Insight into the control of nodule immunity and senescence during Medicago truncatula symbiosis
Medicago (Medicago truncatula) establishes a symbiosis with the rhizobia Sinorhizobium sp, resulting in the formation of nodules where the bacteria fix atmospheric nitrogen. The loss of immunity repression or early senescence activation compromises symbiont survival and leads to the formation of nonfunctional nodules (fix−). Despite many studies exploring an overlap between immunity and senescence responses outside the nodule context, the relationship between these processes in the nodule remains poorly understood. To investigate this phenomenon, we selected and characterized three Medicago mutants developing fix− nodules and showing senescence responses. Analysis of specific defense (PATHOGENESIS-RELATED PROTEIN) or senescence (CYSTEINE PROTEASE) marker expression demonstrated that senescence and immunity seem to be antagonistic in fix− nodules. The growth of senescence mutants on non-sterile (sand/perlite) substrate instead of sterile in vitro conditions decreased nodule senescence and enhanced defense, indicating that environment can affect the immunity/senescence balance. The application of wounding stress on wild-type (WT) fix+ nodules led to the death of intracellular rhizobia and associated with co-stimulation of defense and senescence markers, indicating that in fix+ nodules the relationship between the two processes switches from opposite to synergistic to control symbiont survival during response to the stress. Our data show that the immune response in stressed WT nodules is linked to the repression of DEFECTIVE IN NITROGEN FIXATION 2 (DNF2), Symbiotic CYSTEINE-RICH RECEPTOR-LIKE KINASE (SymCRK), and REGULATOR OF SYMBIOSOME DIFFERENTIATION (RSD), key genes involved in symbiotic immunity suppression. This study provides insight to understand the links between senescence and immunity in Medicago nodules.
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Language: | eng |
Subjects: | H20 - Maladies des plantes, F60 - Physiologie et biochimie végétale, Fixation de l'azote, symbiose, Medicago truncatula, formation de nodosités, bactérie fixatrice de l'azote, formation atmosphérique, gène de fixation de l'azote, http://aims.fao.org/aos/agrovoc/c_5196, http://aims.fao.org/aos/agrovoc/c_7563, http://aims.fao.org/aos/agrovoc/c_26570, http://aims.fao.org/aos/agrovoc/c_6643, http://aims.fao.org/aos/agrovoc/c_27939, http://aims.fao.org/aos/agrovoc/c_692, http://aims.fao.org/aos/agrovoc/c_331014, |
Online Access: | http://agritrop.cirad.fr/608012/ http://agritrop.cirad.fr/608012/1/kiac505.pdf |
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dig-cirad-fr-6080122024-02-02T14:18:35Z http://agritrop.cirad.fr/608012/ http://agritrop.cirad.fr/608012/ Insight into the control of nodule immunity and senescence during Medicago truncatula symbiosis. Berrabah Fathi, Bernal Gautier, Elhosseyn Ait-Salem, El Kassis Cyrille, L'Horset Roxane, Benaceur Farouk, Wen Jiangqi, Mysore Kirankumar, Garmier Marie, Gourion Benjamin, Ratet Pascal, Gruber Véronique. 2023. Plant Physiology, 191 (1) : 729-746.https://doi.org/10.1093/plphys/kiac505 <https://doi.org/10.1093/plphys/kiac505> Insight into the control of nodule immunity and senescence during Medicago truncatula symbiosis Berrabah, Fathi Bernal, Gautier Elhosseyn, Ait-Salem El Kassis, Cyrille L'Horset, Roxane Benaceur, Farouk Wen, Jiangqi Mysore, Kirankumar Garmier, Marie Gourion, Benjamin Ratet, Pascal Gruber, Véronique eng 2023 Plant Physiology H20 - Maladies des plantes F60 - Physiologie et biochimie végétale Fixation de l'azote symbiose Medicago truncatula formation de nodosités bactérie fixatrice de l'azote formation atmosphérique gène de fixation de l'azote http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_26570 http://aims.fao.org/aos/agrovoc/c_6643 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_692 http://aims.fao.org/aos/agrovoc/c_331014 Medicago (Medicago truncatula) establishes a symbiosis with the rhizobia Sinorhizobium sp, resulting in the formation of nodules where the bacteria fix atmospheric nitrogen. The loss of immunity repression or early senescence activation compromises symbiont survival and leads to the formation of nonfunctional nodules (fix−). Despite many studies exploring an overlap between immunity and senescence responses outside the nodule context, the relationship between these processes in the nodule remains poorly understood. To investigate this phenomenon, we selected and characterized three Medicago mutants developing fix− nodules and showing senescence responses. Analysis of specific defense (PATHOGENESIS-RELATED PROTEIN) or senescence (CYSTEINE PROTEASE) marker expression demonstrated that senescence and immunity seem to be antagonistic in fix− nodules. The growth of senescence mutants on non-sterile (sand/perlite) substrate instead of sterile in vitro conditions decreased nodule senescence and enhanced defense, indicating that environment can affect the immunity/senescence balance. The application of wounding stress on wild-type (WT) fix+ nodules led to the death of intracellular rhizobia and associated with co-stimulation of defense and senescence markers, indicating that in fix+ nodules the relationship between the two processes switches from opposite to synergistic to control symbiont survival during response to the stress. Our data show that the immune response in stressed WT nodules is linked to the repression of DEFECTIVE IN NITROGEN FIXATION 2 (DNF2), Symbiotic CYSTEINE-RICH RECEPTOR-LIKE KINASE (SymCRK), and REGULATOR OF SYMBIOSOME DIFFERENTIATION (RSD), key genes involved in symbiotic immunity suppression. This study provides insight to understand the links between senescence and immunity in Medicago nodules. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/608012/1/kiac505.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1093/plphys/kiac505 10.1093/plphys/kiac505 info:eu-repo/semantics/altIdentifier/doi/10.1093/plphys/kiac505 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1093/plphys/kiac505 info:eu-repo/semantics/dataset/purl/https://www.ncbi.nlm.nih.gov/nuccore/657386114 info:eu-repo/semantics/dataset/purl/https://www.ncbi.nlm.nih.gov/nuccore/66253045 info:eu-repo/grantAgreement///ANR-15-CE20-0005//(FRA) Mécanismes contrôlant la transition entre fixation d'azote et sénescence dans les nodosités symbiotiques de légumineuses/STAYPINK info:eu-repo/grantAgreement///ANR-18-IDEX-0001//(FRA) Université de Paris/ |
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H20 - Maladies des plantes F60 - Physiologie et biochimie végétale Fixation de l'azote symbiose Medicago truncatula formation de nodosités bactérie fixatrice de l'azote formation atmosphérique gène de fixation de l'azote http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_26570 http://aims.fao.org/aos/agrovoc/c_6643 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_692 http://aims.fao.org/aos/agrovoc/c_331014 H20 - Maladies des plantes F60 - Physiologie et biochimie végétale Fixation de l'azote symbiose Medicago truncatula formation de nodosités bactérie fixatrice de l'azote formation atmosphérique gène de fixation de l'azote http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_26570 http://aims.fao.org/aos/agrovoc/c_6643 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_692 http://aims.fao.org/aos/agrovoc/c_331014 |
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H20 - Maladies des plantes F60 - Physiologie et biochimie végétale Fixation de l'azote symbiose Medicago truncatula formation de nodosités bactérie fixatrice de l'azote formation atmosphérique gène de fixation de l'azote http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_26570 http://aims.fao.org/aos/agrovoc/c_6643 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_692 http://aims.fao.org/aos/agrovoc/c_331014 H20 - Maladies des plantes F60 - Physiologie et biochimie végétale Fixation de l'azote symbiose Medicago truncatula formation de nodosités bactérie fixatrice de l'azote formation atmosphérique gène de fixation de l'azote http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_26570 http://aims.fao.org/aos/agrovoc/c_6643 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_692 http://aims.fao.org/aos/agrovoc/c_331014 Berrabah, Fathi Bernal, Gautier Elhosseyn, Ait-Salem El Kassis, Cyrille L'Horset, Roxane Benaceur, Farouk Wen, Jiangqi Mysore, Kirankumar Garmier, Marie Gourion, Benjamin Ratet, Pascal Gruber, Véronique Insight into the control of nodule immunity and senescence during Medicago truncatula symbiosis |
description |
Medicago (Medicago truncatula) establishes a symbiosis with the rhizobia Sinorhizobium sp, resulting in the formation of nodules where the bacteria fix atmospheric nitrogen. The loss of immunity repression or early senescence activation compromises symbiont survival and leads to the formation of nonfunctional nodules (fix−). Despite many studies exploring an overlap between immunity and senescence responses outside the nodule context, the relationship between these processes in the nodule remains poorly understood. To investigate this phenomenon, we selected and characterized three Medicago mutants developing fix− nodules and showing senescence responses. Analysis of specific defense (PATHOGENESIS-RELATED PROTEIN) or senescence (CYSTEINE PROTEASE) marker expression demonstrated that senescence and immunity seem to be antagonistic in fix− nodules. The growth of senescence mutants on non-sterile (sand/perlite) substrate instead of sterile in vitro conditions decreased nodule senescence and enhanced defense, indicating that environment can affect the immunity/senescence balance. The application of wounding stress on wild-type (WT) fix+ nodules led to the death of intracellular rhizobia and associated with co-stimulation of defense and senescence markers, indicating that in fix+ nodules the relationship between the two processes switches from opposite to synergistic to control symbiont survival during response to the stress. Our data show that the immune response in stressed WT nodules is linked to the repression of DEFECTIVE IN NITROGEN FIXATION 2 (DNF2), Symbiotic CYSTEINE-RICH RECEPTOR-LIKE KINASE (SymCRK), and REGULATOR OF SYMBIOSOME DIFFERENTIATION (RSD), key genes involved in symbiotic immunity suppression. This study provides insight to understand the links between senescence and immunity in Medicago nodules. |
format |
article |
topic_facet |
H20 - Maladies des plantes F60 - Physiologie et biochimie végétale Fixation de l'azote symbiose Medicago truncatula formation de nodosités bactérie fixatrice de l'azote formation atmosphérique gène de fixation de l'azote http://aims.fao.org/aos/agrovoc/c_5196 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_26570 http://aims.fao.org/aos/agrovoc/c_6643 http://aims.fao.org/aos/agrovoc/c_27939 http://aims.fao.org/aos/agrovoc/c_692 http://aims.fao.org/aos/agrovoc/c_331014 |
author |
Berrabah, Fathi Bernal, Gautier Elhosseyn, Ait-Salem El Kassis, Cyrille L'Horset, Roxane Benaceur, Farouk Wen, Jiangqi Mysore, Kirankumar Garmier, Marie Gourion, Benjamin Ratet, Pascal Gruber, Véronique |
author_facet |
Berrabah, Fathi Bernal, Gautier Elhosseyn, Ait-Salem El Kassis, Cyrille L'Horset, Roxane Benaceur, Farouk Wen, Jiangqi Mysore, Kirankumar Garmier, Marie Gourion, Benjamin Ratet, Pascal Gruber, Véronique |
author_sort |
Berrabah, Fathi |
title |
Insight into the control of nodule immunity and senescence during Medicago truncatula symbiosis |
title_short |
Insight into the control of nodule immunity and senescence during Medicago truncatula symbiosis |
title_full |
Insight into the control of nodule immunity and senescence during Medicago truncatula symbiosis |
title_fullStr |
Insight into the control of nodule immunity and senescence during Medicago truncatula symbiosis |
title_full_unstemmed |
Insight into the control of nodule immunity and senescence during Medicago truncatula symbiosis |
title_sort |
insight into the control of nodule immunity and senescence during medicago truncatula symbiosis |
url |
http://agritrop.cirad.fr/608012/ http://agritrop.cirad.fr/608012/1/kiac505.pdf |
work_keys_str_mv |
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_version_ |
1792500733951606784 |