Prehaustorial local resistance to coffee leaf rust in a Mexican cultivar involves expression of salicylic acid-responsive genes
Background: In Mexico, coffee leaf rust (CLR) is the main disease that affects the Arabica coffee crop. In this study, the local response of two Mexican cultivars of Coffea arabica (Oro Azteca and Garnica) in the early stages of Hemileia vastatrix infection was evaluated. Methods: We quantified the development of fungal structures in locally-infected leaf disks from both cultivars, using qRT-PCR to measure the relative expression of two pathogenesis recognition genes (CaNDR1 and CaNBS-LRR) and three genes associated with the salicylic acid (SA)-related pathway (CaNPR1, CaPR1, and CaPR5). Results: Resistance of the cv. Oro Azteca was significantly higher than that of the cv. Garnica, with 8.2% and 53.3% haustorial detection, respectively. In addition, the non-race specific disease resistance gene (CaNDR1), a key gene for the pathogen recognition, as well as the genes associated with SA, CaNPR1, CaPR1, and CaPR5, presented an increased expression in response to infection by H. vastatrix in cv. Oro Azteca if comparing with cv. Garnica. Our results suggest that Oro Azteca's defense mechanisms could involve early recognition of CLR by NDR1 and the subsequent activation of the SA signaling pathway.
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dig-cirad-fr-6053632024-01-29T06:02:48Z http://agritrop.cirad.fr/605363/ http://agritrop.cirad.fr/605363/ Prehaustorial local resistance to coffee leaf rust in a Mexican cultivar involves expression of salicylic acid-responsive genes. Couttolenc-Brenis Edgar, Carrion Gloria, Villain Luc, Ortega-Escalona Fernando, Ramírez-Martínez Daniel, Mata-Rosas Martín, Méndez-Bravo Alfonso. 2020. PeerJ, 8:e8345, 21 p.https://doi.org/10.7717/peerj.8345 <https://doi.org/10.7717/peerj.8345> Prehaustorial local resistance to coffee leaf rust in a Mexican cultivar involves expression of salicylic acid-responsive genes Couttolenc-Brenis, Edgar Carrion, Gloria Villain, Luc Ortega-Escalona, Fernando Ramírez-Martínez, Daniel Mata-Rosas, Martín Méndez-Bravo, Alfonso eng 2020 PeerJ Coffea arabica Hemileia vastatrix expression des gènes maladie des plantes rouille résistance aux maladies Coffea canephora variété Azteca mécanisme de défense réaction de défense des plantes Coffea gène http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_32323 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5962 http://aims.fao.org/aos/agrovoc/c_6710 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_1723 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_29892 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_36991 http://aims.fao.org/aos/agrovoc/c_1720 http://aims.fao.org/aos/agrovoc/c_3214 Mexique http://aims.fao.org/aos/agrovoc/c_4792 Background: In Mexico, coffee leaf rust (CLR) is the main disease that affects the Arabica coffee crop. In this study, the local response of two Mexican cultivars of Coffea arabica (Oro Azteca and Garnica) in the early stages of Hemileia vastatrix infection was evaluated. Methods: We quantified the development of fungal structures in locally-infected leaf disks from both cultivars, using qRT-PCR to measure the relative expression of two pathogenesis recognition genes (CaNDR1 and CaNBS-LRR) and three genes associated with the salicylic acid (SA)-related pathway (CaNPR1, CaPR1, and CaPR5). Results: Resistance of the cv. Oro Azteca was significantly higher than that of the cv. Garnica, with 8.2% and 53.3% haustorial detection, respectively. In addition, the non-race specific disease resistance gene (CaNDR1), a key gene for the pathogen recognition, as well as the genes associated with SA, CaNPR1, CaPR1, and CaPR5, presented an increased expression in response to infection by H. vastatrix in cv. Oro Azteca if comparing with cv. Garnica. Our results suggest that Oro Azteca's defense mechanisms could involve early recognition of CLR by NDR1 and the subsequent activation of the SA signaling pathway. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/605363/1/605363.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.7717/peerj.8345 10.7717/peerj.8345 info:eu-repo/semantics/altIdentifier/doi/10.7717/peerj.8345 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.7717/peerj.8345 |
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Biblioteca del CIRAD Francia |
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Coffea arabica Hemileia vastatrix expression des gènes maladie des plantes rouille résistance aux maladies Coffea canephora variété Azteca mécanisme de défense réaction de défense des plantes Coffea gène http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_32323 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5962 http://aims.fao.org/aos/agrovoc/c_6710 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_1723 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_29892 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_36991 http://aims.fao.org/aos/agrovoc/c_1720 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_4792 Coffea arabica Hemileia vastatrix expression des gènes maladie des plantes rouille résistance aux maladies Coffea canephora variété Azteca mécanisme de défense réaction de défense des plantes Coffea gène http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_32323 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5962 http://aims.fao.org/aos/agrovoc/c_6710 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_1723 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_29892 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_36991 http://aims.fao.org/aos/agrovoc/c_1720 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_4792 |
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Coffea arabica Hemileia vastatrix expression des gènes maladie des plantes rouille résistance aux maladies Coffea canephora variété Azteca mécanisme de défense réaction de défense des plantes Coffea gène http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_32323 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5962 http://aims.fao.org/aos/agrovoc/c_6710 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_1723 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_29892 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_36991 http://aims.fao.org/aos/agrovoc/c_1720 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_4792 Coffea arabica Hemileia vastatrix expression des gènes maladie des plantes rouille résistance aux maladies Coffea canephora variété Azteca mécanisme de défense réaction de défense des plantes Coffea gène http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_32323 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5962 http://aims.fao.org/aos/agrovoc/c_6710 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_1723 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_29892 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_36991 http://aims.fao.org/aos/agrovoc/c_1720 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_4792 Couttolenc-Brenis, Edgar Carrion, Gloria Villain, Luc Ortega-Escalona, Fernando Ramírez-Martínez, Daniel Mata-Rosas, Martín Méndez-Bravo, Alfonso Prehaustorial local resistance to coffee leaf rust in a Mexican cultivar involves expression of salicylic acid-responsive genes |
description |
Background: In Mexico, coffee leaf rust (CLR) is the main disease that affects the Arabica coffee crop. In this study, the local response of two Mexican cultivars of Coffea arabica (Oro Azteca and Garnica) in the early stages of Hemileia vastatrix infection was evaluated. Methods: We quantified the development of fungal structures in locally-infected leaf disks from both cultivars, using qRT-PCR to measure the relative expression of two pathogenesis recognition genes (CaNDR1 and CaNBS-LRR) and three genes associated with the salicylic acid (SA)-related pathway (CaNPR1, CaPR1, and CaPR5). Results: Resistance of the cv. Oro Azteca was significantly higher than that of the cv. Garnica, with 8.2% and 53.3% haustorial detection, respectively. In addition, the non-race specific disease resistance gene (CaNDR1), a key gene for the pathogen recognition, as well as the genes associated with SA, CaNPR1, CaPR1, and CaPR5, presented an increased expression in response to infection by H. vastatrix in cv. Oro Azteca if comparing with cv. Garnica. Our results suggest that Oro Azteca's defense mechanisms could involve early recognition of CLR by NDR1 and the subsequent activation of the SA signaling pathway. |
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article |
topic_facet |
Coffea arabica Hemileia vastatrix expression des gènes maladie des plantes rouille résistance aux maladies Coffea canephora variété Azteca mécanisme de défense réaction de défense des plantes Coffea gène http://aims.fao.org/aos/agrovoc/c_1721 http://aims.fao.org/aos/agrovoc/c_32323 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_5962 http://aims.fao.org/aos/agrovoc/c_6710 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_1723 http://aims.fao.org/aos/agrovoc/c_8157 http://aims.fao.org/aos/agrovoc/c_29892 http://aims.fao.org/aos/agrovoc/c_35269 http://aims.fao.org/aos/agrovoc/c_36991 http://aims.fao.org/aos/agrovoc/c_1720 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_4792 |
author |
Couttolenc-Brenis, Edgar Carrion, Gloria Villain, Luc Ortega-Escalona, Fernando Ramírez-Martínez, Daniel Mata-Rosas, Martín Méndez-Bravo, Alfonso |
author_facet |
Couttolenc-Brenis, Edgar Carrion, Gloria Villain, Luc Ortega-Escalona, Fernando Ramírez-Martínez, Daniel Mata-Rosas, Martín Méndez-Bravo, Alfonso |
author_sort |
Couttolenc-Brenis, Edgar |
title |
Prehaustorial local resistance to coffee leaf rust in a Mexican cultivar involves expression of salicylic acid-responsive genes |
title_short |
Prehaustorial local resistance to coffee leaf rust in a Mexican cultivar involves expression of salicylic acid-responsive genes |
title_full |
Prehaustorial local resistance to coffee leaf rust in a Mexican cultivar involves expression of salicylic acid-responsive genes |
title_fullStr |
Prehaustorial local resistance to coffee leaf rust in a Mexican cultivar involves expression of salicylic acid-responsive genes |
title_full_unstemmed |
Prehaustorial local resistance to coffee leaf rust in a Mexican cultivar involves expression of salicylic acid-responsive genes |
title_sort |
prehaustorial local resistance to coffee leaf rust in a mexican cultivar involves expression of salicylic acid-responsive genes |
url |
http://agritrop.cirad.fr/605363/ http://agritrop.cirad.fr/605363/1/605363.pdf |
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