Phenotypic and genetic characterization of MERS coronaviruses from Africa to understand their zoonotic potential
Coronaviruses are pathogens of pandemic potential. Middle East respiratory syndrome coronavirus (MERS-CoV) causes a zoonotic respiratory disease of global public health concern, and dromedary camels are the only proven source of zoonotic infection. More than 70% of MERS-CoV–infected dromedaries are found in East, North, and West Africa, but zoonotic MERS disease is only reported from the Arabian Peninsula. We compared viral replication competence of clade A and B viruses from the Arabian Peninsula with genetically diverse clade C viruses found in East (Egypt, Kenya, and Ethiopia), North (Morocco), and West (Nigeria and Burkina Faso) Africa. Viruses from Africa had lower replication competence in ex vivo cultures of the human lung and in lungs of experimentally infected human-DPP4 (hDPP4) knockin mice. We used lentivirus pseudotypes expressing MERS-CoV spike from Saudi Arabian clade A prototype strain (EMC) or African clade C1.1 viruses and demonstrated that clade C1.1 spike was associated with reduced virus entry into the respiratory epithelial cell line Calu-3. Isogenic EMC viruses with spike protein from EMC or clade C1.1 generated by reverse genetics showed that the clade C1.1 spike was associated with reduced virus replication competence in Calu-3 cells in vitro, in ex vivo human bronchus, and in lungs of hDPP4 knockin mice in vivo. These findings may explain why zoonotic MERS disease has not been reported from Africa so far, despite exposure to and infection with MERS-CoV.
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S50 - Santé humaine L73 - Maladies des animaux pouvoir pathogène Coronavirus du syndrome respiratoire du Moyen-Orient zoonose surveillance épidémiologique paramètre génétique variation phénotypique phénotype Coronaviridae http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_8e19eb98 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_24847 http://aims.fao.org/aos/agrovoc/c_903c4959 http://aims.fao.org/aos/agrovoc/c_5776 http://aims.fao.org/aos/agrovoc/c_1902 http://aims.fao.org/aos/agrovoc/c_2503 http://aims.fao.org/aos/agrovoc/c_4086 http://aims.fao.org/aos/agrovoc/c_2676 http://aims.fao.org/aos/agrovoc/c_4940 http://aims.fao.org/aos/agrovoc/c_5182 http://aims.fao.org/aos/agrovoc/c_8081 S50 - Santé humaine L73 - Maladies des animaux pouvoir pathogène Coronavirus du syndrome respiratoire du Moyen-Orient zoonose surveillance épidémiologique paramètre génétique variation phénotypique phénotype Coronaviridae http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_8e19eb98 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_24847 http://aims.fao.org/aos/agrovoc/c_903c4959 http://aims.fao.org/aos/agrovoc/c_5776 http://aims.fao.org/aos/agrovoc/c_1902 http://aims.fao.org/aos/agrovoc/c_2503 http://aims.fao.org/aos/agrovoc/c_4086 http://aims.fao.org/aos/agrovoc/c_2676 http://aims.fao.org/aos/agrovoc/c_4940 http://aims.fao.org/aos/agrovoc/c_5182 http://aims.fao.org/aos/agrovoc/c_8081 |
spellingShingle |
S50 - Santé humaine L73 - Maladies des animaux pouvoir pathogène Coronavirus du syndrome respiratoire du Moyen-Orient zoonose surveillance épidémiologique paramètre génétique variation phénotypique phénotype Coronaviridae http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_8e19eb98 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_24847 http://aims.fao.org/aos/agrovoc/c_903c4959 http://aims.fao.org/aos/agrovoc/c_5776 http://aims.fao.org/aos/agrovoc/c_1902 http://aims.fao.org/aos/agrovoc/c_2503 http://aims.fao.org/aos/agrovoc/c_4086 http://aims.fao.org/aos/agrovoc/c_2676 http://aims.fao.org/aos/agrovoc/c_4940 http://aims.fao.org/aos/agrovoc/c_5182 http://aims.fao.org/aos/agrovoc/c_8081 S50 - Santé humaine L73 - Maladies des animaux pouvoir pathogène Coronavirus du syndrome respiratoire du Moyen-Orient zoonose surveillance épidémiologique paramètre génétique variation phénotypique phénotype Coronaviridae http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_8e19eb98 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_24847 http://aims.fao.org/aos/agrovoc/c_903c4959 http://aims.fao.org/aos/agrovoc/c_5776 http://aims.fao.org/aos/agrovoc/c_1902 http://aims.fao.org/aos/agrovoc/c_2503 http://aims.fao.org/aos/agrovoc/c_4086 http://aims.fao.org/aos/agrovoc/c_2676 http://aims.fao.org/aos/agrovoc/c_4940 http://aims.fao.org/aos/agrovoc/c_5182 http://aims.fao.org/aos/agrovoc/c_8081 Zhou, Ziqi Hui, Kenrie P.Y. So, Ray T.Y. Lv, Huibin Perera, Ranawaka A.P.M. Chu, Daniel K.W. Gelayas, Esayas Oyas, Harry Njagi, Obadiah Abayneh, Takele Kuria, Wilson Walelign, Elias Wanglia, Rinah El Masry, Ihab Von Dobschuetz, Sophie Kalpravidh, Wantanee Chevalier, Véronique Miguel, Eve Fassi-Fihri, Ouafaa Trarore, Amadou Liang, Weiwen Wang, Yanqun Nicholls, John M. Zhao, Jincun Chan, Michael C.W. Poon, Leo L.M. Mok, Chris Ka Pun Peiris, Malik Phenotypic and genetic characterization of MERS coronaviruses from Africa to understand their zoonotic potential |
description |
Coronaviruses are pathogens of pandemic potential. Middle East respiratory syndrome coronavirus (MERS-CoV) causes a zoonotic respiratory disease of global public health concern, and dromedary camels are the only proven source of zoonotic infection. More than 70% of MERS-CoV–infected dromedaries are found in East, North, and West Africa, but zoonotic MERS disease is only reported from the Arabian Peninsula. We compared viral replication competence of clade A and B viruses from the Arabian Peninsula with genetically diverse clade C viruses found in East (Egypt, Kenya, and Ethiopia), North (Morocco), and West (Nigeria and Burkina Faso) Africa. Viruses from Africa had lower replication competence in ex vivo cultures of the human lung and in lungs of experimentally infected human-DPP4 (hDPP4) knockin mice. We used lentivirus pseudotypes expressing MERS-CoV spike from Saudi Arabian clade A prototype strain (EMC) or African clade C1.1 viruses and demonstrated that clade C1.1 spike was associated with reduced virus entry into the respiratory epithelial cell line Calu-3. Isogenic EMC viruses with spike protein from EMC or clade C1.1 generated by reverse genetics showed that the clade C1.1 spike was associated with reduced virus replication competence in Calu-3 cells in vitro, in ex vivo human bronchus, and in lungs of hDPP4 knockin mice in vivo. These findings may explain why zoonotic MERS disease has not been reported from Africa so far, despite exposure to and infection with MERS-CoV. |
format |
article |
topic_facet |
S50 - Santé humaine L73 - Maladies des animaux pouvoir pathogène Coronavirus du syndrome respiratoire du Moyen-Orient zoonose surveillance épidémiologique paramètre génétique variation phénotypique phénotype Coronaviridae http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_8e19eb98 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_24847 http://aims.fao.org/aos/agrovoc/c_903c4959 http://aims.fao.org/aos/agrovoc/c_5776 http://aims.fao.org/aos/agrovoc/c_1902 http://aims.fao.org/aos/agrovoc/c_2503 http://aims.fao.org/aos/agrovoc/c_4086 http://aims.fao.org/aos/agrovoc/c_2676 http://aims.fao.org/aos/agrovoc/c_4940 http://aims.fao.org/aos/agrovoc/c_5182 http://aims.fao.org/aos/agrovoc/c_8081 |
author |
Zhou, Ziqi Hui, Kenrie P.Y. So, Ray T.Y. Lv, Huibin Perera, Ranawaka A.P.M. Chu, Daniel K.W. Gelayas, Esayas Oyas, Harry Njagi, Obadiah Abayneh, Takele Kuria, Wilson Walelign, Elias Wanglia, Rinah El Masry, Ihab Von Dobschuetz, Sophie Kalpravidh, Wantanee Chevalier, Véronique Miguel, Eve Fassi-Fihri, Ouafaa Trarore, Amadou Liang, Weiwen Wang, Yanqun Nicholls, John M. Zhao, Jincun Chan, Michael C.W. Poon, Leo L.M. Mok, Chris Ka Pun Peiris, Malik |
author_facet |
Zhou, Ziqi Hui, Kenrie P.Y. So, Ray T.Y. Lv, Huibin Perera, Ranawaka A.P.M. Chu, Daniel K.W. Gelayas, Esayas Oyas, Harry Njagi, Obadiah Abayneh, Takele Kuria, Wilson Walelign, Elias Wanglia, Rinah El Masry, Ihab Von Dobschuetz, Sophie Kalpravidh, Wantanee Chevalier, Véronique Miguel, Eve Fassi-Fihri, Ouafaa Trarore, Amadou Liang, Weiwen Wang, Yanqun Nicholls, John M. Zhao, Jincun Chan, Michael C.W. Poon, Leo L.M. Mok, Chris Ka Pun Peiris, Malik |
author_sort |
Zhou, Ziqi |
title |
Phenotypic and genetic characterization of MERS coronaviruses from Africa to understand their zoonotic potential |
title_short |
Phenotypic and genetic characterization of MERS coronaviruses from Africa to understand their zoonotic potential |
title_full |
Phenotypic and genetic characterization of MERS coronaviruses from Africa to understand their zoonotic potential |
title_fullStr |
Phenotypic and genetic characterization of MERS coronaviruses from Africa to understand their zoonotic potential |
title_full_unstemmed |
Phenotypic and genetic characterization of MERS coronaviruses from Africa to understand their zoonotic potential |
title_sort |
phenotypic and genetic characterization of mers coronaviruses from africa to understand their zoonotic potential |
url |
http://agritrop.cirad.fr/601033/ http://agritrop.cirad.fr/601033/1/601033.pdf |
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dig-cirad-fr-6010332024-05-02T14:14:13Z http://agritrop.cirad.fr/601033/ http://agritrop.cirad.fr/601033/ Phenotypic and genetic characterization of MERS coronaviruses from Africa to understand their zoonotic potential. Zhou Ziqi, Hui Kenrie P.Y., So Ray T.Y., Lv Huibin, Perera Ranawaka A.P.M., Chu Daniel K.W., Gelayas Esayas, Oyas Harry, Njagi Obadiah, Abayneh Takele, Kuria Wilson, Walelign Elias, Wanglia Rinah, El Masry Ihab, Von Dobschuetz Sophie, Kalpravidh Wantanee, Chevalier Véronique, Miguel Eve, Fassi-Fihri Ouafaa, Trarore Amadou, Liang Weiwen, Wang Yanqun, Nicholls John M., Zhao Jincun, Chan Michael C.W., Poon Leo L.M., Mok Chris Ka Pun, Peiris Malik. 2021. Proceedings of the National Academy of Sciences of the United States of America, 118 (25):e2103984118, 8 p.https://doi.org/10.1073/pnas.2103984118 <https://doi.org/10.1073/pnas.2103984118> Phenotypic and genetic characterization of MERS coronaviruses from Africa to understand their zoonotic potential Zhou, Ziqi Hui, Kenrie P.Y. So, Ray T.Y. Lv, Huibin Perera, Ranawaka A.P.M. Chu, Daniel K.W. Gelayas, Esayas Oyas, Harry Njagi, Obadiah Abayneh, Takele Kuria, Wilson Walelign, Elias Wanglia, Rinah El Masry, Ihab Von Dobschuetz, Sophie Kalpravidh, Wantanee Chevalier, Véronique Miguel, Eve Fassi-Fihri, Ouafaa Trarore, Amadou Liang, Weiwen Wang, Yanqun Nicholls, John M. Zhao, Jincun Chan, Michael C.W. Poon, Leo L.M. Mok, Chris Ka Pun Peiris, Malik eng 2021 Proceedings of the National Academy of Sciences of the United States of America S50 - Santé humaine L73 - Maladies des animaux pouvoir pathogène Coronavirus du syndrome respiratoire du Moyen-Orient zoonose surveillance épidémiologique paramètre génétique variation phénotypique phénotype Coronaviridae http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_8e19eb98 http://aims.fao.org/aos/agrovoc/c_8530 http://aims.fao.org/aos/agrovoc/c_16411 http://aims.fao.org/aos/agrovoc/c_24847 http://aims.fao.org/aos/agrovoc/c_903c4959 http://aims.fao.org/aos/agrovoc/c_5776 http://aims.fao.org/aos/agrovoc/c_1902 Égypte Kenya Éthiopie Maroc Nigéria Burkina Faso http://aims.fao.org/aos/agrovoc/c_2503 http://aims.fao.org/aos/agrovoc/c_4086 http://aims.fao.org/aos/agrovoc/c_2676 http://aims.fao.org/aos/agrovoc/c_4940 http://aims.fao.org/aos/agrovoc/c_5182 http://aims.fao.org/aos/agrovoc/c_8081 Coronaviruses are pathogens of pandemic potential. Middle East respiratory syndrome coronavirus (MERS-CoV) causes a zoonotic respiratory disease of global public health concern, and dromedary camels are the only proven source of zoonotic infection. More than 70% of MERS-CoV–infected dromedaries are found in East, North, and West Africa, but zoonotic MERS disease is only reported from the Arabian Peninsula. We compared viral replication competence of clade A and B viruses from the Arabian Peninsula with genetically diverse clade C viruses found in East (Egypt, Kenya, and Ethiopia), North (Morocco), and West (Nigeria and Burkina Faso) Africa. Viruses from Africa had lower replication competence in ex vivo cultures of the human lung and in lungs of experimentally infected human-DPP4 (hDPP4) knockin mice. We used lentivirus pseudotypes expressing MERS-CoV spike from Saudi Arabian clade A prototype strain (EMC) or African clade C1.1 viruses and demonstrated that clade C1.1 spike was associated with reduced virus entry into the respiratory epithelial cell line Calu-3. Isogenic EMC viruses with spike protein from EMC or clade C1.1 generated by reverse genetics showed that the clade C1.1 spike was associated with reduced virus replication competence in Calu-3 cells in vitro, in ex vivo human bronchus, and in lungs of hDPP4 knockin mice in vivo. These findings may explain why zoonotic MERS disease has not been reported from Africa so far, despite exposure to and infection with MERS-CoV. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/601033/1/601033.pdf text cc_by_nc_nd info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ https://doi.org/10.1073/pnas.2103984118 10.1073/pnas.2103984118 info:eu-repo/semantics/altIdentifier/doi/10.1073/pnas.2103984118 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1073/pnas.2103984118 |