Fine scale spatial investigation of multiple insecticide resistance and underlying target-site and metabolic mechanisms in Anopheles gambiae in central Côte d'Ivoire
Routine monitoring of occurrence, levels and mechanisms of insecticide resistance informs effective management strategies, and should be used to assess the effect of new tools on resistance. As part of a cluster randomised controlled trial evaluating a novel insecticide-based intervention in central Côte d'Ivoire, we assessed resistance and its underlying mechanisms in Anopheles gambiae populations from a subset of trial villages. Resistance to multiple insecticides in An. gambiae s.s. and An. coluzzii was detected across villages, with dose–response assays demonstrating extremely high resistance intensity to the pyrethroid deltamethrin (> 1,500-fold), and mortality following exposure to pyrethroid-treated bednets was low (< 30% mortality in cone bioassays). The 1014F kdr mutation was almost fixed (≥ 90%) in all villages but the 1575Y kdr-amplifying mutation was relatively rare (< 15%). The carbamate and organophosphate resistance-associated Ace-1 G119S mutation was also detected at moderate frequencies (22–43%). Transcriptome analysis identified overexpression of P450 genes known to confer pyrethroid resistance (Cyp9K1, Cyp6P3, and Cyp6M2), and also a carboxylesterase (COEAE1F) as major candidates. Cyp6P3 expression was high but variable (up to 33-fold) and correlated positively with deltamethrin resistance intensity across villages (r2 = 0.78, P = 0.02). Tools and strategies to mitigate the extreme and multiple resistance provided by these mechanisms are required in this area to avoid future control failures.
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Language: | eng |
Subjects: | Anopheles gambiae, malaria, résistance aux insecticides, résistance aux pesticides, mécanisme de défense, gène, insecticide, deltaméthrine, http://aims.fao.org/aos/agrovoc/c_30508, http://aims.fao.org/aos/agrovoc/c_34312, http://aims.fao.org/aos/agrovoc/c_11794, http://aims.fao.org/aos/agrovoc/c_25427, http://aims.fao.org/aos/agrovoc/c_35269, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_3887, http://aims.fao.org/aos/agrovoc/c_33971, http://aims.fao.org/aos/agrovoc/c_4027, |
Online Access: | http://agritrop.cirad.fr/609725/ http://agritrop.cirad.fr/609725/1/41598_2020_Article_71933.pdf |
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dig-cirad-fr-6097252024-06-25T04:32:25Z http://agritrop.cirad.fr/609725/ http://agritrop.cirad.fr/609725/ Fine scale spatial investigation of multiple insecticide resistance and underlying target-site and metabolic mechanisms in Anopheles gambiae in central Côte d'Ivoire. Oumbouke Welbeck A., Pignatelli Patricia, Barreaux Antoine, Tia Innocent Zran, Koffi Alphonsine A., Ahoua Alou Ludovic P., Sternberg Eleanore D., Thomas Matthew B., Weetman David, N'Guessan Raphaël. 2020. Scientific Reports, 10:15066, 13 phttps://doi.org/10.1038/s41598-020-71933-8 <https://doi.org/10.1038/s41598-020-71933-8> Fine scale spatial investigation of multiple insecticide resistance and underlying target-site and metabolic mechanisms in Anopheles gambiae in central Côte d'Ivoire Oumbouke, Welbeck A. Pignatelli, Patricia Barreaux, Antoine Tia, Innocent Zran Koffi, Alphonsine A. Ahoua Alou, Ludovic P. Sternberg, Eleanore D. Thomas, Matthew B. Weetman, David N'Guessan, Raphaël eng 2020 Scientific Reports Anopheles gambiae malaria résistance aux insecticides résistance aux pesticides mécanisme de défense gène insecticide deltaméthrine http://aims.fao.org/aos/agrovoc/c_30508 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_11794 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3887 http://aims.fao.org/aos/agrovoc/c_33971 Côte d'Ivoire http://aims.fao.org/aos/agrovoc/c_4027 Routine monitoring of occurrence, levels and mechanisms of insecticide resistance informs effective management strategies, and should be used to assess the effect of new tools on resistance. As part of a cluster randomised controlled trial evaluating a novel insecticide-based intervention in central Côte d'Ivoire, we assessed resistance and its underlying mechanisms in Anopheles gambiae populations from a subset of trial villages. Resistance to multiple insecticides in An. gambiae s.s. and An. coluzzii was detected across villages, with dose–response assays demonstrating extremely high resistance intensity to the pyrethroid deltamethrin (> 1,500-fold), and mortality following exposure to pyrethroid-treated bednets was low (< 30% mortality in cone bioassays). The 1014F kdr mutation was almost fixed (≥ 90%) in all villages but the 1575Y kdr-amplifying mutation was relatively rare (< 15%). The carbamate and organophosphate resistance-associated Ace-1 G119S mutation was also detected at moderate frequencies (22–43%). Transcriptome analysis identified overexpression of P450 genes known to confer pyrethroid resistance (Cyp9K1, Cyp6P3, and Cyp6M2), and also a carboxylesterase (COEAE1F) as major candidates. Cyp6P3 expression was high but variable (up to 33-fold) and correlated positively with deltamethrin resistance intensity across villages (r2 = 0.78, P = 0.02). Tools and strategies to mitigate the extreme and multiple resistance provided by these mechanisms are required in this area to avoid future control failures. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/609725/1/41598_2020_Article_71933.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.1038/s41598-020-71933-8 10.1038/s41598-020-71933-8 info:eu-repo/semantics/altIdentifier/doi/10.1038/s41598-020-71933-8 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1038/s41598-020-71933-8 |
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Anopheles gambiae malaria résistance aux insecticides résistance aux pesticides mécanisme de défense gène insecticide deltaméthrine http://aims.fao.org/aos/agrovoc/c_30508 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_11794 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3887 http://aims.fao.org/aos/agrovoc/c_33971 http://aims.fao.org/aos/agrovoc/c_4027 Anopheles gambiae malaria résistance aux insecticides résistance aux pesticides mécanisme de défense gène insecticide deltaméthrine http://aims.fao.org/aos/agrovoc/c_30508 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_11794 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3887 http://aims.fao.org/aos/agrovoc/c_33971 http://aims.fao.org/aos/agrovoc/c_4027 |
spellingShingle |
Anopheles gambiae malaria résistance aux insecticides résistance aux pesticides mécanisme de défense gène insecticide deltaméthrine http://aims.fao.org/aos/agrovoc/c_30508 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_11794 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3887 http://aims.fao.org/aos/agrovoc/c_33971 http://aims.fao.org/aos/agrovoc/c_4027 Anopheles gambiae malaria résistance aux insecticides résistance aux pesticides mécanisme de défense gène insecticide deltaméthrine http://aims.fao.org/aos/agrovoc/c_30508 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_11794 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3887 http://aims.fao.org/aos/agrovoc/c_33971 http://aims.fao.org/aos/agrovoc/c_4027 Oumbouke, Welbeck A. Pignatelli, Patricia Barreaux, Antoine Tia, Innocent Zran Koffi, Alphonsine A. Ahoua Alou, Ludovic P. Sternberg, Eleanore D. Thomas, Matthew B. Weetman, David N'Guessan, Raphaël Fine scale spatial investigation of multiple insecticide resistance and underlying target-site and metabolic mechanisms in Anopheles gambiae in central Côte d'Ivoire |
description |
Routine monitoring of occurrence, levels and mechanisms of insecticide resistance informs effective management strategies, and should be used to assess the effect of new tools on resistance. As part of a cluster randomised controlled trial evaluating a novel insecticide-based intervention in central Côte d'Ivoire, we assessed resistance and its underlying mechanisms in Anopheles gambiae populations from a subset of trial villages. Resistance to multiple insecticides in An. gambiae s.s. and An. coluzzii was detected across villages, with dose–response assays demonstrating extremely high resistance intensity to the pyrethroid deltamethrin (> 1,500-fold), and mortality following exposure to pyrethroid-treated bednets was low (< 30% mortality in cone bioassays). The 1014F kdr mutation was almost fixed (≥ 90%) in all villages but the 1575Y kdr-amplifying mutation was relatively rare (< 15%). The carbamate and organophosphate resistance-associated Ace-1 G119S mutation was also detected at moderate frequencies (22–43%). Transcriptome analysis identified overexpression of P450 genes known to confer pyrethroid resistance (Cyp9K1, Cyp6P3, and Cyp6M2), and also a carboxylesterase (COEAE1F) as major candidates. Cyp6P3 expression was high but variable (up to 33-fold) and correlated positively with deltamethrin resistance intensity across villages (r2 = 0.78, P = 0.02). Tools and strategies to mitigate the extreme and multiple resistance provided by these mechanisms are required in this area to avoid future control failures. |
format |
article |
topic_facet |
Anopheles gambiae malaria résistance aux insecticides résistance aux pesticides mécanisme de défense gène insecticide deltaméthrine http://aims.fao.org/aos/agrovoc/c_30508 http://aims.fao.org/aos/agrovoc/c_34312 http://aims.fao.org/aos/agrovoc/c_11794 http://aims.fao.org/aos/agrovoc/c_25427 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_3887 http://aims.fao.org/aos/agrovoc/c_33971 http://aims.fao.org/aos/agrovoc/c_4027 |
author |
Oumbouke, Welbeck A. Pignatelli, Patricia Barreaux, Antoine Tia, Innocent Zran Koffi, Alphonsine A. Ahoua Alou, Ludovic P. Sternberg, Eleanore D. Thomas, Matthew B. Weetman, David N'Guessan, Raphaël |
author_facet |
Oumbouke, Welbeck A. Pignatelli, Patricia Barreaux, Antoine Tia, Innocent Zran Koffi, Alphonsine A. Ahoua Alou, Ludovic P. Sternberg, Eleanore D. Thomas, Matthew B. Weetman, David N'Guessan, Raphaël |
author_sort |
Oumbouke, Welbeck A. |
title |
Fine scale spatial investigation of multiple insecticide resistance and underlying target-site and metabolic mechanisms in Anopheles gambiae in central Côte d'Ivoire |
title_short |
Fine scale spatial investigation of multiple insecticide resistance and underlying target-site and metabolic mechanisms in Anopheles gambiae in central Côte d'Ivoire |
title_full |
Fine scale spatial investigation of multiple insecticide resistance and underlying target-site and metabolic mechanisms in Anopheles gambiae in central Côte d'Ivoire |
title_fullStr |
Fine scale spatial investigation of multiple insecticide resistance and underlying target-site and metabolic mechanisms in Anopheles gambiae in central Côte d'Ivoire |
title_full_unstemmed |
Fine scale spatial investigation of multiple insecticide resistance and underlying target-site and metabolic mechanisms in Anopheles gambiae in central Côte d'Ivoire |
title_sort |
fine scale spatial investigation of multiple insecticide resistance and underlying target-site and metabolic mechanisms in anopheles gambiae in central côte d'ivoire |
url |
http://agritrop.cirad.fr/609725/ http://agritrop.cirad.fr/609725/1/41598_2020_Article_71933.pdf |
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