Seroprevalence and renal carriage of pathogenic Leptospira in livestock in Cotonou, Benin
Background: Leptospirosis is a zoonotic disease. It is particularly prevalent in tropical countries and has major consequences for human and animal health. In Benin, the disease's epidemiology remains poorly understood, especially in livestock, for which data are lacking. Objectives: To characterise Leptospira seroprevalence and locally circulating serogroups in livestock from Cotonou and to estimate the prevalence of Leptospira renal carriage in cattle. Methods: We conducted a cross-sectional study in February 2020 during which livestock were sampled at an abattoir and in an impoverished city district. We analysed blood samples from 279 livestock animals (i.e. cattle, sheep, goats and pigs) using the microscopic agglutination test. Additionally, samples of renal tissue from 100 cattle underwent 16s rRNA (rrs) real-time PCR analysis. Results: For the 131 cattle, 85 sheep, and 50 goats tested, seroprevalence was 18% (95% confidence interval [CI] [12%, 26%]), 9% (95% CI [4%, 17%] and 2% (95% CI [0%, 9%]), respectively, and most of the seropositive animals were associated with 1:100 titres. All 13 pigs were seronegative. Leptospira DNA was found in the renal tissue of 10% (95% CI [5%, 18%]) of the cattle tested (n = 100). Leptospira borgpetersenii was the main species present (n = 7), but Leptospira interrogans (n = 2) and Leptospira kirschneri (n = 1) were also detected. Various serogroups (Canicola, Grippotyphosa, Sejroe, Icterohaemorrhagiae, Pomona, Pyrogenes, Australis and Autumnalis) were detected using microscopic agglutination test without a clear predominance of any of them. Conclusions: These results suggest that abattoir workers and people living in close contact with livestock in poor urban areas are exposed to the risk of Leptospira infection.
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dig-cirad-fr-6090042024-05-03T07:11:22Z http://agritrop.cirad.fr/609004/ http://agritrop.cirad.fr/609004/ Seroprevalence and renal carriage of pathogenic Leptospira in livestock in Cotonou, Benin. Her Rebecca, Crespin Laurent, Etougbétché Jonas, Groud Karine, Gnolonfoun Mathias, Chapron Audrey, Evenamia Gualbert, Houéménou Gualbert, Lurier Thibaut, Cappelle Julien, Dobigny Gauthier, Ayral Florence. 2024. Veterinary Medicine and Science, 10 (3):e1430, 11 p.https://doi.org/10.1002/vms3.1430 <https://doi.org/10.1002/vms3.1430> Seroprevalence and renal carriage of pathogenic Leptospira in livestock in Cotonou, Benin Her, Rebecca Crespin, Laurent Etougbétché, Jonas Groud, Karine Gnolonfoun, Mathias Chapron, Audrey Evenamia, Gualbert Houéménou, Gualbert Lurier, Thibaut Cappelle, Julien Dobigny, Gauthier Ayral, Florence eng 2024 Veterinary Medicine and Science L73 - Maladies des animaux leptospirose épidémiologie surveillance épidémiologique Leptospira transmission des maladies zoonose morbidité santé animale bétail porcin caprin maladie de l'homme Enquête pathologique ovin bovin http://aims.fao.org/aos/agrovoc/c_4281 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_4280 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_4936 http://aims.fao.org/aos/agrovoc/c_431 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_7555 http://aims.fao.org/aos/agrovoc/c_3324 http://aims.fao.org/aos/agrovoc/c_29198 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_7030 http://aims.fao.org/aos/agrovoc/c_1391 Bénin http://aims.fao.org/aos/agrovoc/c_875 Background: Leptospirosis is a zoonotic disease. It is particularly prevalent in tropical countries and has major consequences for human and animal health. In Benin, the disease's epidemiology remains poorly understood, especially in livestock, for which data are lacking. Objectives: To characterise Leptospira seroprevalence and locally circulating serogroups in livestock from Cotonou and to estimate the prevalence of Leptospira renal carriage in cattle. Methods: We conducted a cross-sectional study in February 2020 during which livestock were sampled at an abattoir and in an impoverished city district. We analysed blood samples from 279 livestock animals (i.e. cattle, sheep, goats and pigs) using the microscopic agglutination test. Additionally, samples of renal tissue from 100 cattle underwent 16s rRNA (rrs) real-time PCR analysis. Results: For the 131 cattle, 85 sheep, and 50 goats tested, seroprevalence was 18% (95% confidence interval [CI] [12%, 26%]), 9% (95% CI [4%, 17%] and 2% (95% CI [0%, 9%]), respectively, and most of the seropositive animals were associated with 1:100 titres. All 13 pigs were seronegative. Leptospira DNA was found in the renal tissue of 10% (95% CI [5%, 18%]) of the cattle tested (n = 100). Leptospira borgpetersenii was the main species present (n = 7), but Leptospira interrogans (n = 2) and Leptospira kirschneri (n = 1) were also detected. Various serogroups (Canicola, Grippotyphosa, Sejroe, Icterohaemorrhagiae, Pomona, Pyrogenes, Australis and Autumnalis) were detected using microscopic agglutination test without a clear predominance of any of them. Conclusions: These results suggest that abattoir workers and people living in close contact with livestock in poor urban areas are exposed to the risk of Leptospira infection. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/609004/1/Veterinary%20Medicine%20Sci%20-%202024%20-%20Her%20-%20Seroprevalence%20and%20renal%20carriage%20of%20pathogenic%20Leptospira%20in%20livestock%20in%20Cotonou.pdf text cc_by_nc_nd info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ https://doi.org/10.1002/vms3.1430 10.1002/vms3.1430 info:eu-repo/semantics/altIdentifier/doi/10.1002/vms3.1430 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1002/vms3.1430 |
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L73 - Maladies des animaux leptospirose épidémiologie surveillance épidémiologique Leptospira transmission des maladies zoonose morbidité santé animale bétail porcin caprin maladie de l'homme Enquête pathologique ovin bovin http://aims.fao.org/aos/agrovoc/c_4281 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_4280 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_4936 http://aims.fao.org/aos/agrovoc/c_431 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_7555 http://aims.fao.org/aos/agrovoc/c_3324 http://aims.fao.org/aos/agrovoc/c_29198 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_7030 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_875 L73 - Maladies des animaux leptospirose épidémiologie surveillance épidémiologique Leptospira transmission des maladies zoonose morbidité santé animale bétail porcin caprin maladie de l'homme Enquête pathologique ovin bovin http://aims.fao.org/aos/agrovoc/c_4281 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_4280 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_4936 http://aims.fao.org/aos/agrovoc/c_431 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_7555 http://aims.fao.org/aos/agrovoc/c_3324 http://aims.fao.org/aos/agrovoc/c_29198 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_7030 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_875 |
spellingShingle |
L73 - Maladies des animaux leptospirose épidémiologie surveillance épidémiologique Leptospira transmission des maladies zoonose morbidité santé animale bétail porcin caprin maladie de l'homme Enquête pathologique ovin bovin http://aims.fao.org/aos/agrovoc/c_4281 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_4280 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_4936 http://aims.fao.org/aos/agrovoc/c_431 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_7555 http://aims.fao.org/aos/agrovoc/c_3324 http://aims.fao.org/aos/agrovoc/c_29198 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_7030 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_875 L73 - Maladies des animaux leptospirose épidémiologie surveillance épidémiologique Leptospira transmission des maladies zoonose morbidité santé animale bétail porcin caprin maladie de l'homme Enquête pathologique ovin bovin http://aims.fao.org/aos/agrovoc/c_4281 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_4280 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_4936 http://aims.fao.org/aos/agrovoc/c_431 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_7555 http://aims.fao.org/aos/agrovoc/c_3324 http://aims.fao.org/aos/agrovoc/c_29198 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_7030 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_875 Her, Rebecca Crespin, Laurent Etougbétché, Jonas Groud, Karine Gnolonfoun, Mathias Chapron, Audrey Evenamia, Gualbert Houéménou, Gualbert Lurier, Thibaut Cappelle, Julien Dobigny, Gauthier Ayral, Florence Seroprevalence and renal carriage of pathogenic Leptospira in livestock in Cotonou, Benin |
description |
Background: Leptospirosis is a zoonotic disease. It is particularly prevalent in tropical countries and has major consequences for human and animal health. In Benin, the disease's epidemiology remains poorly understood, especially in livestock, for which data are lacking. Objectives: To characterise Leptospira seroprevalence and locally circulating serogroups in livestock from Cotonou and to estimate the prevalence of Leptospira renal carriage in cattle. Methods: We conducted a cross-sectional study in February 2020 during which livestock were sampled at an abattoir and in an impoverished city district. We analysed blood samples from 279 livestock animals (i.e. cattle, sheep, goats and pigs) using the microscopic agglutination test. Additionally, samples of renal tissue from 100 cattle underwent 16s rRNA (rrs) real-time PCR analysis. Results: For the 131 cattle, 85 sheep, and 50 goats tested, seroprevalence was 18% (95% confidence interval [CI] [12%, 26%]), 9% (95% CI [4%, 17%] and 2% (95% CI [0%, 9%]), respectively, and most of the seropositive animals were associated with 1:100 titres. All 13 pigs were seronegative. Leptospira DNA was found in the renal tissue of 10% (95% CI [5%, 18%]) of the cattle tested (n = 100). Leptospira borgpetersenii was the main species present (n = 7), but Leptospira interrogans (n = 2) and Leptospira kirschneri (n = 1) were also detected. Various serogroups (Canicola, Grippotyphosa, Sejroe, Icterohaemorrhagiae, Pomona, Pyrogenes, Australis and Autumnalis) were detected using microscopic agglutination test without a clear predominance of any of them. Conclusions: These results suggest that abattoir workers and people living in close contact with livestock in poor urban areas are exposed to the risk of Leptospira infection. |
format |
article |
topic_facet |
L73 - Maladies des animaux leptospirose épidémiologie surveillance épidémiologique Leptospira transmission des maladies zoonose morbidité santé animale bétail porcin caprin maladie de l'homme Enquête pathologique ovin bovin http://aims.fao.org/aos/agrovoc/c_4281 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_4280 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_4936 http://aims.fao.org/aos/agrovoc/c_431 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_7555 http://aims.fao.org/aos/agrovoc/c_3324 http://aims.fao.org/aos/agrovoc/c_29198 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_7030 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_875 |
author |
Her, Rebecca Crespin, Laurent Etougbétché, Jonas Groud, Karine Gnolonfoun, Mathias Chapron, Audrey Evenamia, Gualbert Houéménou, Gualbert Lurier, Thibaut Cappelle, Julien Dobigny, Gauthier Ayral, Florence |
author_facet |
Her, Rebecca Crespin, Laurent Etougbétché, Jonas Groud, Karine Gnolonfoun, Mathias Chapron, Audrey Evenamia, Gualbert Houéménou, Gualbert Lurier, Thibaut Cappelle, Julien Dobigny, Gauthier Ayral, Florence |
author_sort |
Her, Rebecca |
title |
Seroprevalence and renal carriage of pathogenic Leptospira in livestock in Cotonou, Benin |
title_short |
Seroprevalence and renal carriage of pathogenic Leptospira in livestock in Cotonou, Benin |
title_full |
Seroprevalence and renal carriage of pathogenic Leptospira in livestock in Cotonou, Benin |
title_fullStr |
Seroprevalence and renal carriage of pathogenic Leptospira in livestock in Cotonou, Benin |
title_full_unstemmed |
Seroprevalence and renal carriage of pathogenic Leptospira in livestock in Cotonou, Benin |
title_sort |
seroprevalence and renal carriage of pathogenic leptospira in livestock in cotonou, benin |
url |
http://agritrop.cirad.fr/609004/ http://agritrop.cirad.fr/609004/1/Veterinary%20Medicine%20Sci%20-%202024%20-%20Her%20-%20Seroprevalence%20and%20renal%20carriage%20of%20pathogenic%20Leptospira%20in%20livestock%20in%20Cotonou.pdf |
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