Molecular detection of parapoxvirus in Ixodidae ticks collected from cattle in Corsica, France
Background: Several viruses belonging to the family Poxviridae can cause infections in humans and animals. In Corsica, livestock farming (sheep, goats, pigs, and cattle) is mainly mixed, leading to important interactions between livestock, wildlife, and human populations. This could facilitate the circulation of zoonotic diseases, and makes Corsica a good example for studies of tick-borne diseases. Objectives: To gain understanding on the circulation of poxviruses in Corsica, we investigated their presence in tick species collected from cattle, sheep, horses, and wild boar, and characterized them through molecular techniques. Methods: Ticks were tested using specific primers targeting conserved regions of sequences corresponding to two genera: parapoxvirus and orthopoxvirus. Results: A total of 3555 ticks were collected from 1549 different animals (687 cattle, 538 horses, 106 sheep, and 218 wild boars). They were tested for the presence of parapoxvirus DNA on one hand and orthopoxvirus DNA on the other hand using Pangeneric real-time TaqMan assays. Orthopoxvirus DNA was detected in none of the 3555 ticks. Parapoxvirus DNA was detected in 6.6% (36/544) of ticks collected from 23 cows from 20 farms. The remaining 3011 ticks collected from horses, wild boars, and sheep were negative. The infection rate in cow ticks was 8.0% (12/148) in 2018 and 6.0% (24/396) in 2019 (p = 0.57). Parapoxvirus DNA was detected in 8.5% (5/59) of Hyalomma scupense pools, 8.2% (15/183) of Hyalomma marginatum pools, and 6.7% (16/240) of Rhipicephalus bursa pools (p = 0.73). We successfully amplified and sequenced 19.4% (7/36) of the positive samples which all corresponded to pseudocowpox virus. Conclusions: Obviously, further studies are needed to investigate the zoonotic potential of pseudocowpox virus and its importance for animals and public health.
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dig-cirad-fr-6064332024-03-15T14:35:34Z http://agritrop.cirad.fr/606433/ http://agritrop.cirad.fr/606433/ Molecular detection of parapoxvirus in Ixodidae ticks collected from cattle in Corsica, France. Cicculli Vincent, Ayhan Nazli, Luciani Léa, Pezzi Laura, Maitre Apolline, Decarreaux Dorine, De Lamballerie Xavier, Paoli Jean-Christophe, Vial Laurence, Charrel Rémi, Falchi Alessandra. 2022. Veterinary Medicine and Science, 8 (2) : 907-916.https://doi.org/10.1002/vms3.700 <https://doi.org/10.1002/vms3.700> Molecular detection of parapoxvirus in Ixodidae ticks collected from cattle in Corsica, France Cicculli, Vincent Ayhan, Nazli Luciani, Léa Pezzi, Laura Maitre, Apolline Decarreaux, Dorine De Lamballerie, Xavier Paoli, Jean-Christophe Vial, Laurence Charrel, Rémi Falchi, Alessandra eng 2022 Veterinary Medicine and Science L73 - Maladies des animaux L72 - Organismes nuisibles des animaux Ixodidae Parapoxvirus animal sauvage maladie transmissible par tiques bovin transmission des maladies vecteur de maladie zoonose identification ovin bétail taxonomie biologie moléculaire http://aims.fao.org/aos/agrovoc/c_11860 http://aims.fao.org/aos/agrovoc/c_5570 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_7030 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_4891 France Corse http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_1907 Background: Several viruses belonging to the family Poxviridae can cause infections in humans and animals. In Corsica, livestock farming (sheep, goats, pigs, and cattle) is mainly mixed, leading to important interactions between livestock, wildlife, and human populations. This could facilitate the circulation of zoonotic diseases, and makes Corsica a good example for studies of tick-borne diseases. Objectives: To gain understanding on the circulation of poxviruses in Corsica, we investigated their presence in tick species collected from cattle, sheep, horses, and wild boar, and characterized them through molecular techniques. Methods: Ticks were tested using specific primers targeting conserved regions of sequences corresponding to two genera: parapoxvirus and orthopoxvirus. Results: A total of 3555 ticks were collected from 1549 different animals (687 cattle, 538 horses, 106 sheep, and 218 wild boars). They were tested for the presence of parapoxvirus DNA on one hand and orthopoxvirus DNA on the other hand using Pangeneric real-time TaqMan assays. Orthopoxvirus DNA was detected in none of the 3555 ticks. Parapoxvirus DNA was detected in 6.6% (36/544) of ticks collected from 23 cows from 20 farms. The remaining 3011 ticks collected from horses, wild boars, and sheep were negative. The infection rate in cow ticks was 8.0% (12/148) in 2018 and 6.0% (24/396) in 2019 (p = 0.57). Parapoxvirus DNA was detected in 8.5% (5/59) of Hyalomma scupense pools, 8.2% (15/183) of Hyalomma marginatum pools, and 6.7% (16/240) of Rhipicephalus bursa pools (p = 0.73). We successfully amplified and sequenced 19.4% (7/36) of the positive samples which all corresponded to pseudocowpox virus. Conclusions: Obviously, further studies are needed to investigate the zoonotic potential of pseudocowpox virus and its importance for animals and public health. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/606433/1/ID606433.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.1002/vms3.700 10.1002/vms3.700 info:eu-repo/semantics/altIdentifier/doi/10.1002/vms3.700 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1002/vms3.700 |
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Biblioteca del CIRAD Francia |
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L73 - Maladies des animaux L72 - Organismes nuisibles des animaux Ixodidae Parapoxvirus animal sauvage maladie transmissible par tiques bovin transmission des maladies vecteur de maladie zoonose identification ovin bétail taxonomie biologie moléculaire http://aims.fao.org/aos/agrovoc/c_11860 http://aims.fao.org/aos/agrovoc/c_5570 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_7030 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_1907 L73 - Maladies des animaux L72 - Organismes nuisibles des animaux Ixodidae Parapoxvirus animal sauvage maladie transmissible par tiques bovin transmission des maladies vecteur de maladie zoonose identification ovin bétail taxonomie biologie moléculaire http://aims.fao.org/aos/agrovoc/c_11860 http://aims.fao.org/aos/agrovoc/c_5570 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_7030 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_1907 |
spellingShingle |
L73 - Maladies des animaux L72 - Organismes nuisibles des animaux Ixodidae Parapoxvirus animal sauvage maladie transmissible par tiques bovin transmission des maladies vecteur de maladie zoonose identification ovin bétail taxonomie biologie moléculaire http://aims.fao.org/aos/agrovoc/c_11860 http://aims.fao.org/aos/agrovoc/c_5570 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_7030 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_1907 L73 - Maladies des animaux L72 - Organismes nuisibles des animaux Ixodidae Parapoxvirus animal sauvage maladie transmissible par tiques bovin transmission des maladies vecteur de maladie zoonose identification ovin bétail taxonomie biologie moléculaire http://aims.fao.org/aos/agrovoc/c_11860 http://aims.fao.org/aos/agrovoc/c_5570 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_7030 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_1907 Cicculli, Vincent Ayhan, Nazli Luciani, Léa Pezzi, Laura Maitre, Apolline Decarreaux, Dorine De Lamballerie, Xavier Paoli, Jean-Christophe Vial, Laurence Charrel, Rémi Falchi, Alessandra Molecular detection of parapoxvirus in Ixodidae ticks collected from cattle in Corsica, France |
description |
Background: Several viruses belonging to the family Poxviridae can cause infections in humans and animals. In Corsica, livestock farming (sheep, goats, pigs, and cattle) is mainly mixed, leading to important interactions between livestock, wildlife, and human populations. This could facilitate the circulation of zoonotic diseases, and makes Corsica a good example for studies of tick-borne diseases. Objectives: To gain understanding on the circulation of poxviruses in Corsica, we investigated their presence in tick species collected from cattle, sheep, horses, and wild boar, and characterized them through molecular techniques. Methods: Ticks were tested using specific primers targeting conserved regions of sequences corresponding to two genera: parapoxvirus and orthopoxvirus. Results: A total of 3555 ticks were collected from 1549 different animals (687 cattle, 538 horses, 106 sheep, and 218 wild boars). They were tested for the presence of parapoxvirus DNA on one hand and orthopoxvirus DNA on the other hand using Pangeneric real-time TaqMan assays. Orthopoxvirus DNA was detected in none of the 3555 ticks. Parapoxvirus DNA was detected in 6.6% (36/544) of ticks collected from 23 cows from 20 farms. The remaining 3011 ticks collected from horses, wild boars, and sheep were negative. The infection rate in cow ticks was 8.0% (12/148) in 2018 and 6.0% (24/396) in 2019 (p = 0.57). Parapoxvirus DNA was detected in 8.5% (5/59) of Hyalomma scupense pools, 8.2% (15/183) of Hyalomma marginatum pools, and 6.7% (16/240) of Rhipicephalus bursa pools (p = 0.73). We successfully amplified and sequenced 19.4% (7/36) of the positive samples which all corresponded to pseudocowpox virus. Conclusions: Obviously, further studies are needed to investigate the zoonotic potential of pseudocowpox virus and its importance for animals and public health. |
format |
article |
topic_facet |
L73 - Maladies des animaux L72 - Organismes nuisibles des animaux Ixodidae Parapoxvirus animal sauvage maladie transmissible par tiques bovin transmission des maladies vecteur de maladie zoonose identification ovin bétail taxonomie biologie moléculaire http://aims.fao.org/aos/agrovoc/c_11860 http://aims.fao.org/aos/agrovoc/c_5570 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_7030 http://aims.fao.org/aos/agrovoc/c_4397 http://aims.fao.org/aos/agrovoc/c_7631 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_3081 http://aims.fao.org/aos/agrovoc/c_1907 |
author |
Cicculli, Vincent Ayhan, Nazli Luciani, Léa Pezzi, Laura Maitre, Apolline Decarreaux, Dorine De Lamballerie, Xavier Paoli, Jean-Christophe Vial, Laurence Charrel, Rémi Falchi, Alessandra |
author_facet |
Cicculli, Vincent Ayhan, Nazli Luciani, Léa Pezzi, Laura Maitre, Apolline Decarreaux, Dorine De Lamballerie, Xavier Paoli, Jean-Christophe Vial, Laurence Charrel, Rémi Falchi, Alessandra |
author_sort |
Cicculli, Vincent |
title |
Molecular detection of parapoxvirus in Ixodidae ticks collected from cattle in Corsica, France |
title_short |
Molecular detection of parapoxvirus in Ixodidae ticks collected from cattle in Corsica, France |
title_full |
Molecular detection of parapoxvirus in Ixodidae ticks collected from cattle in Corsica, France |
title_fullStr |
Molecular detection of parapoxvirus in Ixodidae ticks collected from cattle in Corsica, France |
title_full_unstemmed |
Molecular detection of parapoxvirus in Ixodidae ticks collected from cattle in Corsica, France |
title_sort |
molecular detection of parapoxvirus in ixodidae ticks collected from cattle in corsica, france |
url |
http://agritrop.cirad.fr/606433/ http://agritrop.cirad.fr/606433/1/ID606433.pdf |
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