Study of in vivo and in vitro oil palm (Elaeis guineensis Dura x Pisifera) reproductive processes.
Oil palm (Elaeis guneensis Dura x Pisifera) represents a good material for studying somaclonal-variation phenomena as a consequence of in vitro culture. The high genetic instability shown by oil palm plants both in vitro and in vivo is a highly probable source of variations in the regenerated plants and a possible cause of mantled phenotype. To better determine the in vitro reprogramming phenomena and their relation with the floral development, we studied floral genes expression by means of in situ hybridisation experiments with four probes isolated and provided by MPOB (#327 homologous to the Arabidopsis genes RLK5; pOP-SFB107 related to Festuca pratensis Xet1 protein, pOP-SFB6 and pOP-SFB97 connected to the family of Rho-related proteins). The probes were all expressed in situ both in immature inflorescences and in reproductive-like structure obtained from in vitro culture. According to our results there is a stage-specific expression during the in vivo floral development. Moreover, for all the probes so far tested, the clear hybridization signals present in the floral like structures in culture, point out to a deregulated gene expression along the in vitro differentiation processes included somatic embryogenesis.
| Main Authors: | , , , , , , |
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| Format: | Texto biblioteca |
| Language: | ng |
| Published: |
Kuala Lumpur : MPOB,
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| Subjects: | Reproducción., Palma de aceite, |
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| Summary: | Oil palm (Elaeis guneensis Dura x Pisifera) represents a good material for studying somaclonal-variation phenomena as a consequence of in vitro culture. The high genetic instability shown by oil palm plants both in vitro and in vivo is a highly probable source of variations in the regenerated plants and a possible cause of mantled phenotype. To better determine the in vitro reprogramming phenomena and their relation with the floral development, we studied floral genes expression by means of in situ hybridisation experiments with four probes isolated and provided by MPOB (#327 homologous to the Arabidopsis genes RLK5; pOP-SFB107 related to Festuca pratensis Xet1 protein, pOP-SFB6 and pOP-SFB97 connected to the family of Rho-related proteins). The probes were all expressed in situ both in immature inflorescences and in reproductive-like structure obtained from in vitro culture. According to our results there is a stage-specific expression during the in vivo floral development. Moreover, for all the probes so far tested, the clear hybridization signals present in the floral like structures in culture, point out to a deregulated gene expression along the in vitro differentiation processes included somatic embryogenesis. |
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