Depicting the core transcriptome modulating multiple abiotic stresses responses in sesame (Sesamum indicum L.)
Sesame is a source of a healthy vegetable oil, attracting a growing interest worldwide. Abiotic stresses have devastating effects on sesame yield; hence, studies have been performed to understand sesame molecular responses to abiotic stresses, but the core abiotic stress-responsive genes (CARG) that the plant reuses in response to an array of environmental stresses are unknown. We performed a meta-analysis of 72 RNA-Seq datasets from drought, waterlogging, salt and osmotic stresses and identified 543 genes constantly and differentially expressed in response to all stresses, representing the sesame CARG. Weighted gene co-expression network analysis of the CARG revealed three functional modules controlled by key transcription factors. Except for salt stress, the modules were positively correlated with the abiotic stresses. Network topology of the modules showed several hub genes predicted to play prominent functions. As proof of concept, we generated over-expressing Arabidopsis lines with hub and non-hub genes. Transgenic plants performed better under drought, waterlogging, and osmotic stresses than the wild-type plants but did not tolerate the salt treatment. As expected, the hub gene was significantly more potent than the non-hub gene. Overall, we discovered several novel candidate genes, which will fuel investigations on plant responses to multiple abiotic stresses.
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dig-cirad-fr-6082672024-02-13T05:32:14Z http://agritrop.cirad.fr/608267/ http://agritrop.cirad.fr/608267/ Depicting the core transcriptome modulating multiple abiotic stresses responses in sesame (Sesamum indicum L.). Dossa Komivi, Mmadi Marie Ali, Zhou Rong, Zhang Tianyuan, Su Ruqi, Zhang Yujuan, Wang Linhai, You Jun, Zhang Xiurong. 2019. International Journal of Molecular Sciences, 20 (16), n.spéc. Plant Genomics 2019:3930, 19 p.https://doi.org/10.3390/ijms20163930 <https://doi.org/10.3390/ijms20163930> Depicting the core transcriptome modulating multiple abiotic stresses responses in sesame (Sesamum indicum L.) Dossa, Komivi Mmadi, Marie Ali Zhou, Rong Zhang, Tianyuan Su, Ruqi Zhang, Yujuan Wang, Linhai You, Jun Zhang, Xiurong eng 2019 International Journal of Molecular Sciences expression des gènes Sesamum indicum génie génétique Transcription génique tolérance à la sécheresse changement climatique stress abiotique stress osmotique stress dû à la sécheresse gène tolérance au sel Arabidopsis thaliana résistance à la sécheresse analyse de réseau http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_6992 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_14914 http://aims.fao.org/aos/agrovoc/c_1666 http://aims.fao.org/aos/agrovoc/c_35768 http://aims.fao.org/aos/agrovoc/c_35750 http://aims.fao.org/aos/agrovoc/c_24993 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_24078 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_2392 http://aims.fao.org/aos/agrovoc/c_5144 Chine http://aims.fao.org/aos/agrovoc/c_1556 Sesame is a source of a healthy vegetable oil, attracting a growing interest worldwide. Abiotic stresses have devastating effects on sesame yield; hence, studies have been performed to understand sesame molecular responses to abiotic stresses, but the core abiotic stress-responsive genes (CARG) that the plant reuses in response to an array of environmental stresses are unknown. We performed a meta-analysis of 72 RNA-Seq datasets from drought, waterlogging, salt and osmotic stresses and identified 543 genes constantly and differentially expressed in response to all stresses, representing the sesame CARG. Weighted gene co-expression network analysis of the CARG revealed three functional modules controlled by key transcription factors. Except for salt stress, the modules were positively correlated with the abiotic stresses. Network topology of the modules showed several hub genes predicted to play prominent functions. As proof of concept, we generated over-expressing Arabidopsis lines with hub and non-hub genes. Transgenic plants performed better under drought, waterlogging, and osmotic stresses than the wild-type plants but did not tolerate the salt treatment. As expected, the hub gene was significantly more potent than the non-hub gene. Overall, we discovered several novel candidate genes, which will fuel investigations on plant responses to multiple abiotic stresses. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/608267/1/ijms-20-03930.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.3390/ijms20163930 10.3390/ijms20163930 info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms20163930 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.3390/ijms20163930 |
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expression des gènes Sesamum indicum génie génétique Transcription génique tolérance à la sécheresse changement climatique stress abiotique stress osmotique stress dû à la sécheresse gène tolérance au sel Arabidopsis thaliana résistance à la sécheresse analyse de réseau http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_6992 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_14914 http://aims.fao.org/aos/agrovoc/c_1666 http://aims.fao.org/aos/agrovoc/c_35768 http://aims.fao.org/aos/agrovoc/c_35750 http://aims.fao.org/aos/agrovoc/c_24993 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_24078 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_2392 http://aims.fao.org/aos/agrovoc/c_5144 http://aims.fao.org/aos/agrovoc/c_1556 expression des gènes Sesamum indicum génie génétique Transcription génique tolérance à la sécheresse changement climatique stress abiotique stress osmotique stress dû à la sécheresse gène tolérance au sel Arabidopsis thaliana résistance à la sécheresse analyse de réseau http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_6992 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_14914 http://aims.fao.org/aos/agrovoc/c_1666 http://aims.fao.org/aos/agrovoc/c_35768 http://aims.fao.org/aos/agrovoc/c_35750 http://aims.fao.org/aos/agrovoc/c_24993 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_24078 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_2392 http://aims.fao.org/aos/agrovoc/c_5144 http://aims.fao.org/aos/agrovoc/c_1556 |
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expression des gènes Sesamum indicum génie génétique Transcription génique tolérance à la sécheresse changement climatique stress abiotique stress osmotique stress dû à la sécheresse gène tolérance au sel Arabidopsis thaliana résistance à la sécheresse analyse de réseau http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_6992 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_14914 http://aims.fao.org/aos/agrovoc/c_1666 http://aims.fao.org/aos/agrovoc/c_35768 http://aims.fao.org/aos/agrovoc/c_35750 http://aims.fao.org/aos/agrovoc/c_24993 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_24078 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_2392 http://aims.fao.org/aos/agrovoc/c_5144 http://aims.fao.org/aos/agrovoc/c_1556 expression des gènes Sesamum indicum génie génétique Transcription génique tolérance à la sécheresse changement climatique stress abiotique stress osmotique stress dû à la sécheresse gène tolérance au sel Arabidopsis thaliana résistance à la sécheresse analyse de réseau http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_6992 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_14914 http://aims.fao.org/aos/agrovoc/c_1666 http://aims.fao.org/aos/agrovoc/c_35768 http://aims.fao.org/aos/agrovoc/c_35750 http://aims.fao.org/aos/agrovoc/c_24993 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_24078 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_2392 http://aims.fao.org/aos/agrovoc/c_5144 http://aims.fao.org/aos/agrovoc/c_1556 Dossa, Komivi Mmadi, Marie Ali Zhou, Rong Zhang, Tianyuan Su, Ruqi Zhang, Yujuan Wang, Linhai You, Jun Zhang, Xiurong Depicting the core transcriptome modulating multiple abiotic stresses responses in sesame (Sesamum indicum L.) |
description |
Sesame is a source of a healthy vegetable oil, attracting a growing interest worldwide. Abiotic stresses have devastating effects on sesame yield; hence, studies have been performed to understand sesame molecular responses to abiotic stresses, but the core abiotic stress-responsive genes (CARG) that the plant reuses in response to an array of environmental stresses are unknown. We performed a meta-analysis of 72 RNA-Seq datasets from drought, waterlogging, salt and osmotic stresses and identified 543 genes constantly and differentially expressed in response to all stresses, representing the sesame CARG. Weighted gene co-expression network analysis of the CARG revealed three functional modules controlled by key transcription factors. Except for salt stress, the modules were positively correlated with the abiotic stresses. Network topology of the modules showed several hub genes predicted to play prominent functions. As proof of concept, we generated over-expressing Arabidopsis lines with hub and non-hub genes. Transgenic plants performed better under drought, waterlogging, and osmotic stresses than the wild-type plants but did not tolerate the salt treatment. As expected, the hub gene was significantly more potent than the non-hub gene. Overall, we discovered several novel candidate genes, which will fuel investigations on plant responses to multiple abiotic stresses. |
format |
article |
topic_facet |
expression des gènes Sesamum indicum génie génétique Transcription génique tolérance à la sécheresse changement climatique stress abiotique stress osmotique stress dû à la sécheresse gène tolérance au sel Arabidopsis thaliana résistance à la sécheresse analyse de réseau http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_6992 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_35128 http://aims.fao.org/aos/agrovoc/c_14914 http://aims.fao.org/aos/agrovoc/c_1666 http://aims.fao.org/aos/agrovoc/c_35768 http://aims.fao.org/aos/agrovoc/c_35750 http://aims.fao.org/aos/agrovoc/c_24993 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_24078 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_2392 http://aims.fao.org/aos/agrovoc/c_5144 http://aims.fao.org/aos/agrovoc/c_1556 |
author |
Dossa, Komivi Mmadi, Marie Ali Zhou, Rong Zhang, Tianyuan Su, Ruqi Zhang, Yujuan Wang, Linhai You, Jun Zhang, Xiurong |
author_facet |
Dossa, Komivi Mmadi, Marie Ali Zhou, Rong Zhang, Tianyuan Su, Ruqi Zhang, Yujuan Wang, Linhai You, Jun Zhang, Xiurong |
author_sort |
Dossa, Komivi |
title |
Depicting the core transcriptome modulating multiple abiotic stresses responses in sesame (Sesamum indicum L.) |
title_short |
Depicting the core transcriptome modulating multiple abiotic stresses responses in sesame (Sesamum indicum L.) |
title_full |
Depicting the core transcriptome modulating multiple abiotic stresses responses in sesame (Sesamum indicum L.) |
title_fullStr |
Depicting the core transcriptome modulating multiple abiotic stresses responses in sesame (Sesamum indicum L.) |
title_full_unstemmed |
Depicting the core transcriptome modulating multiple abiotic stresses responses in sesame (Sesamum indicum L.) |
title_sort |
depicting the core transcriptome modulating multiple abiotic stresses responses in sesame (sesamum indicum l.) |
url |
http://agritrop.cirad.fr/608267/ http://agritrop.cirad.fr/608267/1/ijms-20-03930.pdf |
work_keys_str_mv |
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