Transcriptomic analysis of the sugarcane orange rust pathogen Puccinia kuehnii
Puccinia kuehnii, the causal agent of orange rust, is a fungal pathogen that induced significant losses to the sugarcane crop in Florida since 2007. During plant infection, rust fungi deliver effector proteins into host tissues to manipulate plant functions and promote parasitic growth. Identiflcation and functional characterization of secreted effectors are essential steps to understand the molecular basis of host-pathogen interactions. Like other biotrophic fungi, Puccinia species possess large repertoires of secreted proteins whose vast majority has no known function.The objective of our investigations is to identify genes encoding secreted proteins, including relevant disease-related candidate effectors that are expressed during sugarcane infection by the orange rust pathogen. In a first experiment, healthy leaves of sugarcane cultivars CPB9-2743 (susceptible to orange rust) and CP96-1252 (resistant) were inoculated with race 2743 of P. kuehnii. In a second experiment, healthy leaves of sugarcane cultivars CL85-1040 (susceptible) and CP96-1,252 (resistant) were inoculated with race 1040 of the pathogen. Total RNA was extracted at 0-72 days after inoculation and transcript expression was quantilified through Illumina RNA-sequencing. Differential gene expression profiling was then determined for the compatible and incompatible interactions using DESeq2. Ongoing work includes characterization of effector proteins differentiating races 1040 and 2743 of P. kuehnii. Our longterm goal is to identify a set of candidate effector proteins for identification of host defense related proteins in marker-assisted breeding of sugarcane.
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Format: | article biblioteca |
Language: | eng |
Subjects: | H20 - Maladies des plantes, F30 - Génétique et amélioration des plantes, Puccinia, gène, marqueur génétique, protéine microbiologique, effecteur moléculaire, infection, rouille, maladie fongique, maladie foliaire, Puccinia kuehnii, http://aims.fao.org/aos/agrovoc/c_6354, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_24030, http://aims.fao.org/aos/agrovoc/c_7078, http://aims.fao.org/aos/agrovoc/c_27506, http://aims.fao.org/aos/agrovoc/c_3852, http://aims.fao.org/aos/agrovoc/c_6710, http://aims.fao.org/aos/agrovoc/c_11042, http://aims.fao.org/aos/agrovoc/c_25240, http://aims.fao.org/aos/agrovoc/c_ac7b37ec, |
Online Access: | http://agritrop.cirad.fr/601322/ http://agritrop.cirad.fr/601322/1/Cardenas-Transcriptomic%20analysis%20Puccinia%20kuehnii_Sugar%20Journal%20June%202022.pdf |
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dig-cirad-fr-6013222024-01-29T04:13:56Z http://agritrop.cirad.fr/601322/ http://agritrop.cirad.fr/601322/ Transcriptomic analysis of the sugarcane orange rust pathogen Puccinia kuehnii. Cardenas Daniela E., Auer Lucas, Morin Emmanuelle, Carrillo-Tarazona Yisel, Sood Sushma, Huguet-Tapia Jose, Saunders Diane G.O., Duplessis Sébastien, Rott Philippe, Cano Liliana M.. 2022. Sugar Journal (June) : 33-34. ASSCT 2022 Annual Meeting, Bonita Springs, États-Unis, 21 Juin 2022/23 Juin 2022.https://www.sugarjournal.com/ <https://www.sugarjournal.com/> Transcriptomic analysis of the sugarcane orange rust pathogen Puccinia kuehnii Cardenas, Daniela E. Auer, Lucas Morin, Emmanuelle Carrillo-Tarazona, Yisel Sood, Sushma Huguet-Tapia, Jose Saunders, Diane G.O. Duplessis, Sébastien Rott, Philippe Cano, Liliana M. eng 2022 Sugar Journal H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Puccinia gène marqueur génétique protéine microbiologique effecteur moléculaire infection rouille maladie fongique maladie foliaire Puccinia kuehnii http://aims.fao.org/aos/agrovoc/c_6354 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7078 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_6710 http://aims.fao.org/aos/agrovoc/c_11042 http://aims.fao.org/aos/agrovoc/c_25240 http://aims.fao.org/aos/agrovoc/c_ac7b37ec Puccinia kuehnii, the causal agent of orange rust, is a fungal pathogen that induced significant losses to the sugarcane crop in Florida since 2007. During plant infection, rust fungi deliver effector proteins into host tissues to manipulate plant functions and promote parasitic growth. Identiflcation and functional characterization of secreted effectors are essential steps to understand the molecular basis of host-pathogen interactions. Like other biotrophic fungi, Puccinia species possess large repertoires of secreted proteins whose vast majority has no known function.The objective of our investigations is to identify genes encoding secreted proteins, including relevant disease-related candidate effectors that are expressed during sugarcane infection by the orange rust pathogen. In a first experiment, healthy leaves of sugarcane cultivars CPB9-2743 (susceptible to orange rust) and CP96-1252 (resistant) were inoculated with race 2743 of P. kuehnii. In a second experiment, healthy leaves of sugarcane cultivars CL85-1040 (susceptible) and CP96-1,252 (resistant) were inoculated with race 1040 of the pathogen. Total RNA was extracted at 0-72 days after inoculation and transcript expression was quantilified through Illumina RNA-sequencing. Differential gene expression profiling was then determined for the compatible and incompatible interactions using DESeq2. Ongoing work includes characterization of effector proteins differentiating races 1040 and 2743 of P. kuehnii. Our longterm goal is to identify a set of candidate effector proteins for identification of host defense related proteins in marker-assisted breeding of sugarcane. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/601322/1/Cardenas-Transcriptomic%20analysis%20Puccinia%20kuehnii_Sugar%20Journal%20June%202022.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://www.sugarjournal.com/ info:eu-repo/semantics/altIdentifier/purl/https://www.sugarjournal.com/ |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Puccinia gène marqueur génétique protéine microbiologique effecteur moléculaire infection rouille maladie fongique maladie foliaire Puccinia kuehnii http://aims.fao.org/aos/agrovoc/c_6354 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7078 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_6710 http://aims.fao.org/aos/agrovoc/c_11042 http://aims.fao.org/aos/agrovoc/c_25240 http://aims.fao.org/aos/agrovoc/c_ac7b37ec H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Puccinia gène marqueur génétique protéine microbiologique effecteur moléculaire infection rouille maladie fongique maladie foliaire Puccinia kuehnii http://aims.fao.org/aos/agrovoc/c_6354 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7078 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_6710 http://aims.fao.org/aos/agrovoc/c_11042 http://aims.fao.org/aos/agrovoc/c_25240 http://aims.fao.org/aos/agrovoc/c_ac7b37ec |
spellingShingle |
H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Puccinia gène marqueur génétique protéine microbiologique effecteur moléculaire infection rouille maladie fongique maladie foliaire Puccinia kuehnii http://aims.fao.org/aos/agrovoc/c_6354 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7078 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_6710 http://aims.fao.org/aos/agrovoc/c_11042 http://aims.fao.org/aos/agrovoc/c_25240 http://aims.fao.org/aos/agrovoc/c_ac7b37ec H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Puccinia gène marqueur génétique protéine microbiologique effecteur moléculaire infection rouille maladie fongique maladie foliaire Puccinia kuehnii http://aims.fao.org/aos/agrovoc/c_6354 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7078 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_6710 http://aims.fao.org/aos/agrovoc/c_11042 http://aims.fao.org/aos/agrovoc/c_25240 http://aims.fao.org/aos/agrovoc/c_ac7b37ec Cardenas, Daniela E. Auer, Lucas Morin, Emmanuelle Carrillo-Tarazona, Yisel Sood, Sushma Huguet-Tapia, Jose Saunders, Diane G.O. Duplessis, Sébastien Rott, Philippe Cano, Liliana M. Transcriptomic analysis of the sugarcane orange rust pathogen Puccinia kuehnii |
description |
Puccinia kuehnii, the causal agent of orange rust, is a fungal pathogen that induced significant losses to the sugarcane crop in Florida since 2007. During plant infection, rust fungi deliver effector proteins into host tissues to manipulate plant functions and promote parasitic growth. Identiflcation and functional characterization of secreted effectors are essential steps to understand the molecular basis of host-pathogen interactions. Like other biotrophic fungi, Puccinia species possess large repertoires of secreted proteins whose vast majority has no known function.The objective of our investigations is to identify genes encoding secreted proteins, including relevant disease-related candidate effectors that are expressed during sugarcane infection by the orange rust pathogen. In a first experiment, healthy leaves of sugarcane cultivars CPB9-2743 (susceptible to orange rust) and CP96-1252 (resistant) were inoculated with race 2743 of P. kuehnii. In a second experiment, healthy leaves of sugarcane cultivars CL85-1040 (susceptible) and CP96-1,252 (resistant) were inoculated with race 1040 of the pathogen. Total RNA was extracted at 0-72 days after inoculation and transcript expression was quantilified through Illumina RNA-sequencing. Differential gene expression profiling was then determined for the compatible and incompatible interactions using DESeq2. Ongoing work includes characterization of effector proteins differentiating races 1040 and 2743 of P. kuehnii. Our longterm goal is to identify a set of candidate effector proteins for identification of host defense related proteins in marker-assisted breeding of sugarcane. |
format |
article |
topic_facet |
H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Puccinia gène marqueur génétique protéine microbiologique effecteur moléculaire infection rouille maladie fongique maladie foliaire Puccinia kuehnii http://aims.fao.org/aos/agrovoc/c_6354 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_7078 http://aims.fao.org/aos/agrovoc/c_27506 http://aims.fao.org/aos/agrovoc/c_3852 http://aims.fao.org/aos/agrovoc/c_6710 http://aims.fao.org/aos/agrovoc/c_11042 http://aims.fao.org/aos/agrovoc/c_25240 http://aims.fao.org/aos/agrovoc/c_ac7b37ec |
author |
Cardenas, Daniela E. Auer, Lucas Morin, Emmanuelle Carrillo-Tarazona, Yisel Sood, Sushma Huguet-Tapia, Jose Saunders, Diane G.O. Duplessis, Sébastien Rott, Philippe Cano, Liliana M. |
author_facet |
Cardenas, Daniela E. Auer, Lucas Morin, Emmanuelle Carrillo-Tarazona, Yisel Sood, Sushma Huguet-Tapia, Jose Saunders, Diane G.O. Duplessis, Sébastien Rott, Philippe Cano, Liliana M. |
author_sort |
Cardenas, Daniela E. |
title |
Transcriptomic analysis of the sugarcane orange rust pathogen Puccinia kuehnii |
title_short |
Transcriptomic analysis of the sugarcane orange rust pathogen Puccinia kuehnii |
title_full |
Transcriptomic analysis of the sugarcane orange rust pathogen Puccinia kuehnii |
title_fullStr |
Transcriptomic analysis of the sugarcane orange rust pathogen Puccinia kuehnii |
title_full_unstemmed |
Transcriptomic analysis of the sugarcane orange rust pathogen Puccinia kuehnii |
title_sort |
transcriptomic analysis of the sugarcane orange rust pathogen puccinia kuehnii |
url |
http://agritrop.cirad.fr/601322/ http://agritrop.cirad.fr/601322/1/Cardenas-Transcriptomic%20analysis%20Puccinia%20kuehnii_Sugar%20Journal%20June%202022.pdf |
work_keys_str_mv |
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_version_ |
1792500376542380032 |