Vector competence of the African argasid tick Ornithodoros moubata for the Q fever agent Coxiella burnetii
Q fever is a widespread zoonotic disease caused by the intracellular bacterium Coxiella burnetii. While transmission is primarily but not exclusively airborne, ticks are usually thought to act as vectors on the basis of early microscopy studies. However, recent observations revealed that endosymbionts of ticks have been commonly misidentified as C. burnetii, calling the importance of tick-borne transmission into question. In this study, we re-evaluated the vector competence of the African soft tick Ornithodoros moubata for an avirulent strain of C. burnetii. To this end, we used an artificial feeding system to initiate infection of ticks, specific molecular tools to monitor further infections, and culture assays in axenic and cell media to check for the viability of C. burnetii excreted by ticks. We observed typical traits associated with vector competence: The exposure to an infected blood meal resulted in viable and persistent infections in ticks, trans-stadial transmissions of infection from nymphs to adults and the ability of adult ticks to transmit infectious C. burnetii. However, in contrast to early studies, we found that infection differed substantially between tick organs. In addition, while adult female ticks were infected, we did not observe C. burnetii in eggs, suggesting that transovarial transmission is not effective. Finally, we detected only a sporadic presence of C. burnetii DNA in tick faeces, but no living bacterium was further isolated in culture assays, suggesting that excretion in faeces is not a common mode of transmission in O. moubata.
Main Authors: | , , , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | L72 - Organismes nuisibles des animaux, L73 - Maladies des animaux, transmission des maladies, vecteur de maladie, fièvre Q, Ornithodoros moubata, Coxiella burnetii, http://aims.fao.org/aos/agrovoc/c_2329, http://aims.fao.org/aos/agrovoc/c_8164, http://aims.fao.org/aos/agrovoc/c_16461, http://aims.fao.org/aos/agrovoc/c_30948, http://aims.fao.org/aos/agrovoc/c_36027, |
Online Access: | http://agritrop.cirad.fr/601537/ http://agritrop.cirad.fr/601537/1/journal.pntd.0009008.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-601537 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-6015372024-01-29T04:16:23Z http://agritrop.cirad.fr/601537/ http://agritrop.cirad.fr/601537/ Vector competence of the African argasid tick Ornithodoros moubata for the Q fever agent Coxiella burnetii. Buysse Marie, Duhayon Maxime, Cantet Franck, Bonazzi Matteo, Duron Olivier. 2021. PLoS Neglected Tropical Diseases, 15 (1):e0009008, 14 p.https://doi.org/10.1371/journal.pntd.0009008 <https://doi.org/10.1371/journal.pntd.0009008> Vector competence of the African argasid tick Ornithodoros moubata for the Q fever agent Coxiella burnetii Buysse, Marie Duhayon, Maxime Cantet, Franck Bonazzi, Matteo Duron, Olivier eng 2021 PLoS Neglected Tropical Diseases L72 - Organismes nuisibles des animaux L73 - Maladies des animaux transmission des maladies vecteur de maladie fièvre Q Ornithodoros moubata Coxiella burnetii http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_16461 http://aims.fao.org/aos/agrovoc/c_30948 http://aims.fao.org/aos/agrovoc/c_36027 Q fever is a widespread zoonotic disease caused by the intracellular bacterium Coxiella burnetii. While transmission is primarily but not exclusively airborne, ticks are usually thought to act as vectors on the basis of early microscopy studies. However, recent observations revealed that endosymbionts of ticks have been commonly misidentified as C. burnetii, calling the importance of tick-borne transmission into question. In this study, we re-evaluated the vector competence of the African soft tick Ornithodoros moubata for an avirulent strain of C. burnetii. To this end, we used an artificial feeding system to initiate infection of ticks, specific molecular tools to monitor further infections, and culture assays in axenic and cell media to check for the viability of C. burnetii excreted by ticks. We observed typical traits associated with vector competence: The exposure to an infected blood meal resulted in viable and persistent infections in ticks, trans-stadial transmissions of infection from nymphs to adults and the ability of adult ticks to transmit infectious C. burnetii. However, in contrast to early studies, we found that infection differed substantially between tick organs. In addition, while adult female ticks were infected, we did not observe C. burnetii in eggs, suggesting that transovarial transmission is not effective. Finally, we detected only a sporadic presence of C. burnetii DNA in tick faeces, but no living bacterium was further isolated in culture assays, suggesting that excretion in faeces is not a common mode of transmission in O. moubata. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/601537/1/journal.pntd.0009008.pdf text cc_by info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/4.0/ https://doi.org/10.1371/journal.pntd.0009008 10.1371/journal.pntd.0009008 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pntd.0009008 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1371/journal.pntd.0009008 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
L72 - Organismes nuisibles des animaux L73 - Maladies des animaux transmission des maladies vecteur de maladie fièvre Q Ornithodoros moubata Coxiella burnetii http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_16461 http://aims.fao.org/aos/agrovoc/c_30948 http://aims.fao.org/aos/agrovoc/c_36027 L72 - Organismes nuisibles des animaux L73 - Maladies des animaux transmission des maladies vecteur de maladie fièvre Q Ornithodoros moubata Coxiella burnetii http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_16461 http://aims.fao.org/aos/agrovoc/c_30948 http://aims.fao.org/aos/agrovoc/c_36027 |
spellingShingle |
L72 - Organismes nuisibles des animaux L73 - Maladies des animaux transmission des maladies vecteur de maladie fièvre Q Ornithodoros moubata Coxiella burnetii http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_16461 http://aims.fao.org/aos/agrovoc/c_30948 http://aims.fao.org/aos/agrovoc/c_36027 L72 - Organismes nuisibles des animaux L73 - Maladies des animaux transmission des maladies vecteur de maladie fièvre Q Ornithodoros moubata Coxiella burnetii http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_16461 http://aims.fao.org/aos/agrovoc/c_30948 http://aims.fao.org/aos/agrovoc/c_36027 Buysse, Marie Duhayon, Maxime Cantet, Franck Bonazzi, Matteo Duron, Olivier Vector competence of the African argasid tick Ornithodoros moubata for the Q fever agent Coxiella burnetii |
description |
Q fever is a widespread zoonotic disease caused by the intracellular bacterium Coxiella burnetii. While transmission is primarily but not exclusively airborne, ticks are usually thought to act as vectors on the basis of early microscopy studies. However, recent observations revealed that endosymbionts of ticks have been commonly misidentified as C. burnetii, calling the importance of tick-borne transmission into question. In this study, we re-evaluated the vector competence of the African soft tick Ornithodoros moubata for an avirulent strain of C. burnetii. To this end, we used an artificial feeding system to initiate infection of ticks, specific molecular tools to monitor further infections, and culture assays in axenic and cell media to check for the viability of C. burnetii excreted by ticks. We observed typical traits associated with vector competence: The exposure to an infected blood meal resulted in viable and persistent infections in ticks, trans-stadial transmissions of infection from nymphs to adults and the ability of adult ticks to transmit infectious C. burnetii. However, in contrast to early studies, we found that infection differed substantially between tick organs. In addition, while adult female ticks were infected, we did not observe C. burnetii in eggs, suggesting that transovarial transmission is not effective. Finally, we detected only a sporadic presence of C. burnetii DNA in tick faeces, but no living bacterium was further isolated in culture assays, suggesting that excretion in faeces is not a common mode of transmission in O. moubata. |
format |
article |
topic_facet |
L72 - Organismes nuisibles des animaux L73 - Maladies des animaux transmission des maladies vecteur de maladie fièvre Q Ornithodoros moubata Coxiella burnetii http://aims.fao.org/aos/agrovoc/c_2329 http://aims.fao.org/aos/agrovoc/c_8164 http://aims.fao.org/aos/agrovoc/c_16461 http://aims.fao.org/aos/agrovoc/c_30948 http://aims.fao.org/aos/agrovoc/c_36027 |
author |
Buysse, Marie Duhayon, Maxime Cantet, Franck Bonazzi, Matteo Duron, Olivier |
author_facet |
Buysse, Marie Duhayon, Maxime Cantet, Franck Bonazzi, Matteo Duron, Olivier |
author_sort |
Buysse, Marie |
title |
Vector competence of the African argasid tick Ornithodoros moubata for the Q fever agent Coxiella burnetii |
title_short |
Vector competence of the African argasid tick Ornithodoros moubata for the Q fever agent Coxiella burnetii |
title_full |
Vector competence of the African argasid tick Ornithodoros moubata for the Q fever agent Coxiella burnetii |
title_fullStr |
Vector competence of the African argasid tick Ornithodoros moubata for the Q fever agent Coxiella burnetii |
title_full_unstemmed |
Vector competence of the African argasid tick Ornithodoros moubata for the Q fever agent Coxiella burnetii |
title_sort |
vector competence of the african argasid tick ornithodoros moubata for the q fever agent coxiella burnetii |
url |
http://agritrop.cirad.fr/601537/ http://agritrop.cirad.fr/601537/1/journal.pntd.0009008.pdf |
work_keys_str_mv |
AT buyssemarie vectorcompetenceoftheafricanargasidtickornithodorosmoubatafortheqfeveragentcoxiellaburnetii AT duhayonmaxime vectorcompetenceoftheafricanargasidtickornithodorosmoubatafortheqfeveragentcoxiellaburnetii AT cantetfranck vectorcompetenceoftheafricanargasidtickornithodorosmoubatafortheqfeveragentcoxiellaburnetii AT bonazzimatteo vectorcompetenceoftheafricanargasidtickornithodorosmoubatafortheqfeveragentcoxiellaburnetii AT duronolivier vectorcompetenceoftheafricanargasidtickornithodorosmoubatafortheqfeveragentcoxiellaburnetii |
_version_ |
1792500390330105856 |