Periodontitis associates with species-specific gene expression of the oral microbiota
The purpose of the present investigation was to characterize species-specific bacterial activity of the oral microbiota in periodontitis. We tested the hypotheses that chronic inflammation, i.e., periodontitis, associates with bacterial gene expression of the oral microbiota. Oral microbial samples were collected from three oral sites—subgingival plaque, tongue, and saliva from patients with periodontitis and healthy controls. Paired metagenomics and metatranscriptomics were used to perform concomitant characterization of taxonomic composition and to determine species-specific bacterial activity as expressed by the ratio of specific messenger RNA reads to their corresponding genomic DNA reads. Here, we show the association of periodontitis with bacterial gene expression of the oral microbiota. While oral site was the main determinant of taxonomic composition as well as bacterial gene expression, periodontitis was significantly associated with a reduction of carbohydrate metabolism of the oral microbiota at three oral sites (subgingival plaque, tongue, and saliva). Data from the present study revealed the association of periodontitis with bacterial gene expression of the oral microbiota. Conditions of periodontitis was associated with bacterial activity of local subgingival plaque, but also on tongue and the salivary microbiota. Collectively, data suggest that periodontitis associates with impaired carbohydrate metabolism of the oral microbiota. Future longitudinal and interventional studies are warranted to evaluate the potential pathogenic role of impaired bacterial carbohydrate metabolism not only in periodontitis but also in other diseases with low-grade inflammation, such as type 2 diabetes mellitus.
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Subjects: | flore microbienne, arn messager, expression des gènes, métabolisme des glucides, langue, écologie microbienne, adn, salive, gène, http://aims.fao.org/aos/agrovoc/c_16367, http://aims.fao.org/aos/agrovoc/c_4765, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_1299, http://aims.fao.org/aos/agrovoc/c_7809, http://aims.fao.org/aos/agrovoc/c_24111, http://aims.fao.org/aos/agrovoc/c_2347, http://aims.fao.org/aos/agrovoc/c_6752, http://aims.fao.org/aos/agrovoc/c_3214, |
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dig-cirad-fr-6034102024-04-25T08:30:17Z http://agritrop.cirad.fr/603410/ http://agritrop.cirad.fr/603410/ Periodontitis associates with species-specific gene expression of the oral microbiota. Belstrom Daniel, Constancias Florentin, Drautz-Moses Daniela I., Schuster Stephan C., Veleba Mark, Mahé Frédéric, Givskov Michael. 2021. npj Biofilms and Microbiomes, 7:76, 10 p.https://doi.org/10.1038/s41522-021-00247-y <https://doi.org/10.1038/s41522-021-00247-y> Periodontitis associates with species-specific gene expression of the oral microbiota Belstrom, Daniel Constancias, Florentin Drautz-Moses, Daniela I. Schuster, Stephan C. Veleba, Mark Mahé, Frédéric Givskov, Michael eng 2021 npj Biofilms and Microbiomes flore microbienne arn messager expression des gènes métabolisme des glucides langue écologie microbienne adn salive gène http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_4765 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_1299 http://aims.fao.org/aos/agrovoc/c_7809 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_6752 http://aims.fao.org/aos/agrovoc/c_3214 The purpose of the present investigation was to characterize species-specific bacterial activity of the oral microbiota in periodontitis. We tested the hypotheses that chronic inflammation, i.e., periodontitis, associates with bacterial gene expression of the oral microbiota. Oral microbial samples were collected from three oral sites—subgingival plaque, tongue, and saliva from patients with periodontitis and healthy controls. Paired metagenomics and metatranscriptomics were used to perform concomitant characterization of taxonomic composition and to determine species-specific bacterial activity as expressed by the ratio of specific messenger RNA reads to their corresponding genomic DNA reads. Here, we show the association of periodontitis with bacterial gene expression of the oral microbiota. While oral site was the main determinant of taxonomic composition as well as bacterial gene expression, periodontitis was significantly associated with a reduction of carbohydrate metabolism of the oral microbiota at three oral sites (subgingival plaque, tongue, and saliva). Data from the present study revealed the association of periodontitis with bacterial gene expression of the oral microbiota. Conditions of periodontitis was associated with bacterial activity of local subgingival plaque, but also on tongue and the salivary microbiota. Collectively, data suggest that periodontitis associates with impaired carbohydrate metabolism of the oral microbiota. Future longitudinal and interventional studies are warranted to evaluate the potential pathogenic role of impaired bacterial carbohydrate metabolism not only in periodontitis but also in other diseases with low-grade inflammation, such as type 2 diabetes mellitus. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/603410/1/s41522-021-00247-y.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.1038/s41522-021-00247-y 10.1038/s41522-021-00247-y info:eu-repo/semantics/altIdentifier/doi/10.1038/s41522-021-00247-y info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1038/s41522-021-00247-y info:eu-repo/semantics/reference/purl/https://github.com/fconstancias/oral-microbiome/ |
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flore microbienne arn messager expression des gènes métabolisme des glucides langue écologie microbienne adn salive gène http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_4765 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_1299 http://aims.fao.org/aos/agrovoc/c_7809 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_6752 http://aims.fao.org/aos/agrovoc/c_3214 flore microbienne arn messager expression des gènes métabolisme des glucides langue écologie microbienne adn salive gène http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_4765 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_1299 http://aims.fao.org/aos/agrovoc/c_7809 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_6752 http://aims.fao.org/aos/agrovoc/c_3214 |
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flore microbienne arn messager expression des gènes métabolisme des glucides langue écologie microbienne adn salive gène http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_4765 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_1299 http://aims.fao.org/aos/agrovoc/c_7809 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_6752 http://aims.fao.org/aos/agrovoc/c_3214 flore microbienne arn messager expression des gènes métabolisme des glucides langue écologie microbienne adn salive gène http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_4765 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_1299 http://aims.fao.org/aos/agrovoc/c_7809 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_6752 http://aims.fao.org/aos/agrovoc/c_3214 Belstrom, Daniel Constancias, Florentin Drautz-Moses, Daniela I. Schuster, Stephan C. Veleba, Mark Mahé, Frédéric Givskov, Michael Periodontitis associates with species-specific gene expression of the oral microbiota |
description |
The purpose of the present investigation was to characterize species-specific bacterial activity of the oral microbiota in periodontitis. We tested the hypotheses that chronic inflammation, i.e., periodontitis, associates with bacterial gene expression of the oral microbiota. Oral microbial samples were collected from three oral sites—subgingival plaque, tongue, and saliva from patients with periodontitis and healthy controls. Paired metagenomics and metatranscriptomics were used to perform concomitant characterization of taxonomic composition and to determine species-specific bacterial activity as expressed by the ratio of specific messenger RNA reads to their corresponding genomic DNA reads. Here, we show the association of periodontitis with bacterial gene expression of the oral microbiota. While oral site was the main determinant of taxonomic composition as well as bacterial gene expression, periodontitis was significantly associated with a reduction of carbohydrate metabolism of the oral microbiota at three oral sites (subgingival plaque, tongue, and saliva). Data from the present study revealed the association of periodontitis with bacterial gene expression of the oral microbiota. Conditions of periodontitis was associated with bacterial activity of local subgingival plaque, but also on tongue and the salivary microbiota. Collectively, data suggest that periodontitis associates with impaired carbohydrate metabolism of the oral microbiota. Future longitudinal and interventional studies are warranted to evaluate the potential pathogenic role of impaired bacterial carbohydrate metabolism not only in periodontitis but also in other diseases with low-grade inflammation, such as type 2 diabetes mellitus. |
format |
article |
topic_facet |
flore microbienne arn messager expression des gènes métabolisme des glucides langue écologie microbienne adn salive gène http://aims.fao.org/aos/agrovoc/c_16367 http://aims.fao.org/aos/agrovoc/c_4765 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_1299 http://aims.fao.org/aos/agrovoc/c_7809 http://aims.fao.org/aos/agrovoc/c_24111 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_6752 http://aims.fao.org/aos/agrovoc/c_3214 |
author |
Belstrom, Daniel Constancias, Florentin Drautz-Moses, Daniela I. Schuster, Stephan C. Veleba, Mark Mahé, Frédéric Givskov, Michael |
author_facet |
Belstrom, Daniel Constancias, Florentin Drautz-Moses, Daniela I. Schuster, Stephan C. Veleba, Mark Mahé, Frédéric Givskov, Michael |
author_sort |
Belstrom, Daniel |
title |
Periodontitis associates with species-specific gene expression of the oral microbiota |
title_short |
Periodontitis associates with species-specific gene expression of the oral microbiota |
title_full |
Periodontitis associates with species-specific gene expression of the oral microbiota |
title_fullStr |
Periodontitis associates with species-specific gene expression of the oral microbiota |
title_full_unstemmed |
Periodontitis associates with species-specific gene expression of the oral microbiota |
title_sort |
periodontitis associates with species-specific gene expression of the oral microbiota |
url |
http://agritrop.cirad.fr/603410/ http://agritrop.cirad.fr/603410/1/s41522-021-00247-y.pdf |
work_keys_str_mv |
AT belstromdaniel periodontitisassociateswithspeciesspecificgeneexpressionoftheoralmicrobiota AT constanciasflorentin periodontitisassociateswithspeciesspecificgeneexpressionoftheoralmicrobiota AT drautzmosesdanielai periodontitisassociateswithspeciesspecificgeneexpressionoftheoralmicrobiota AT schusterstephanc periodontitisassociateswithspeciesspecificgeneexpressionoftheoralmicrobiota AT velebamark periodontitisassociateswithspeciesspecificgeneexpressionoftheoralmicrobiota AT mahefrederic periodontitisassociateswithspeciesspecificgeneexpressionoftheoralmicrobiota AT givskovmichael periodontitisassociateswithspeciesspecificgeneexpressionoftheoralmicrobiota |
_version_ |
1798165091152035840 |