Methyl jasmonate induces oxidative/nitrosative stress and the accumulation of antioxidant metabolites in Phoenix dactylifera L.
Objectives: The present study aimed to explore the eliciting effects of increasing concentrations (50, 100, and 200 µM) of methyl jasmonate (MeJA). We cultivated actively proliferating buds of Phoenix dactylifera L. cv. Barhee in a temporary immersion system and we monitored the bioactive compound accumulation after 7 days of culture. Methods: Total phenolic (TPC) and flavonoid (TFC) contents were determined by high-performance liquid chromatography (HPLC), Fourier-transform infrared (FTIR), and radical scavenging activity using DPPH and ABTS assays. We also explored the activity of phenylpropanoid pathway enzymes, namely phenylalanine ammonia-lyase (PAL), tyrosine ammonia-lyase (TAL) and polyphenol oxidase (PPO). Results: Our results revealed that MeJA treatment induced oxidative stress, and at the same time increased the activity of related defense enzymes in a dose-dependent manner. Exogenous application of MeJA at 200 µM increased ROS (two fold), hydrogen peroxide (3.7 fold), nitric oxide (14 fold), MDA (6.3 fold), superoxide dismutase (5.9 fold), catalase (4.4 fold) and guaiacol peroxidase (3.87 fold). Furthermore, the results demonstrated that 200 µM MeJA treatment enhanced the activities of PAL (3.65 fold), TAL (4.35 fold), PPO (threefold) and increased TPC (twofold) and TFC (1.75 fold) contents in buds cultures higher than the control. HPLC analysis showed that buds cultures exposed to 200 µM MeJA accumulated maximum amount of catechin (11 fold), 4-hydroxybenzoic acid (1.48 fold), caffeic acid (2.5 fold) and p-coumaric acid (1.76 fold) and demonstrate antioxidant capacity with the lowest DPPH (114.5 µg ml−1) and ABTS (90.2 µg ml−1) IC50 values on day 7 of culture as compared to the control. The MeJA in the culture medium directly reduced cell viability in a dose dependent manner up to 35% with the highest concentration. Conclusion: The results of this study has revealed, for the first time, that MeJA offers a promising potential for the production of phenolic compound in Phoenix dactylifera L. buds.
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Subjects: | F60 - Physiologie et biochimie végétale, Q04 - Composition des produits alimentaires, composé phénolique, antioxydant, culture in vitro, composition chimique, activité enzymatique, catéchol oxydase, méthyl jasmonate, stress oxydatif, Phoenix dactylifera, http://aims.fao.org/aos/agrovoc/c_5772, http://aims.fao.org/aos/agrovoc/c_511, http://aims.fao.org/aos/agrovoc/c_24135, http://aims.fao.org/aos/agrovoc/c_1794, http://aims.fao.org/aos/agrovoc/c_2604, http://aims.fao.org/aos/agrovoc/c_29496, http://aims.fao.org/aos/agrovoc/c_36638, http://aims.fao.org/aos/agrovoc/c_37481, http://aims.fao.org/aos/agrovoc/c_5791, |
Online Access: | http://agritrop.cirad.fr/604986/ http://agritrop.cirad.fr/604986/1/4a2ae22c-c578-446b-9ea3-7497e81eda40.PDF |
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dig-cirad-fr-6049862024-03-08T15:03:15Z http://agritrop.cirad.fr/604986/ http://agritrop.cirad.fr/604986/ Methyl jasmonate induces oxidative/nitrosative stress and the accumulation of antioxidant metabolites in Phoenix dactylifera L. Ben Romdhane Walid, Chtourou Yassine, Sebii Haifa, Baklouti Emna, Nasri Ameni, Drira Riadh, Maalej Mohamed, Drira Noureddine, Rival Alain, Fki Lotfi. 2022. Biotechnology Letters, 44 : 1323-1336.https://doi.org/10.1007/s10529-022-03299-y <https://doi.org/10.1007/s10529-022-03299-y> Methyl jasmonate induces oxidative/nitrosative stress and the accumulation of antioxidant metabolites in Phoenix dactylifera L. Ben Romdhane, Walid Chtourou, Yassine Sebii, Haifa Baklouti, Emna Nasri, Ameni Drira, Riadh Maalej, Mohamed Drira, Noureddine Rival, Alain Fki, Lotfi eng 2022 Biotechnology Letters F60 - Physiologie et biochimie végétale Q04 - Composition des produits alimentaires composé phénolique antioxydant culture in vitro composition chimique activité enzymatique catéchol oxydase méthyl jasmonate stress oxydatif Phoenix dactylifera http://aims.fao.org/aos/agrovoc/c_5772 http://aims.fao.org/aos/agrovoc/c_511 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_29496 http://aims.fao.org/aos/agrovoc/c_36638 http://aims.fao.org/aos/agrovoc/c_37481 http://aims.fao.org/aos/agrovoc/c_5791 Objectives: The present study aimed to explore the eliciting effects of increasing concentrations (50, 100, and 200 µM) of methyl jasmonate (MeJA). We cultivated actively proliferating buds of Phoenix dactylifera L. cv. Barhee in a temporary immersion system and we monitored the bioactive compound accumulation after 7 days of culture. Methods: Total phenolic (TPC) and flavonoid (TFC) contents were determined by high-performance liquid chromatography (HPLC), Fourier-transform infrared (FTIR), and radical scavenging activity using DPPH and ABTS assays. We also explored the activity of phenylpropanoid pathway enzymes, namely phenylalanine ammonia-lyase (PAL), tyrosine ammonia-lyase (TAL) and polyphenol oxidase (PPO). Results: Our results revealed that MeJA treatment induced oxidative stress, and at the same time increased the activity of related defense enzymes in a dose-dependent manner. Exogenous application of MeJA at 200 µM increased ROS (two fold), hydrogen peroxide (3.7 fold), nitric oxide (14 fold), MDA (6.3 fold), superoxide dismutase (5.9 fold), catalase (4.4 fold) and guaiacol peroxidase (3.87 fold). Furthermore, the results demonstrated that 200 µM MeJA treatment enhanced the activities of PAL (3.65 fold), TAL (4.35 fold), PPO (threefold) and increased TPC (twofold) and TFC (1.75 fold) contents in buds cultures higher than the control. HPLC analysis showed that buds cultures exposed to 200 µM MeJA accumulated maximum amount of catechin (11 fold), 4-hydroxybenzoic acid (1.48 fold), caffeic acid (2.5 fold) and p-coumaric acid (1.76 fold) and demonstrate antioxidant capacity with the lowest DPPH (114.5 µg ml−1) and ABTS (90.2 µg ml−1) IC50 values on day 7 of culture as compared to the control. The MeJA in the culture medium directly reduced cell viability in a dose dependent manner up to 35% with the highest concentration. Conclusion: The results of this study has revealed, for the first time, that MeJA offers a promising potential for the production of phenolic compound in Phoenix dactylifera L. buds. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/acceptedVersion http://agritrop.cirad.fr/604986/1/4a2ae22c-c578-446b-9ea3-7497e81eda40.PDF other cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.1007/s10529-022-03299-y 10.1007/s10529-022-03299-y info:eu-repo/semantics/altIdentifier/doi/10.1007/s10529-022-03299-y info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s10529-022-03299-y |
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F60 - Physiologie et biochimie végétale Q04 - Composition des produits alimentaires composé phénolique antioxydant culture in vitro composition chimique activité enzymatique catéchol oxydase méthyl jasmonate stress oxydatif Phoenix dactylifera http://aims.fao.org/aos/agrovoc/c_5772 http://aims.fao.org/aos/agrovoc/c_511 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_29496 http://aims.fao.org/aos/agrovoc/c_36638 http://aims.fao.org/aos/agrovoc/c_37481 http://aims.fao.org/aos/agrovoc/c_5791 F60 - Physiologie et biochimie végétale Q04 - Composition des produits alimentaires composé phénolique antioxydant culture in vitro composition chimique activité enzymatique catéchol oxydase méthyl jasmonate stress oxydatif Phoenix dactylifera http://aims.fao.org/aos/agrovoc/c_5772 http://aims.fao.org/aos/agrovoc/c_511 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_29496 http://aims.fao.org/aos/agrovoc/c_36638 http://aims.fao.org/aos/agrovoc/c_37481 http://aims.fao.org/aos/agrovoc/c_5791 |
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F60 - Physiologie et biochimie végétale Q04 - Composition des produits alimentaires composé phénolique antioxydant culture in vitro composition chimique activité enzymatique catéchol oxydase méthyl jasmonate stress oxydatif Phoenix dactylifera http://aims.fao.org/aos/agrovoc/c_5772 http://aims.fao.org/aos/agrovoc/c_511 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_29496 http://aims.fao.org/aos/agrovoc/c_36638 http://aims.fao.org/aos/agrovoc/c_37481 http://aims.fao.org/aos/agrovoc/c_5791 F60 - Physiologie et biochimie végétale Q04 - Composition des produits alimentaires composé phénolique antioxydant culture in vitro composition chimique activité enzymatique catéchol oxydase méthyl jasmonate stress oxydatif Phoenix dactylifera http://aims.fao.org/aos/agrovoc/c_5772 http://aims.fao.org/aos/agrovoc/c_511 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_29496 http://aims.fao.org/aos/agrovoc/c_36638 http://aims.fao.org/aos/agrovoc/c_37481 http://aims.fao.org/aos/agrovoc/c_5791 Ben Romdhane, Walid Chtourou, Yassine Sebii, Haifa Baklouti, Emna Nasri, Ameni Drira, Riadh Maalej, Mohamed Drira, Noureddine Rival, Alain Fki, Lotfi Methyl jasmonate induces oxidative/nitrosative stress and the accumulation of antioxidant metabolites in Phoenix dactylifera L. |
description |
Objectives: The present study aimed to explore the eliciting effects of increasing concentrations (50, 100, and 200 µM) of methyl jasmonate (MeJA). We cultivated actively proliferating buds of Phoenix dactylifera L. cv. Barhee in a temporary immersion system and we monitored the bioactive compound accumulation after 7 days of culture. Methods: Total phenolic (TPC) and flavonoid (TFC) contents were determined by high-performance liquid chromatography (HPLC), Fourier-transform infrared (FTIR), and radical scavenging activity using DPPH and ABTS assays. We also explored the activity of phenylpropanoid pathway enzymes, namely phenylalanine ammonia-lyase (PAL), tyrosine ammonia-lyase (TAL) and polyphenol oxidase (PPO). Results: Our results revealed that MeJA treatment induced oxidative stress, and at the same time increased the activity of related defense enzymes in a dose-dependent manner. Exogenous application of MeJA at 200 µM increased ROS (two fold), hydrogen peroxide (3.7 fold), nitric oxide (14 fold), MDA (6.3 fold), superoxide dismutase (5.9 fold), catalase (4.4 fold) and guaiacol peroxidase (3.87 fold). Furthermore, the results demonstrated that 200 µM MeJA treatment enhanced the activities of PAL (3.65 fold), TAL (4.35 fold), PPO (threefold) and increased TPC (twofold) and TFC (1.75 fold) contents in buds cultures higher than the control. HPLC analysis showed that buds cultures exposed to 200 µM MeJA accumulated maximum amount of catechin (11 fold), 4-hydroxybenzoic acid (1.48 fold), caffeic acid (2.5 fold) and p-coumaric acid (1.76 fold) and demonstrate antioxidant capacity with the lowest DPPH (114.5 µg ml−1) and ABTS (90.2 µg ml−1) IC50 values on day 7 of culture as compared to the control. The MeJA in the culture medium directly reduced cell viability in a dose dependent manner up to 35% with the highest concentration. Conclusion: The results of this study has revealed, for the first time, that MeJA offers a promising potential for the production of phenolic compound in Phoenix dactylifera L. buds. |
format |
article |
topic_facet |
F60 - Physiologie et biochimie végétale Q04 - Composition des produits alimentaires composé phénolique antioxydant culture in vitro composition chimique activité enzymatique catéchol oxydase méthyl jasmonate stress oxydatif Phoenix dactylifera http://aims.fao.org/aos/agrovoc/c_5772 http://aims.fao.org/aos/agrovoc/c_511 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_1794 http://aims.fao.org/aos/agrovoc/c_2604 http://aims.fao.org/aos/agrovoc/c_29496 http://aims.fao.org/aos/agrovoc/c_36638 http://aims.fao.org/aos/agrovoc/c_37481 http://aims.fao.org/aos/agrovoc/c_5791 |
author |
Ben Romdhane, Walid Chtourou, Yassine Sebii, Haifa Baklouti, Emna Nasri, Ameni Drira, Riadh Maalej, Mohamed Drira, Noureddine Rival, Alain Fki, Lotfi |
author_facet |
Ben Romdhane, Walid Chtourou, Yassine Sebii, Haifa Baklouti, Emna Nasri, Ameni Drira, Riadh Maalej, Mohamed Drira, Noureddine Rival, Alain Fki, Lotfi |
author_sort |
Ben Romdhane, Walid |
title |
Methyl jasmonate induces oxidative/nitrosative stress and the accumulation of antioxidant metabolites in Phoenix dactylifera L. |
title_short |
Methyl jasmonate induces oxidative/nitrosative stress and the accumulation of antioxidant metabolites in Phoenix dactylifera L. |
title_full |
Methyl jasmonate induces oxidative/nitrosative stress and the accumulation of antioxidant metabolites in Phoenix dactylifera L. |
title_fullStr |
Methyl jasmonate induces oxidative/nitrosative stress and the accumulation of antioxidant metabolites in Phoenix dactylifera L. |
title_full_unstemmed |
Methyl jasmonate induces oxidative/nitrosative stress and the accumulation of antioxidant metabolites in Phoenix dactylifera L. |
title_sort |
methyl jasmonate induces oxidative/nitrosative stress and the accumulation of antioxidant metabolites in phoenix dactylifera l. |
url |
http://agritrop.cirad.fr/604986/ http://agritrop.cirad.fr/604986/1/4a2ae22c-c578-446b-9ea3-7497e81eda40.PDF |
work_keys_str_mv |
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