Rift Valley fever virus modulates apoptosis and immune response during infection of human astrocytes
Rift Valley fever (RVF) is an arboviral disease of zoonotic origin that causes recurrent epidemics in Africa, the Arabic Peninsula, and islands of the South West of the Indian Ocean. RVF occurs mainly in livestock but also affects humans with severe clinical manifestations, including neurological disorders. However, human neuropathogenesis of Rift Valley fever virus (RVFV) is still poorly characterized. To study the interactions between RVFV and the central nervous system (CNS), we focused on RVFV infection of astrocytes, the major glial cells of the CNS that have several supporting roles including immune response regulation. We confirmed the permissiveness of astrocytes to RVFV infection and highlighted a strain-dependent infectivity. We showed that RVFV infection of astrocytes induced cell apoptosis and observed that the RVFV Non-Structural protein NSs, a known virulence factor, potentially delayed apoptosis by sequestrating activated-caspase 3 in the nucleus. Our study also showed that RVFV-infected astrocytes upregulated expression of genes associated with inflammatory and type I interferon responses at the mRNA level, but not at the protein level. This inhibition of immune response is potentially due to a NSs-dependent mechanism of mRNA nuclear export inhibition. Together, these results highlighted the direct impact of RVFV infection on the human CNS through the induction of apoptosis and a possible inhibition of early-onset immune responses that are crucial for the host survival.
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Francia |
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Europa del Oeste |
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Biblioteca del CIRAD Francia |
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S50 - Santé humaine L73 - Maladies des animaux Virus de la fièvre de la vallée du Rift transmission des maladies fièvre de la Vallée du Rift genre humain zoonose réponse immunitaire maladie de l'homme épidémiologie immunité expression des gènes apoptose infection bétail http://aims.fao.org/aos/agrovoc/c_16463 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_b08d44fd http://aims.fao.org/aos/agrovoc/c_4586 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_3799 http://aims.fao.org/aos/agrovoc/c_29198 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_36634 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_4397 S50 - Santé humaine L73 - Maladies des animaux Virus de la fièvre de la vallée du Rift transmission des maladies fièvre de la Vallée du Rift genre humain zoonose réponse immunitaire maladie de l'homme épidémiologie immunité expression des gènes apoptose infection bétail http://aims.fao.org/aos/agrovoc/c_16463 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_b08d44fd http://aims.fao.org/aos/agrovoc/c_4586 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_3799 http://aims.fao.org/aos/agrovoc/c_29198 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_36634 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_4397 |
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S50 - Santé humaine L73 - Maladies des animaux Virus de la fièvre de la vallée du Rift transmission des maladies fièvre de la Vallée du Rift genre humain zoonose réponse immunitaire maladie de l'homme épidémiologie immunité expression des gènes apoptose infection bétail http://aims.fao.org/aos/agrovoc/c_16463 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_b08d44fd http://aims.fao.org/aos/agrovoc/c_4586 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_3799 http://aims.fao.org/aos/agrovoc/c_29198 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_36634 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_4397 S50 - Santé humaine L73 - Maladies des animaux Virus de la fièvre de la vallée du Rift transmission des maladies fièvre de la Vallée du Rift genre humain zoonose réponse immunitaire maladie de l'homme épidémiologie immunité expression des gènes apoptose infection bétail http://aims.fao.org/aos/agrovoc/c_16463 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_b08d44fd http://aims.fao.org/aos/agrovoc/c_4586 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_3799 http://aims.fao.org/aos/agrovoc/c_29198 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_36634 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_4397 Quellec, Jordan Pédarrieu, Aurélie Piro-Megy, Camille Barthelemy, Jonathan Simonin, Yannick Salinas, Sara Cetre-Sossah, Catherine Rift Valley fever virus modulates apoptosis and immune response during infection of human astrocytes |
description |
Rift Valley fever (RVF) is an arboviral disease of zoonotic origin that causes recurrent epidemics in Africa, the Arabic Peninsula, and islands of the South West of the Indian Ocean. RVF occurs mainly in livestock but also affects humans with severe clinical manifestations, including neurological disorders. However, human neuropathogenesis of Rift Valley fever virus (RVFV) is still poorly characterized. To study the interactions between RVFV and the central nervous system (CNS), we focused on RVFV infection of astrocytes, the major glial cells of the CNS that have several supporting roles including immune response regulation. We confirmed the permissiveness of astrocytes to RVFV infection and highlighted a strain-dependent infectivity. We showed that RVFV infection of astrocytes induced cell apoptosis and observed that the RVFV Non-Structural protein NSs, a known virulence factor, potentially delayed apoptosis by sequestrating activated-caspase 3 in the nucleus. Our study also showed that RVFV-infected astrocytes upregulated expression of genes associated with inflammatory and type I interferon responses at the mRNA level, but not at the protein level. This inhibition of immune response is potentially due to a NSs-dependent mechanism of mRNA nuclear export inhibition. Together, these results highlighted the direct impact of RVFV infection on the human CNS through the induction of apoptosis and a possible inhibition of early-onset immune responses that are crucial for the host survival. |
format |
article |
topic_facet |
S50 - Santé humaine L73 - Maladies des animaux Virus de la fièvre de la vallée du Rift transmission des maladies fièvre de la Vallée du Rift genre humain zoonose réponse immunitaire maladie de l'homme épidémiologie immunité expression des gènes apoptose infection bétail http://aims.fao.org/aos/agrovoc/c_16463 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_b08d44fd http://aims.fao.org/aos/agrovoc/c_4586 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_3799 http://aims.fao.org/aos/agrovoc/c_29198 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_36634 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_4397 |
author |
Quellec, Jordan Pédarrieu, Aurélie Piro-Megy, Camille Barthelemy, Jonathan Simonin, Yannick Salinas, Sara Cetre-Sossah, Catherine |
author_facet |
Quellec, Jordan Pédarrieu, Aurélie Piro-Megy, Camille Barthelemy, Jonathan Simonin, Yannick Salinas, Sara Cetre-Sossah, Catherine |
author_sort |
Quellec, Jordan |
title |
Rift Valley fever virus modulates apoptosis and immune response during infection of human astrocytes |
title_short |
Rift Valley fever virus modulates apoptosis and immune response during infection of human astrocytes |
title_full |
Rift Valley fever virus modulates apoptosis and immune response during infection of human astrocytes |
title_fullStr |
Rift Valley fever virus modulates apoptosis and immune response during infection of human astrocytes |
title_full_unstemmed |
Rift Valley fever virus modulates apoptosis and immune response during infection of human astrocytes |
title_sort |
rift valley fever virus modulates apoptosis and immune response during infection of human astrocytes |
url |
http://agritrop.cirad.fr/608541/ http://agritrop.cirad.fr/608541/1/ID608541.pdf |
work_keys_str_mv |
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_version_ |
1794797496691589120 |
spelling |
dig-cirad-fr-6085412024-03-04T13:25:15Z http://agritrop.cirad.fr/608541/ http://agritrop.cirad.fr/608541/ Rift Valley fever virus modulates apoptosis and immune response during infection of human astrocytes. Quellec Jordan, Pédarrieu Aurélie, Piro-Megy Camille, Barthelemy Jonathan, Simonin Yannick, Salinas Sara, Cetre-Sossah Catherine. 2023. Emerging Microbes & Infections, 12 (1), 16 p.https://doi.org/10.1080/22221751.2023.2207672 <https://doi.org/10.1080/22221751.2023.2207672> Rift Valley fever virus modulates apoptosis and immune response during infection of human astrocytes Quellec, Jordan Pédarrieu, Aurélie Piro-Megy, Camille Barthelemy, Jonathan Simonin, Yannick Salinas, Sara Cetre-Sossah, Catherine eng 2023 Emerging Microbes & Infections S50 - Santé humaine L73 - Maladies des animaux Virus de la fièvre de la vallée du Rift transmission des maladies fièvre de la Vallée du Rift genre humain zoonose réponse immunitaire maladie de l'homme épidémiologie immunité expression des gènes apoptose infection bétail http://aims.fao.org/aos/agrovoc/c_16463 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_b08d44fd http://aims.fao.org/aos/agrovoc/c_4586 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_3799 http://aims.fao.org/aos/agrovoc/c_29198 http://aims.fao.org/aos/agrovoc/c_2615 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_36634 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_4397 Rift Valley fever (RVF) is an arboviral disease of zoonotic origin that causes recurrent epidemics in Africa, the Arabic Peninsula, and islands of the South West of the Indian Ocean. RVF occurs mainly in livestock but also affects humans with severe clinical manifestations, including neurological disorders. However, human neuropathogenesis of Rift Valley fever virus (RVFV) is still poorly characterized. To study the interactions between RVFV and the central nervous system (CNS), we focused on RVFV infection of astrocytes, the major glial cells of the CNS that have several supporting roles including immune response regulation. We confirmed the permissiveness of astrocytes to RVFV infection and highlighted a strain-dependent infectivity. We showed that RVFV infection of astrocytes induced cell apoptosis and observed that the RVFV Non-Structural protein NSs, a known virulence factor, potentially delayed apoptosis by sequestrating activated-caspase 3 in the nucleus. Our study also showed that RVFV-infected astrocytes upregulated expression of genes associated with inflammatory and type I interferon responses at the mRNA level, but not at the protein level. This inhibition of immune response is potentially due to a NSs-dependent mechanism of mRNA nuclear export inhibition. Together, these results highlighted the direct impact of RVFV infection on the human CNS through the induction of apoptosis and a possible inhibition of early-onset immune responses that are crucial for the host survival. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/608541/1/ID608541.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1080/22221751.2023.2207672 10.1080/22221751.2023.2207672 info:eu-repo/semantics/altIdentifier/doi/10.1080/22221751.2023.2207672 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1080/22221751.2023.2207672 info:eu-repo/semantics/reference/purl/https://figshare.com/articles/journal_contribution/Rift_Valley_fever_virus_modulates_apoptosis_and_immune_response_during_infection_of_human_astrocytes/23501887 info:eu-repo/grantAgreement/EC//ANR-21-CE15-0041-02//(FRA) Caractéristiques cliniques et mécanismes moléculaires de la neuroinflammation et des dommages neurovasculaires induits par les arbovirus/ArboNeuro info:eu-repo/grantAgreement/ERDF//ANR-21-CE15-0041-02//(FRA) Caractéristiques cliniques et mécanismes moléculaires de la neuroinflammation et des dommages neurovasculaires induits par les arbovirus/ArboNeuro |