Exploring the effects of rearing densities on epigenetic modifications in the zebrafish gonads
Rearing density directly impacts fish welfare, which, in turn, affects productivity in aquaculture. Previous studies have indicated that high-density rearing during sexual development in fish can induce stress, resulting in a tendency towards male-biased sex ratios in the populations. In recent years, research has defined the relevance of the interactions between the environment and epigenetics playing a key role in the final phenotype. However, the underlying epigenetic mechanisms of individuals exposed to confinement remain elucidated. By using zebrafish (Danio rerio), the DNA methylation promotor region and the gene expression patterns of six genes, namely dnmt1, cyp19a1a, dmrt1, cyp11c1, hsd17b1, and hsd11b2, involved in the DNA maintenance methylation, reproduction, and stress were assessed. Zebrafish larvae were subjected to two high-density conditions (9 and 66 fish/L) during two periods of overlapping sex differentiation of this species (7 to 18 and 18 to 45 days post-fertilization, dpf). Results showed a significant masculinization in the populations of fish subjected to high densities from 18 to 45 dpf. In adulthood, the dnmt1 gene was differentially hypomethylated in ovaries and its expression was significantly downregulated in the testes of fish exposed to high-density. Further, the cyp19a1a gene showed downregulation of gene expression in the ovaries of fish subjected to elevated density, as previously observed in other studies. We proposed dnmt1 as a potential testicular epimarker and the expression of ovarian cyp19a1a as a potential biomarker for predicting stress originated from high densities during the early stages of development. These findings highlight the importance of rearing densities by long-lasting effects in adulthood conveying cautions for stocking protocols in fish hatcheries.
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Subjects: | M12 - Production de l'aquaculture, L10 - Génétique et amélioration des animaux, expression des gènes, Oreochromis niloticus, adn, aquaculture, sex ratio, méthylation, gonade, physiologie de la reproduction, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_34687, http://aims.fao.org/aos/agrovoc/c_2347, http://aims.fao.org/aos/agrovoc/c_550, http://aims.fao.org/aos/agrovoc/c_7014, http://aims.fao.org/aos/agrovoc/c_24872, http://aims.fao.org/aos/agrovoc/c_34291, http://aims.fao.org/aos/agrovoc/c_13814, |
Online Access: | http://agritrop.cirad.fr/607149/ http://agritrop.cirad.fr/607149/1/Valdivieso%20et%20al%20IJMS.pdf |
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dig-cirad-fr-6071492024-02-14T08:56:32Z http://agritrop.cirad.fr/607149/ http://agritrop.cirad.fr/607149/ Exploring the effects of rearing densities on epigenetic modifications in the zebrafish gonads. Valdivieso Alejandro, Caballero Huertas Marta, Moraleda-Prados Javier, Piferrer Francesc, Ribas Laia. 2023. International Journal of Molecular Sciences, 24 (21):16002, 18 p.https://doi.org/10.3390/ijms242116002 <https://doi.org/10.3390/ijms242116002> Exploring the effects of rearing densities on epigenetic modifications in the zebrafish gonads Valdivieso, Alejandro Caballero Huertas, Marta Moraleda-Prados, Javier Piferrer, Francesc Ribas, Laia eng 2023 International Journal of Molecular Sciences M12 - Production de l'aquaculture L10 - Génétique et amélioration des animaux expression des gènes Oreochromis niloticus adn aquaculture sex ratio méthylation gonade physiologie de la reproduction http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_34687 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_550 http://aims.fao.org/aos/agrovoc/c_7014 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_34291 http://aims.fao.org/aos/agrovoc/c_13814 Rearing density directly impacts fish welfare, which, in turn, affects productivity in aquaculture. Previous studies have indicated that high-density rearing during sexual development in fish can induce stress, resulting in a tendency towards male-biased sex ratios in the populations. In recent years, research has defined the relevance of the interactions between the environment and epigenetics playing a key role in the final phenotype. However, the underlying epigenetic mechanisms of individuals exposed to confinement remain elucidated. By using zebrafish (Danio rerio), the DNA methylation promotor region and the gene expression patterns of six genes, namely dnmt1, cyp19a1a, dmrt1, cyp11c1, hsd17b1, and hsd11b2, involved in the DNA maintenance methylation, reproduction, and stress were assessed. Zebrafish larvae were subjected to two high-density conditions (9 and 66 fish/L) during two periods of overlapping sex differentiation of this species (7 to 18 and 18 to 45 days post-fertilization, dpf). Results showed a significant masculinization in the populations of fish subjected to high densities from 18 to 45 dpf. In adulthood, the dnmt1 gene was differentially hypomethylated in ovaries and its expression was significantly downregulated in the testes of fish exposed to high-density. Further, the cyp19a1a gene showed downregulation of gene expression in the ovaries of fish subjected to elevated density, as previously observed in other studies. We proposed dnmt1 as a potential testicular epimarker and the expression of ovarian cyp19a1a as a potential biomarker for predicting stress originated from high densities during the early stages of development. These findings highlight the importance of rearing densities by long-lasting effects in adulthood conveying cautions for stocking protocols in fish hatcheries. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/607149/1/Valdivieso%20et%20al%20IJMS.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.3390/ijms242116002 10.3390/ijms242116002 info:eu-repo/semantics/altIdentifier/doi/10.3390/ijms242116002 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.3390/ijms242116002 |
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M12 - Production de l'aquaculture L10 - Génétique et amélioration des animaux expression des gènes Oreochromis niloticus adn aquaculture sex ratio méthylation gonade physiologie de la reproduction http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_34687 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_550 http://aims.fao.org/aos/agrovoc/c_7014 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_34291 http://aims.fao.org/aos/agrovoc/c_13814 M12 - Production de l'aquaculture L10 - Génétique et amélioration des animaux expression des gènes Oreochromis niloticus adn aquaculture sex ratio méthylation gonade physiologie de la reproduction http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_34687 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_550 http://aims.fao.org/aos/agrovoc/c_7014 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_34291 http://aims.fao.org/aos/agrovoc/c_13814 |
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M12 - Production de l'aquaculture L10 - Génétique et amélioration des animaux expression des gènes Oreochromis niloticus adn aquaculture sex ratio méthylation gonade physiologie de la reproduction http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_34687 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_550 http://aims.fao.org/aos/agrovoc/c_7014 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_34291 http://aims.fao.org/aos/agrovoc/c_13814 M12 - Production de l'aquaculture L10 - Génétique et amélioration des animaux expression des gènes Oreochromis niloticus adn aquaculture sex ratio méthylation gonade physiologie de la reproduction http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_34687 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_550 http://aims.fao.org/aos/agrovoc/c_7014 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_34291 http://aims.fao.org/aos/agrovoc/c_13814 Valdivieso, Alejandro Caballero Huertas, Marta Moraleda-Prados, Javier Piferrer, Francesc Ribas, Laia Exploring the effects of rearing densities on epigenetic modifications in the zebrafish gonads |
description |
Rearing density directly impacts fish welfare, which, in turn, affects productivity in aquaculture. Previous studies have indicated that high-density rearing during sexual development in fish can induce stress, resulting in a tendency towards male-biased sex ratios in the populations. In recent years, research has defined the relevance of the interactions between the environment and epigenetics playing a key role in the final phenotype. However, the underlying epigenetic mechanisms of individuals exposed to confinement remain elucidated. By using zebrafish (Danio rerio), the DNA methylation promotor region and the gene expression patterns of six genes, namely dnmt1, cyp19a1a, dmrt1, cyp11c1, hsd17b1, and hsd11b2, involved in the DNA maintenance methylation, reproduction, and stress were assessed. Zebrafish larvae were subjected to two high-density conditions (9 and 66 fish/L) during two periods of overlapping sex differentiation of this species (7 to 18 and 18 to 45 days post-fertilization, dpf). Results showed a significant masculinization in the populations of fish subjected to high densities from 18 to 45 dpf. In adulthood, the dnmt1 gene was differentially hypomethylated in ovaries and its expression was significantly downregulated in the testes of fish exposed to high-density. Further, the cyp19a1a gene showed downregulation of gene expression in the ovaries of fish subjected to elevated density, as previously observed in other studies. We proposed dnmt1 as a potential testicular epimarker and the expression of ovarian cyp19a1a as a potential biomarker for predicting stress originated from high densities during the early stages of development. These findings highlight the importance of rearing densities by long-lasting effects in adulthood conveying cautions for stocking protocols in fish hatcheries. |
format |
article |
topic_facet |
M12 - Production de l'aquaculture L10 - Génétique et amélioration des animaux expression des gènes Oreochromis niloticus adn aquaculture sex ratio méthylation gonade physiologie de la reproduction http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_34687 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_550 http://aims.fao.org/aos/agrovoc/c_7014 http://aims.fao.org/aos/agrovoc/c_24872 http://aims.fao.org/aos/agrovoc/c_34291 http://aims.fao.org/aos/agrovoc/c_13814 |
author |
Valdivieso, Alejandro Caballero Huertas, Marta Moraleda-Prados, Javier Piferrer, Francesc Ribas, Laia |
author_facet |
Valdivieso, Alejandro Caballero Huertas, Marta Moraleda-Prados, Javier Piferrer, Francesc Ribas, Laia |
author_sort |
Valdivieso, Alejandro |
title |
Exploring the effects of rearing densities on epigenetic modifications in the zebrafish gonads |
title_short |
Exploring the effects of rearing densities on epigenetic modifications in the zebrafish gonads |
title_full |
Exploring the effects of rearing densities on epigenetic modifications in the zebrafish gonads |
title_fullStr |
Exploring the effects of rearing densities on epigenetic modifications in the zebrafish gonads |
title_full_unstemmed |
Exploring the effects of rearing densities on epigenetic modifications in the zebrafish gonads |
title_sort |
exploring the effects of rearing densities on epigenetic modifications in the zebrafish gonads |
url |
http://agritrop.cirad.fr/607149/ http://agritrop.cirad.fr/607149/1/Valdivieso%20et%20al%20IJMS.pdf |
work_keys_str_mv |
AT valdiviesoalejandro exploringtheeffectsofrearingdensitiesonepigeneticmodificationsinthezebrafishgonads AT caballerohuertasmarta exploringtheeffectsofrearingdensitiesonepigeneticmodificationsinthezebrafishgonads AT moraledapradosjavier exploringtheeffectsofrearingdensitiesonepigeneticmodificationsinthezebrafishgonads AT piferrerfrancesc exploringtheeffectsofrearingdensitiesonepigeneticmodificationsinthezebrafishgonads AT ribaslaia exploringtheeffectsofrearingdensitiesonepigeneticmodificationsinthezebrafishgonads |
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1792500665912655872 |