodríguez, Fernando |
| format |
artículo |
| author |
Lacasta, Anna Monteagudo, P. L. Jiménez-Marín, Ángeles Accensi, Francesc Ballester, María Argilaguet, Jordi Galindo-Cardiel, I. Segalés, J. Salas Falgueras, María Luisa Domínguez, J. Moreno, Ángela Garrido Pavón, Juan José Rodríguez, Fernando |
| spellingShingle |
Lacasta, Anna Monteagudo, P. L. Jiménez-Marín, Ángeles Accensi, Francesc Ballester, María Argilaguet, Jordi Galindo-Cardiel, I. Segalés, J. Salas Falgueras, María Luisa Domínguez, J. Moreno, Ángela Garrido Pavón, Juan José Rodríguez, Fernando Live attenuated African swine fever viruses as ideal tools to dissect the mechanisms involved in viral pathogenesis and immune protection |
| author_sort |
Lacasta, Anna |
| title |
Live attenuated African swine fever viruses as ideal tools to dissect the mechanisms involved in viral pathogenesis and immune protection |
| title_short |
Live attenuated African swine fever viruses as ideal tools to dissect the mechanisms involved in viral pathogenesis and immune protection |
| title_full |
Live attenuated African swine fever viruses as ideal tools to dissect the mechanisms involved in viral pathogenesis and immune protection |
| title_fullStr |
Live attenuated African swine fever viruses as ideal tools to dissect the mechanisms involved in viral pathogenesis and immune protection |
| title_full_unstemmed |
Live attenuated African swine fever viruses as ideal tools to dissect the mechanisms involved in viral pathogenesis and immune protection |
| title_sort |
live attenuated african swine fever viruses as ideal tools to dissect the mechanisms involved in viral pathogenesis and immune protection |
| publisher |
BioMed Central |
| publishDate |
2015-11-20 |
| url |
http://hdl.handle.net/10261/125942 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003176 |
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