Microbiome diversity and composition in Bemisia tabaci SSA1-SG1 whitefly are influenced by their host's life stage
Within the Bemisia tabaci group of cryptic whitefly species, many are damaging agricultural pests and plant-virus vectors, conferring upon this group the status of one of the world's top 100 most invasive and destructive species, affecting farmers' income and threatening their livelihoods. Studies on the microbiome of whitefly life stages are scarce, although their composition and diversity greatly influence whitefly fitness and development. We used high-throughput sequencing to understand microbiome diversity in different developmental stages of the B. tabaci sub-Saharan Africa 1 (SSA1-SG1) species of the whitefly from Uganda. Endosymbionts (Portiera, Arsenophonus, Wolbachia, and Hemipteriphilus were detected but excluded from further statistical analysis as they were not influenced by life stage using Permutational Multivariate Analysis of Variance Using Distance Matrices (ADONIS, p = 0.925 and Bray, p = 0.903). Our results showed significant differences in the meta microbiome composition in different life stages of SSA1-SG1. The diversity was significantly higher in eggs (Shannon, p = 0.024; Simpson, p = 0.047) than that in nymphs and pupae, while the number of microbial species observed by the amplicon sequence variant (ASV) was not significant (n(ASV), p = 0.094). At the phylum and genus levels, the dominant constituents in the microbiome changed significantly during various developmental stages, with Halomonas being present in eggs, whereas Bacillus and Caldalkalibacillus were consistently found across all life stages. These findings provide the first description of differing meta microbiome diversity in the life stage of whiteflies, suggesting their putative role in whitefly development.
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Subjects: | H10 - Ravageurs des plantes, F40 - Écologie végétale, Bemisia tabaci, vecteur de maladie, stade de développement, méthode statistique, http://aims.fao.org/aos/agrovoc/c_30755, http://aims.fao.org/aos/agrovoc/c_8164, http://aims.fao.org/aos/agrovoc/c_2230, http://aims.fao.org/aos/agrovoc/c_7377, http://aims.fao.org/aos/agrovoc/c_8038, http://aims.fao.org/aos/agrovoc/c_166, http://aims.fao.org/aos/agrovoc/c_165, |
Online Access: | http://agritrop.cirad.fr/607023/ http://agritrop.cirad.fr/607023/1/607023.pdf |
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dig-cirad-fr-6070232024-01-29T06:15:55Z http://agritrop.cirad.fr/607023/ http://agritrop.cirad.fr/607023/ Microbiome diversity and composition in Bemisia tabaci SSA1-SG1 whitefly are influenced by their host's life stage. El Hamss Hajar, Maruthi M.N., Omongo Christopher A., Wang Hua-Ling, van Brunschot Sharon, Colvin John, Delatte Hélène. 2023. Microbiological Research, 278:127538, 10 p.https://doi.org/10.1016/j.micres.2023.127538 <https://doi.org/10.1016/j.micres.2023.127538> Microbiome diversity and composition in Bemisia tabaci SSA1-SG1 whitefly are influenced by their host's life stage El Hamss, Hajar Maruthi, M.N. Omongo, Christopher A. Wang, Hua-Ling van Brunschot, Sharon Colvin, John Delatte, Hélène eng 2023 Microbiological Research H10 - Ravageurs des plantes F40 - Écologie végétale Bemisia tabaci vecteur de maladie stade de développement méthode statistique http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2230 http://aims.fao.org/aos/agrovoc/c_7377 Ouganda Afrique au sud du Sahara Afrique http://aims.fao.org/aos/agrovoc/c_8038 http://aims.fao.org/aos/agrovoc/c_166 http://aims.fao.org/aos/agrovoc/c_165 Within the Bemisia tabaci group of cryptic whitefly species, many are damaging agricultural pests and plant-virus vectors, conferring upon this group the status of one of the world's top 100 most invasive and destructive species, affecting farmers' income and threatening their livelihoods. Studies on the microbiome of whitefly life stages are scarce, although their composition and diversity greatly influence whitefly fitness and development. We used high-throughput sequencing to understand microbiome diversity in different developmental stages of the B. tabaci sub-Saharan Africa 1 (SSA1-SG1) species of the whitefly from Uganda. Endosymbionts (Portiera, Arsenophonus, Wolbachia, and Hemipteriphilus were detected but excluded from further statistical analysis as they were not influenced by life stage using Permutational Multivariate Analysis of Variance Using Distance Matrices (ADONIS, p = 0.925 and Bray, p = 0.903). Our results showed significant differences in the meta microbiome composition in different life stages of SSA1-SG1. The diversity was significantly higher in eggs (Shannon, p = 0.024; Simpson, p = 0.047) than that in nymphs and pupae, while the number of microbial species observed by the amplicon sequence variant (ASV) was not significant (n(ASV), p = 0.094). At the phylum and genus levels, the dominant constituents in the microbiome changed significantly during various developmental stages, with Halomonas being present in eggs, whereas Bacillus and Caldalkalibacillus were consistently found across all life stages. These findings provide the first description of differing meta microbiome diversity in the life stage of whiteflies, suggesting their putative role in whitefly development. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/607023/1/607023.pdf text cc_by info:eu-repo/semantics/restrictedAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.1016/j.micres.2023.127538 10.1016/j.micres.2023.127538 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.micres.2023.127538 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.micres.2023.127538 |
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H10 - Ravageurs des plantes F40 - Écologie végétale Bemisia tabaci vecteur de maladie stade de développement méthode statistique http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2230 http://aims.fao.org/aos/agrovoc/c_7377 http://aims.fao.org/aos/agrovoc/c_8038 http://aims.fao.org/aos/agrovoc/c_166 http://aims.fao.org/aos/agrovoc/c_165 H10 - Ravageurs des plantes F40 - Écologie végétale Bemisia tabaci vecteur de maladie stade de développement méthode statistique http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2230 http://aims.fao.org/aos/agrovoc/c_7377 http://aims.fao.org/aos/agrovoc/c_8038 http://aims.fao.org/aos/agrovoc/c_166 http://aims.fao.org/aos/agrovoc/c_165 |
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H10 - Ravageurs des plantes F40 - Écologie végétale Bemisia tabaci vecteur de maladie stade de développement méthode statistique http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2230 http://aims.fao.org/aos/agrovoc/c_7377 http://aims.fao.org/aos/agrovoc/c_8038 http://aims.fao.org/aos/agrovoc/c_166 http://aims.fao.org/aos/agrovoc/c_165 H10 - Ravageurs des plantes F40 - Écologie végétale Bemisia tabaci vecteur de maladie stade de développement méthode statistique http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2230 http://aims.fao.org/aos/agrovoc/c_7377 http://aims.fao.org/aos/agrovoc/c_8038 http://aims.fao.org/aos/agrovoc/c_166 http://aims.fao.org/aos/agrovoc/c_165 El Hamss, Hajar Maruthi, M.N. Omongo, Christopher A. Wang, Hua-Ling van Brunschot, Sharon Colvin, John Delatte, Hélène Microbiome diversity and composition in Bemisia tabaci SSA1-SG1 whitefly are influenced by their host's life stage |
description |
Within the Bemisia tabaci group of cryptic whitefly species, many are damaging agricultural pests and plant-virus vectors, conferring upon this group the status of one of the world's top 100 most invasive and destructive species, affecting farmers' income and threatening their livelihoods. Studies on the microbiome of whitefly life stages are scarce, although their composition and diversity greatly influence whitefly fitness and development. We used high-throughput sequencing to understand microbiome diversity in different developmental stages of the B. tabaci sub-Saharan Africa 1 (SSA1-SG1) species of the whitefly from Uganda. Endosymbionts (Portiera, Arsenophonus, Wolbachia, and Hemipteriphilus were detected but excluded from further statistical analysis as they were not influenced by life stage using Permutational Multivariate Analysis of Variance Using Distance Matrices (ADONIS, p = 0.925 and Bray, p = 0.903). Our results showed significant differences in the meta microbiome composition in different life stages of SSA1-SG1. The diversity was significantly higher in eggs (Shannon, p = 0.024; Simpson, p = 0.047) than that in nymphs and pupae, while the number of microbial species observed by the amplicon sequence variant (ASV) was not significant (n(ASV), p = 0.094). At the phylum and genus levels, the dominant constituents in the microbiome changed significantly during various developmental stages, with Halomonas being present in eggs, whereas Bacillus and Caldalkalibacillus were consistently found across all life stages. These findings provide the first description of differing meta microbiome diversity in the life stage of whiteflies, suggesting their putative role in whitefly development. |
format |
article |
topic_facet |
H10 - Ravageurs des plantes F40 - Écologie végétale Bemisia tabaci vecteur de maladie stade de développement méthode statistique http://aims.fao.org/aos/agrovoc/c_30755 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_2230 http://aims.fao.org/aos/agrovoc/c_7377 http://aims.fao.org/aos/agrovoc/c_8038 http://aims.fao.org/aos/agrovoc/c_166 http://aims.fao.org/aos/agrovoc/c_165 |
author |
El Hamss, Hajar Maruthi, M.N. Omongo, Christopher A. Wang, Hua-Ling van Brunschot, Sharon Colvin, John Delatte, Hélène |
author_facet |
El Hamss, Hajar Maruthi, M.N. Omongo, Christopher A. Wang, Hua-Ling van Brunschot, Sharon Colvin, John Delatte, Hélène |
author_sort |
El Hamss, Hajar |
title |
Microbiome diversity and composition in Bemisia tabaci SSA1-SG1 whitefly are influenced by their host's life stage |
title_short |
Microbiome diversity and composition in Bemisia tabaci SSA1-SG1 whitefly are influenced by their host's life stage |
title_full |
Microbiome diversity and composition in Bemisia tabaci SSA1-SG1 whitefly are influenced by their host's life stage |
title_fullStr |
Microbiome diversity and composition in Bemisia tabaci SSA1-SG1 whitefly are influenced by their host's life stage |
title_full_unstemmed |
Microbiome diversity and composition in Bemisia tabaci SSA1-SG1 whitefly are influenced by their host's life stage |
title_sort |
microbiome diversity and composition in bemisia tabaci ssa1-sg1 whitefly are influenced by their host's life stage |
url |
http://agritrop.cirad.fr/607023/ http://agritrop.cirad.fr/607023/1/607023.pdf |
work_keys_str_mv |
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_version_ |
1792500659672580096 |