Alfalfa leaf curl virus is efficiently acquired by its aphid vector Aphis craccivora but inefficiently transmitted
Alfalfa leaf curl virus (ALCV) is the first geminivirus for which aphid transmission was reported. Transmission by Aphis craccivora was determined previously to be highly specific and circulative. Using various complementary techniques, the transmission journey of ALCV was monitored from its uptake from infected plant tissues up to the head of its vector. ALCV was shown to be restricted to phloem tissues using fluorescence in situ hybridization (FISH) and electropenetrography (EPG) monitoring of virus acquisition. Furthermore, the virus is heterogeneously distributed in phloem tissues, as revealed by FISH and quantitative PCR of viral DNA acquired by EPG-monitored aphids. Despite the efficient ingestion of viral DNA, about 106 viral DNA copies per insect in a 15 h feeding period on ALCV-infected plants, the individual maximum transmission rate was 12 %. Transmission success was related to a critical viral accumulation, around 1.6×107 viral DNA copies per insect, a threshold that generally needed more than 48 h to be reached. Moreover, whereas the amount of acquired virus did not decrease over time in the whole aphid body, it declined in the haemolymph and heads. ALCV was not detected in progenies of viruliferous aphids and did not affect aphid fitness. Compared to geminiviruses transmitted by whiteflies or leafhoppers, or to luteoviruses transmitted by aphids, the transmission efficiency of ALCV by A. craccivora is low. This result is discussed in relation to the aphid vector of this geminivirus and the agroecological features of alfalfa, a hardy perennial host plant.
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Subjects: | H20 - Maladies des plantes, enroulement des feuilles, Aphis craccivora, vecteur de maladie, http://aims.fao.org/aos/agrovoc/c_4237, http://aims.fao.org/aos/agrovoc/c_30760, http://aims.fao.org/aos/agrovoc/c_8164, |
Online Access: | http://agritrop.cirad.fr/600955/ http://agritrop.cirad.fr/600955/1/vir001516.pdf |
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dig-cirad-fr-6009552024-01-29T04:09:21Z http://agritrop.cirad.fr/600955/ http://agritrop.cirad.fr/600955/ Alfalfa leaf curl virus is efficiently acquired by its aphid vector Aphis craccivora but inefficiently transmitted. Ryckebusch Faustine, Peterschmitt Michel, Granier Martine, Sauvion Nicolas. 2021. Journal of General Virology, 102 (2):1516, 18 p.https://doi.org/10.1099/jgv.0.001516 <https://doi.org/10.1099/jgv.0.001516> Alfalfa leaf curl virus is efficiently acquired by its aphid vector Aphis craccivora but inefficiently transmitted Ryckebusch, Faustine Peterschmitt, Michel Granier, Martine Sauvion, Nicolas eng 2021 Journal of General Virology H20 - Maladies des plantes enroulement des feuilles Aphis craccivora vecteur de maladie http://aims.fao.org/aos/agrovoc/c_4237 http://aims.fao.org/aos/agrovoc/c_30760 http://aims.fao.org/aos/agrovoc/c_8164 Alfalfa leaf curl virus (ALCV) is the first geminivirus for which aphid transmission was reported. Transmission by Aphis craccivora was determined previously to be highly specific and circulative. Using various complementary techniques, the transmission journey of ALCV was monitored from its uptake from infected plant tissues up to the head of its vector. ALCV was shown to be restricted to phloem tissues using fluorescence in situ hybridization (FISH) and electropenetrography (EPG) monitoring of virus acquisition. Furthermore, the virus is heterogeneously distributed in phloem tissues, as revealed by FISH and quantitative PCR of viral DNA acquired by EPG-monitored aphids. Despite the efficient ingestion of viral DNA, about 106 viral DNA copies per insect in a 15 h feeding period on ALCV-infected plants, the individual maximum transmission rate was 12 %. Transmission success was related to a critical viral accumulation, around 1.6×107 viral DNA copies per insect, a threshold that generally needed more than 48 h to be reached. Moreover, whereas the amount of acquired virus did not decrease over time in the whole aphid body, it declined in the haemolymph and heads. ALCV was not detected in progenies of viruliferous aphids and did not affect aphid fitness. Compared to geminiviruses transmitted by whiteflies or leafhoppers, or to luteoviruses transmitted by aphids, the transmission efficiency of ALCV by A. craccivora is low. This result is discussed in relation to the aphid vector of this geminivirus and the agroecological features of alfalfa, a hardy perennial host plant. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/600955/1/vir001516.pdf text cc_by_nc_nd info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/4.0/ https://doi.org/10.1099/jgv.0.001516 10.1099/jgv.0.001516 info:eu-repo/semantics/altIdentifier/doi/10.1099/jgv.0.001516 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1099/jgv.0.001516 |
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H20 - Maladies des plantes enroulement des feuilles Aphis craccivora vecteur de maladie http://aims.fao.org/aos/agrovoc/c_4237 http://aims.fao.org/aos/agrovoc/c_30760 http://aims.fao.org/aos/agrovoc/c_8164 H20 - Maladies des plantes enroulement des feuilles Aphis craccivora vecteur de maladie http://aims.fao.org/aos/agrovoc/c_4237 http://aims.fao.org/aos/agrovoc/c_30760 http://aims.fao.org/aos/agrovoc/c_8164 |
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H20 - Maladies des plantes enroulement des feuilles Aphis craccivora vecteur de maladie http://aims.fao.org/aos/agrovoc/c_4237 http://aims.fao.org/aos/agrovoc/c_30760 http://aims.fao.org/aos/agrovoc/c_8164 H20 - Maladies des plantes enroulement des feuilles Aphis craccivora vecteur de maladie http://aims.fao.org/aos/agrovoc/c_4237 http://aims.fao.org/aos/agrovoc/c_30760 http://aims.fao.org/aos/agrovoc/c_8164 Ryckebusch, Faustine Peterschmitt, Michel Granier, Martine Sauvion, Nicolas Alfalfa leaf curl virus is efficiently acquired by its aphid vector Aphis craccivora but inefficiently transmitted |
description |
Alfalfa leaf curl virus (ALCV) is the first geminivirus for which aphid transmission was reported. Transmission by Aphis craccivora was determined previously to be highly specific and circulative. Using various complementary techniques, the transmission journey of ALCV was monitored from its uptake from infected plant tissues up to the head of its vector. ALCV was shown to be restricted to phloem tissues using fluorescence in situ hybridization (FISH) and electropenetrography (EPG) monitoring of virus acquisition. Furthermore, the virus is heterogeneously distributed in phloem tissues, as revealed by FISH and quantitative PCR of viral DNA acquired by EPG-monitored aphids. Despite the efficient ingestion of viral DNA, about 106 viral DNA copies per insect in a 15 h feeding period on ALCV-infected plants, the individual maximum transmission rate was 12 %. Transmission success was related to a critical viral accumulation, around 1.6×107 viral DNA copies per insect, a threshold that generally needed more than 48 h to be reached. Moreover, whereas the amount of acquired virus did not decrease over time in the whole aphid body, it declined in the haemolymph and heads. ALCV was not detected in progenies of viruliferous aphids and did not affect aphid fitness. Compared to geminiviruses transmitted by whiteflies or leafhoppers, or to luteoviruses transmitted by aphids, the transmission efficiency of ALCV by A. craccivora is low. This result is discussed in relation to the aphid vector of this geminivirus and the agroecological features of alfalfa, a hardy perennial host plant. |
format |
article |
topic_facet |
H20 - Maladies des plantes enroulement des feuilles Aphis craccivora vecteur de maladie http://aims.fao.org/aos/agrovoc/c_4237 http://aims.fao.org/aos/agrovoc/c_30760 http://aims.fao.org/aos/agrovoc/c_8164 |
author |
Ryckebusch, Faustine Peterschmitt, Michel Granier, Martine Sauvion, Nicolas |
author_facet |
Ryckebusch, Faustine Peterschmitt, Michel Granier, Martine Sauvion, Nicolas |
author_sort |
Ryckebusch, Faustine |
title |
Alfalfa leaf curl virus is efficiently acquired by its aphid vector Aphis craccivora but inefficiently transmitted |
title_short |
Alfalfa leaf curl virus is efficiently acquired by its aphid vector Aphis craccivora but inefficiently transmitted |
title_full |
Alfalfa leaf curl virus is efficiently acquired by its aphid vector Aphis craccivora but inefficiently transmitted |
title_fullStr |
Alfalfa leaf curl virus is efficiently acquired by its aphid vector Aphis craccivora but inefficiently transmitted |
title_full_unstemmed |
Alfalfa leaf curl virus is efficiently acquired by its aphid vector Aphis craccivora but inefficiently transmitted |
title_sort |
alfalfa leaf curl virus is efficiently acquired by its aphid vector aphis craccivora but inefficiently transmitted |
url |
http://agritrop.cirad.fr/600955/ http://agritrop.cirad.fr/600955/1/vir001516.pdf |
work_keys_str_mv |
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_version_ |
1792500349764894720 |