CROWN ROOTLESS1 binds DNA with a relaxed specificity and activates OsROP and OsbHLH044 genes involved in crown root formation in rice

In cereals, the root system is mainly composed of post-embryonic shoot-borne roots, named crown roots. The CROWN ROOTLESS1 (CRL1) transcription factor, belonging to the ASYMMETRIC LEAVES2-LIKE/LATERAL ORGAN BOUNDARIES DOMAIN (ASL/LBD) family, is a key regulator of crown root initiation in rice (Oryza sativa). Here, we show that CRL1 can bind, both in vitro and in vivo, not only the LBD-box, a DNA sequence recognized by several ASL/LBD transcription factors, but also another not previously identified DNA motif that was named CRL1-box. Using rice protoplast transient transactivation assays and a set of previously identified CRL1-regulated genes, we confirm that CRL1 transactivates these genes if they possess at least a CRL1-box or an LBD-box in their promoters. In planta, ChIP-qPCR experiments targeting two of these genes that include both a CRL1- and an LBD-box in their promoter show that CRL1 binds preferentially to the LBD-box in these promoter contexts. CRISPR/Cas9-targeted mutation of these two CRL1-regulated genes, which encode a plant Rho GTPase (OsROP) and a basic helix–loop–helix transcription factor (OsbHLH044), show that both promote crown root development. Finally, we show that OsbHLH044 represses a regulatory module, uncovering how CRL1 regulates specific processes during crown root formation.

Bibliographic Details

Main Authors:	Gonin, Mathieu, Jeong, Kwanho, Coudert, Yoan, Lavarenne, Jeremy, Hoang, Giang Thi, Bes, Martine, To, Huong Thi Mai, Thiaw, Marie-Rose Ndella, Do, Toan Van, Moukouanga, Daniel, Guyomarc'h, Soazig, Bellande, Kevin, Brossier, Jean-Rémy, Parizot, Boris, Nguyen, Hieu Trang, Beeckman, Tom, Bergougnoux, Véronique, Rouster, Jacques, Sallaud, Christophe, Laplaze, Laurent, Champion, Anthony, Gantet, Pascal
Format:	article biblioteca
Language:	eng
Subjects:	Oryza sativa, système racinaire, Transcription génique, transcription d'ADN, adn, racine, expression des gènes, facteur de transcription, séquence nucléotidique, Houppier, http://aims.fao.org/aos/agrovoc/c_5438, http://aims.fao.org/aos/agrovoc/c_16034, http://aims.fao.org/aos/agrovoc/c_35128, http://aims.fao.org/aos/agrovoc/c_35127, http://aims.fao.org/aos/agrovoc/c_2347, http://aims.fao.org/aos/agrovoc/c_6651, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_35345, http://aims.fao.org/aos/agrovoc/c_27583, http://aims.fao.org/aos/agrovoc/c_16172,
Online Access:	http://agritrop.cirad.fr/604423/ http://agritrop.cirad.fr/604423/1/The%20Plant%20Journal%20-%202022%20-%20Gonin%20-%20CROWN%20ROOTLESS1%20binds%20DNA%20with%20a%20relaxed%20specificity%20and%20activates%20OsROP%20and%20OsbHLH044.pdf
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