Characterization of peroxidase and laccase gene families and in silico identification of potential genes involved in upstream steps of lignan formation in sesame
Peroxidases and laccases are oxidative enzymes involved in physiological processes in plants, covering responses to biotic and abiotic stress as well as biosynthesis of health-promoting specialized metabolites. Although they are thought to be involved in the biosynthesis of (+)-pinoresinol, a comprehensive investigation of this class of enzymes has not yet been conducted in the emerging oil crop sesame and no information is available regarding the potential (+)-pinoresinol synthase genes in this crop. In the present study, we conducted a pan-genome-wide identification of peroxidase and laccase genes coupled with transcriptome profiling of diverse sesame varieties. A total of 83 and 48 genes have been identified as coding for sesame peroxidase and laccase genes, respectively. Based on their protein domain and Arabidopsis thaliana genes used as baits, the genes were classified into nine and seven groups of peroxidase and laccase genes, respectively. The expression of the genes was evaluated using dynamic transcriptome sequencing data from six sesame varieties, including one elite cultivar, white vs black seed varieties, and high vs low oil content varieties. Two peroxidase genes (SiPOD52 and SiPOD63) and two laccase genes (SiLAC1 and SiLAC39), well conserved within the sesame pan-genome and exhibiting consistent expression patterns within sesame varieties matching the kinetic of (+)-pinoresinol accumulation in seeds, were identified as potential (+)-pinoresinol synthase genes. Cis-acting elements of the candidate genes revealed their potential involvement in development, hormonal signaling, and response to light and other abiotic triggers. Transcription factor enrichment analysis of promoter regions showed the predominance of MYB binding sequences. The findings from this study pave the way for lignans-oriented engineering of sesame with wide potential applications in food, health and medicinal domains.
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Format: | article biblioteca |
Language: | eng |
Subjects: | expression des gènes, lignane, gène, laccase, physiologie végétale, biosynthèse, péroxydase, Arabidopsis thaliana, identification, Sesamum radiatum, oxydation, phylogénie, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_36421, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_29513, http://aims.fao.org/aos/agrovoc/c_25189, http://aims.fao.org/aos/agrovoc/c_928, http://aims.fao.org/aos/agrovoc/c_13251, http://aims.fao.org/aos/agrovoc/c_33292, http://aims.fao.org/aos/agrovoc/c_3791, http://aims.fao.org/aos/agrovoc/c_33877, http://aims.fao.org/aos/agrovoc/c_5472, http://aims.fao.org/aos/agrovoc/c_13325, |
Online Access: | http://agritrop.cirad.fr/608262/ http://agritrop.cirad.fr/608262/1/life-12-01200-1.pdf |
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dig-cirad-fr-6082622024-02-12T05:32:15Z http://agritrop.cirad.fr/608262/ http://agritrop.cirad.fr/608262/ Characterization of peroxidase and laccase gene families and in silico identification of potential genes involved in upstream steps of lignan formation in sesame. Bovys Zoclanclounon Yedomon Ange, Rostás Michael, Chung Nam-Jin, Mo Youngjun, Karlovsky Petr, Dossa Komivi. 2022. Life, 12 (8), n.spéc. Research Advances in Plant Genomics: 2nd Edition:1200, 18 p.https://doi.org/10.3390/life12081200 <https://doi.org/10.3390/life12081200> Characterization of peroxidase and laccase gene families and in silico identification of potential genes involved in upstream steps of lignan formation in sesame Bovys Zoclanclounon, Yedomon Ange Rostás, Michael Chung, Nam-Jin Mo, Youngjun Karlovsky, Petr Dossa, Komivi eng 2022 Life expression des gènes lignane gène laccase physiologie végétale biosynthèse péroxydase Arabidopsis thaliana identification Sesamum radiatum oxydation phylogénie http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_36421 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_29513 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_13251 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_33877 http://aims.fao.org/aos/agrovoc/c_5472 http://aims.fao.org/aos/agrovoc/c_13325 Peroxidases and laccases are oxidative enzymes involved in physiological processes in plants, covering responses to biotic and abiotic stress as well as biosynthesis of health-promoting specialized metabolites. Although they are thought to be involved in the biosynthesis of (+)-pinoresinol, a comprehensive investigation of this class of enzymes has not yet been conducted in the emerging oil crop sesame and no information is available regarding the potential (+)-pinoresinol synthase genes in this crop. In the present study, we conducted a pan-genome-wide identification of peroxidase and laccase genes coupled with transcriptome profiling of diverse sesame varieties. A total of 83 and 48 genes have been identified as coding for sesame peroxidase and laccase genes, respectively. Based on their protein domain and Arabidopsis thaliana genes used as baits, the genes were classified into nine and seven groups of peroxidase and laccase genes, respectively. The expression of the genes was evaluated using dynamic transcriptome sequencing data from six sesame varieties, including one elite cultivar, white vs black seed varieties, and high vs low oil content varieties. Two peroxidase genes (SiPOD52 and SiPOD63) and two laccase genes (SiLAC1 and SiLAC39), well conserved within the sesame pan-genome and exhibiting consistent expression patterns within sesame varieties matching the kinetic of (+)-pinoresinol accumulation in seeds, were identified as potential (+)-pinoresinol synthase genes. Cis-acting elements of the candidate genes revealed their potential involvement in development, hormonal signaling, and response to light and other abiotic triggers. Transcription factor enrichment analysis of promoter regions showed the predominance of MYB binding sequences. The findings from this study pave the way for lignans-oriented engineering of sesame with wide potential applications in food, health and medicinal domains. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/608262/1/life-12-01200-1.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.3390/life12081200 10.3390/life12081200 info:eu-repo/semantics/altIdentifier/doi/10.3390/life12081200 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.3390/life12081200 info:eu-repo/semantics/dataset/purl/https://www.ncbi.nlm.nih.gov/bioproject/PRJNA186669/ |
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expression des gènes lignane gène laccase physiologie végétale biosynthèse péroxydase Arabidopsis thaliana identification Sesamum radiatum oxydation phylogénie http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_36421 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_29513 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_13251 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_33877 http://aims.fao.org/aos/agrovoc/c_5472 http://aims.fao.org/aos/agrovoc/c_13325 expression des gènes lignane gène laccase physiologie végétale biosynthèse péroxydase Arabidopsis thaliana identification Sesamum radiatum oxydation phylogénie http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_36421 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_29513 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_13251 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_33877 http://aims.fao.org/aos/agrovoc/c_5472 http://aims.fao.org/aos/agrovoc/c_13325 |
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expression des gènes lignane gène laccase physiologie végétale biosynthèse péroxydase Arabidopsis thaliana identification Sesamum radiatum oxydation phylogénie http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_36421 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_29513 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_13251 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_33877 http://aims.fao.org/aos/agrovoc/c_5472 http://aims.fao.org/aos/agrovoc/c_13325 expression des gènes lignane gène laccase physiologie végétale biosynthèse péroxydase Arabidopsis thaliana identification Sesamum radiatum oxydation phylogénie http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_36421 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_29513 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_13251 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_33877 http://aims.fao.org/aos/agrovoc/c_5472 http://aims.fao.org/aos/agrovoc/c_13325 Bovys Zoclanclounon, Yedomon Ange Rostás, Michael Chung, Nam-Jin Mo, Youngjun Karlovsky, Petr Dossa, Komivi Characterization of peroxidase and laccase gene families and in silico identification of potential genes involved in upstream steps of lignan formation in sesame |
description |
Peroxidases and laccases are oxidative enzymes involved in physiological processes in plants, covering responses to biotic and abiotic stress as well as biosynthesis of health-promoting specialized metabolites. Although they are thought to be involved in the biosynthesis of (+)-pinoresinol, a comprehensive investigation of this class of enzymes has not yet been conducted in the emerging oil crop sesame and no information is available regarding the potential (+)-pinoresinol synthase genes in this crop. In the present study, we conducted a pan-genome-wide identification of peroxidase and laccase genes coupled with transcriptome profiling of diverse sesame varieties. A total of 83 and 48 genes have been identified as coding for sesame peroxidase and laccase genes, respectively. Based on their protein domain and Arabidopsis thaliana genes used as baits, the genes were classified into nine and seven groups of peroxidase and laccase genes, respectively. The expression of the genes was evaluated using dynamic transcriptome sequencing data from six sesame varieties, including one elite cultivar, white vs black seed varieties, and high vs low oil content varieties. Two peroxidase genes (SiPOD52 and SiPOD63) and two laccase genes (SiLAC1 and SiLAC39), well conserved within the sesame pan-genome and exhibiting consistent expression patterns within sesame varieties matching the kinetic of (+)-pinoresinol accumulation in seeds, were identified as potential (+)-pinoresinol synthase genes. Cis-acting elements of the candidate genes revealed their potential involvement in development, hormonal signaling, and response to light and other abiotic triggers. Transcription factor enrichment analysis of promoter regions showed the predominance of MYB binding sequences. The findings from this study pave the way for lignans-oriented engineering of sesame with wide potential applications in food, health and medicinal domains. |
format |
article |
topic_facet |
expression des gènes lignane gène laccase physiologie végétale biosynthèse péroxydase Arabidopsis thaliana identification Sesamum radiatum oxydation phylogénie http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_36421 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_29513 http://aims.fao.org/aos/agrovoc/c_25189 http://aims.fao.org/aos/agrovoc/c_928 http://aims.fao.org/aos/agrovoc/c_13251 http://aims.fao.org/aos/agrovoc/c_33292 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_33877 http://aims.fao.org/aos/agrovoc/c_5472 http://aims.fao.org/aos/agrovoc/c_13325 |
author |
Bovys Zoclanclounon, Yedomon Ange Rostás, Michael Chung, Nam-Jin Mo, Youngjun Karlovsky, Petr Dossa, Komivi |
author_facet |
Bovys Zoclanclounon, Yedomon Ange Rostás, Michael Chung, Nam-Jin Mo, Youngjun Karlovsky, Petr Dossa, Komivi |
author_sort |
Bovys Zoclanclounon, Yedomon Ange |
title |
Characterization of peroxidase and laccase gene families and in silico identification of potential genes involved in upstream steps of lignan formation in sesame |
title_short |
Characterization of peroxidase and laccase gene families and in silico identification of potential genes involved in upstream steps of lignan formation in sesame |
title_full |
Characterization of peroxidase and laccase gene families and in silico identification of potential genes involved in upstream steps of lignan formation in sesame |
title_fullStr |
Characterization of peroxidase and laccase gene families and in silico identification of potential genes involved in upstream steps of lignan formation in sesame |
title_full_unstemmed |
Characterization of peroxidase and laccase gene families and in silico identification of potential genes involved in upstream steps of lignan formation in sesame |
title_sort |
characterization of peroxidase and laccase gene families and in silico identification of potential genes involved in upstream steps of lignan formation in sesame |
url |
http://agritrop.cirad.fr/608262/ http://agritrop.cirad.fr/608262/1/life-12-01200-1.pdf |
work_keys_str_mv |
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